In vivo regeneration of renal vessels post whole decellularized kidneys transplantation

Abstract

Nearly 50 million patients in China live with end-stage renal disease (ESRD), and only about 4000 patients may receive kidney transplantation. The purpose of this study was to investigate regeneration of renal vessels post whole decellularized kidneys transplantation in vivo. We decellularized kidneys of donor rats by perfusing a detergent through the abdominal aorta, yielding feasible extracellular matrix, confirmed for acellularity before transplantation. Based on the concept of using the body as a bioreactor, we orthotopically transplanted the kidney and ureter scaffolds in recipient rats, and found the regeneration of vessels including artery and vein in the renal sinus following a spontaneous recanalization. Although the findings only represent an initial step toward the ultimate goal of the generation of fully functional kidneys in vivo, these findings suggest that the body itself, as the bioreactor, is a viable strategy for kidney regeneration.

The effect of riboflavin/UVA cross‐linking on anti‐degeneration and promoting angiogenic capability of decellularized liver matrix

“…The other feasible one is orthotopically or heterotopically transplanting the complexes of cells and scaffolds, and then vessels would grow into the scaffold and supply nutrients and oxygen to cells. Unfortunately, previous research of our team 6 and other groups [16][17][18] suggest that few vessels or parenchymal cells but only inflammatory cells were found after a period of observation. And the speed of scaffold degradation was fast and unstable.…”

EPCs enhance angiogenesis in renal regeneration

“…Data from radionuclide renogram demonstrated enhanced restoration of renal functions. Based on these observations and relying on previous insights [16, 17], we speculated that the intensity of angiogenesis may promote renal regeneration. Impaired organs may be repaired by seeded endothelial cells to help repairing vessels, hence, organs [18].…”

Comparative study of two perfusion routes with different flow in decellularization to harvest an optimal pulmonary scaffold for recellularization

“…Preserving the biomechanical integrity, especially the integrity of blood vessels, is critical if a decellularized matrix is to serve as an effective recellularization scaffold. Most of previous studies that comparing the different perfusion protocols for decellularization of animal and human lungs, they usually only utilized small segments of lungs for their experiments, but not used the whole organs.…”

“…There was no evidence that the increased flow or pressure had deleterious effects on the vascular structures during the process of heart decellularization. 16 Preserving the biomechanical integrity, especially the integrity of blood vessels, 17 is critical if a decellularized matrix is to serve as an effective recellularization scaffold.…”