<i>In Vivo</i> Photopharmacology Enabled by Multifunctional Fibers

Frank, James A.; Antonini, Marc Joseph; Chiang, Pai Kai; Canales, Andrés; Konrad, David; Garwood, Indie C.; Rajic, Gabriela; Koehler, Florian; Fink, Yoel; Anikeeva, Polina

doi:10.1021/acschemneuro.0c00577

Cited by 27 publications

(25 citation statements)

References 63 publications

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“…Optical fibers possess a set of attributes which include flexibility, chemical and bio-inertness, easy light coupling, and a high aspect ratio. Moreover, their widespread use across numerous fields, from telecommunications to sensing 22 , 23 and imaging in medicine 24 – 27 have brought about high tolerance, and standardized production in kilometer lengths which can be assembled into low-cost optofluidic devices with precise channel dimensions using equipment developed for telecommunications production. In previous work, we demonstrated several all-fiber optofluidic devices for biomedical applications fabricated from an array of optical fibers and capillaries.…”

Section: Introductionmentioning

confidence: 99%

A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection

Parker

Sengupta

Harish

et al. 2022

Sci Rep

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Microfluidics has emerged rapidly over the past 20 years and has been investigated for a variety of applications from life sciences to environmental monitoring. Although continuous-flow microfluidics is ubiquitous, segmented-flow or droplet microfluidics offers several attractive features. Droplets can be independently manipulated and analyzed with very high throughput. Typically, microfluidics is carried out within planar networks of microchannels, namely, microfluidic chips. We propose that fibers offer an interesting alternative format with key advantages for enhanced optical coupling. Herein, we demonstrate the generation of monodisperse droplets within a uniaxial optofluidic Lab-in-a-Fiber scheme. We combine droplet microfluidics with laser-induced fluorescence (LIF) detection achieved through the development of an optical side-coupling fiber, which we term a periscope fiber. This arrangement provides stable and compact alignment. Laser-induced fluorescence offers high sensitivity and low detection limits with a rapid response time making it an attractive detection method for in situ real-time measurements. We use the well-established fluorophore, fluorescein, to characterize the Lab-in-a-Fiber device and determine the generation of $$\sim$$ ∼ 0.9 nL droplets. We present characterization data of a range of fluorescein concentrations, establishing a limit of detection (LOD) of 10 nM fluorescein. Finally, we show that the device operates within a realistic and relevant fluorescence regime by detecting reverse-transcription loop-mediated isothermal amplification (RT-LAMP) products in the context of COVID-19 diagnostics. The device represents a step towards the development of a point-of-care droplet digital RT-LAMP platform.

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Section: Introductionmentioning

confidence: 99%

A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection

Parker

Sengupta

Harish

et al. 2022

Sci Rep

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“…Unlike other high T m metals/alloys, [ 2,18 ] a low T m of ≈156 °C enables indium to be co‐drawn with an optically transparent PC/COC waveguide (PC refractive index n PC = 1.59, T g = 150 °C; COC n COC = 1.53, T g = 158 °C). [ 2,4,19 ] The macroscale first‐step fibers are then arranged inside a COC/PC polymer cladding to form the second‐step preform which is then drawn into a microscale fiber. As these first‐step fibers can be arranged into any desired configuration, this “mix‐and‐match” approach enables straightforward customization of fibers for a given application.…”

Section: Resultsmentioning

confidence: 99%

Customizing MRI‐Compatible Multifunctional Neural Interfaces through Fiber Drawing

Antonini

Sahasrabudhe

Tabet

et al. 2021

Adv Funct Materials

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Fiber drawing enables scalable fabrication of multifunctional flexible fibers that integrate electrical, optical, and microfluidic modalities to record and modulate neural activity. Constraints on thermomechanical properties of materials, however, have prevented integrated drawing of metal electrodes with low‐loss polymer waveguides for concurrent electrical recording and optical neuromodulation. Here, two fabrication approaches are introduced: 1) an iterative thermal drawing with a soft, low melting temperature (Tm) metal indium, and 2) a metal convergence drawing with traditionally non‐drawable high Tm metal tungsten. Both approaches deliver multifunctional flexible neural interfaces with low‐impedance metallic electrodes and low‐loss waveguides, capable of recording optically‐evoked and spontaneous neural activity in mice over several weeks. These fibers are coupled with a light‐weight mechanical microdrive (1 g) that enables depth‐specific interrogation of neural circuits in mice following chronic implantation. Finally, the compatibility of these fibers with magnetic resonance imaging is demonstrated and they are applied to visualize the delivery of chemical payloads through the integrated channels in real time. Together, these advances expand the domains of application of the fiber‐based neural probes in neuroscience and neuroengineering.

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“…Researchers have been attempting to understand how our brains learn [ 114 ], memorize, and make decisions [ 115 ] when multiple signals, including electrophysical, chemical, and other signals, are comodulated. With the advancement of neuroscience, optogenetics [ 116 ], and photopharmacology [ 117 ], there is a growing need for multifunctional and biocompatible devices capable of recording multiple signals with high resolution and minimal invasion. Using biocompatible multifunctional fiber probes, researchers have recorded spinal cord [ 118 ] and brain circuits [ 53 , 54 , 119 ].…”

Section: The Materials Of Polymer Optical Fibermentioning

confidence: 99%

Biocompatible and Biodegradable Polymer Optical Fiber for Biomedical Application: A Review

et al. 2021

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This article discusses recent advances in biocompatible and biodegradable polymer optical fiber (POF) for medical applications. First, the POF material and its optical properties are summarized. Then, several common optical fiber fabrication methods are thoroughly discussed. Following that, clinical applications of biocompatible and biodegradable POFs are discussed, including optogenetics, biosensing, drug delivery, and neural recording. Following that, biomedical applications expanded the specific functionalization of the material or fiber design. Different research or clinical applications necessitate the use of different equipment to achieve the desired results. Finally, the difficulty of implanting flexible fiber varies with its flexibility. We present our article in a clear and logical manner that will be useful to researchers seeking a broad perspective on the proposed topic. Overall, the content provides a comprehensive overview of biocompatible and biodegradable POFs, including previous breakthroughs, as well as recent advancements. Biodegradable optical fibers have numerous applications, opening up new avenues in biomedicine.

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In Vivo Photopharmacology Enabled by Multifunctional Fibers

Cited by 27 publications

References 63 publications

A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection

A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection

Customizing MRI‐Compatible Multifunctional Neural Interfaces through Fiber Drawing

Biocompatible and Biodegradable Polymer Optical Fiber for Biomedical Application: A Review

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