The effect of riboflavin/UVA cross‐linking on anti‐degeneration and promoting angiogenic capability of decellularized liver matrix

Abstract

Weak mechanical property and unstable degradation rate limited the application of decellularized liver matrix in tissue engineering. The aim of this study was to explore a new method for improving the mechanical properties, anti-degeneration and angiogenic capability of decellularized liver matrix. This was achieved by a novel approach using riboflavin/ultraviolet A treatment to induce collagen cross-linking of decellularized matrix. Histological staining and scanning electron microscope showed that the diameter of cross-linked fibers significantly increased compared with the control group. The average peak load and Young's modulus of decellularized matrix were obviously improved after cross-linking. Then we implanted the modified matrix into the rat hepatic injury model to test the anti-degeneration and angiogenic capability of riboflavin/UVA cross-linked decellularized liver scaffolds in vivo. The results indicated that cross-linked scaffolds degrade more slowly than those in the control group. In the experiment group, average microvessel density in the implanted matrix was higher than that in the control group since the first week after implantation. In conclusion, we initiated the method to improve the biomechanical properties of decellularized liver scaffolds by riboflavin/UVA cross-linking, and more importantly, its improvement on anti-degeneration and angiogenesis was identified. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2662-2669, 2017.

Inactivation of human plasma alters the structure and biomechanical properties of engineered tissues

“…Considering that riboflavin/UVA treatment can act as a cross-linking system able to improve the biomechanical Frontiers in Bioengineering and Biotechnology frontiersin.org rigidity of decellularized scaffolds (Xiang et al, 2017) and corneas (Aslanides et al, 2016), it might also have a positive effect on fibrin-agarose scaffolds. However, the correlation analysis revealed the opposite behavior, showing that riboflavin/UVA exposure on plasma did not preserve coagulation factor levels.…”

The inhibitory effect of carboxyl-terminated polyamidoamine dendrimers on dentine host-derived matrix metalloproteinases in vitro in an etch-and-rinse adhesive system

“…It has been reported that cross-linking by agents such as glutaraldehyde and proanthocyanidins is an effective method to improve the mechanical property of dentine collagen [6,60,61] due to the generation of additional collagen cross-links by covalent and hydrogen bonds [9][10][11]. Hence, the present research intended to determine whether or not the molecular structure of dentine collagen changed after the G4-PAMAM-COOH pre-treatment and ascertain the mechanism of interaction between G4-PAMAM-COOH and collagen, which contributed to the evaluation of the effect of G4-PAMAM-COOH on the mechanical capacity of dentine collagen.…”

Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

“…For many solid organs and tissues, canulating and perfusing the primary artery is one of the best approaches. The kidney is perfused through the renal artery [ 148 , 164 , 243 ], the liver through the portal vein and/or the hepatic artery [ 169 , 245 ], the pancreas through the splenic vein [ 99 ], the uterus through the uterine artery [ 153 ], and the corpus cavernosum through the cavernosal artery [ 157 ]. Other organs that are typical decellularized through chemical baths can be decellularized if there is a need to keep the structure intact, such as perfusing the superior mesenteric artery to decellularize the jejunum [ 144 ], the common carotid artery for the cervical esophagus [ 246 ], and the ureter for bladders [ 185 ].…”