<i>In Vivo</i>Immunomodulation and Lipid Peroxidation Activities Contributed to Chemoprevention Effects of Fermented Mung Bean against Breast Cancer

Yeap, Swee Keong; Yusof, Hamidah Mohd; Mohamad, Nurul Elyani; Beh, Boon Kee; Ho, Wan Yong; Ali, Norlaily Mohd; Alitheen, Noorjahan Banu; Koh, Soo Peng; Long, Kamariah

doi:10.1155/2013/708464

Cited by 20 publications

(19 citation statements)

References 20 publications

(30 reference statements)

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“…Upon the experimental end-point, histopathology of 4T1 MIND tumors was examined to determine if their features differed from what has been reported for 4T1 MFP tumors. Trichrome staining revealed adjacent muscle invasion ( Figure 2A) and regions of necrosis with extensive immune cell infiltration ( Figure 2B), features that have also been identified in 4T1 MFP tumors (31,32). In addition, 4T1 MIND tumors also showed abundant collagen deposition ( Figure 2C) and regions of fibrosis ( Figure 2C), two indicators of malignant progression that have not been reported for 4T1 MFP tumors as far as we are aware (33,34).…”

Section: Resultsmentioning

confidence: 66%

Intraductal Adaptation of the 4T1 Mouse Model of Breast Cancer Reveals Effects of the Epithelial Microenvironment on Tumor Progression and Metastasis

et al. 2019

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Background: Low success rates in oncology drug development are prompting re-evaluation of preclinical models, including orthotopic tumor engraftment. In breast cancer models, tumor cells are typically injected into mouse mammary fat pads (MFP). However, this approach bypasses the epithelial microenvironment, potentially altering tumor properties in ways that affect translational application. Materials and Methods: Tumors were generated by mammary intraductal (MIND) engraftment of 4T1 carcinoma cells. Growth, histopathology, and molecular features were quantified. Results: Despite growth similar to that of 4T1 MFP tumors, 4T1 MIND tumors exhibit distinct histopathology and increased metastasis. Furthermore, >6,000 transcripts were found to be uniquely up-regulated in 4T1 MIND tumor cells, including genes that drive several cancer hallmarks, in addition to two known therapeutic targets that were not up-regulated in 4T1 MFP tumor cells. Conclusion: Engraftment into the epithelial microenvironment generates tumors that more closely recapitulate the complexity of malignancy, suggesting that intraductal adaptation of orthotopic mammary models may be an important step towards improving outcomes in preclinical drug screening and development.

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Section: Resultsmentioning

confidence: 66%

Intraductal Adaptation of the 4T1 Mouse Model of Breast Cancer Reveals Effects of the Epithelial Microenvironment on Tumor Progression and Metastasis

et al. 2019

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“…Immunophenotyping of spleen CD3, CD4, and CD8 were done based on Yeap et al 21 Briefly, the harvested spleens from normal, untreated, and kefir water–treated groups were meshed, and red blood cells were lysed using lysis buffer (8 g NH 4 Cl, 1 g Na 2 EDTA, 0.1 g KH 2 PO 4 at pH 7.4). The cells were washed with PBS and stained with the CD3, CD4, and CD8 antibodies (Abcam, USA; Catalog No: ab11089, ab25475, ab22378) for 2 hours.…”

Section: Methodsmentioning

confidence: 99%

The Antimetastatic and Antiangiogenesis Effects of Kefir Water on Murine Breast Cancer Cells

et al. 2016

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Background. Kefir is a unique cultured product that contains beneficial probiotics. Kefir culture from other parts of the world exhibits numerous beneficial qualities such as anti-inflammatory, immunomodulation, and anticancer effects. Nevertheless, kefir cultures from different parts of the world exert different effects because of variation in culture conditions and media. Breast cancer is the leading cancer in women, and metastasis is the major cause of death associated with breast cancer. The antimetastatic and antiangiogenic effects of kefir water made from kefir grains cultured in Malaysia were studied in 4T1 breast cancer cells. Methods. 4T1 cancer cells were treated with kefir water in vitro to assess its antimigration and anti-invasion effects. BALB/c mice were injected with 4T1 cancer cells and treated orally with kefir water for 28 days. Results. Kefir water was cytotoxic toward 4T1 cells at IC50 (half-maximal inhibitory concentration) of 12.5 and 8.33 mg/mL for 48 and 72 hours, respectively. A significant reduction in tumor size and weight (0.9132 ± 0.219 g) and a substantial increase in helper T cells (5-fold) and cytotoxic T cells (7-fold) were observed in the kefir water–treated group. Proinflammatory and proangiogenic markers were significantly reduced in the kefir water–treated group. Conclusions. Kefir water inhibited tumor proliferation in vitro and in vivo mainly through cancer cell apoptosis, immunomodulation by stimulating T helper cells and cytotoxic T cells, and anti-inflammatory, antimetastatic, and antiangiogenesis effects. This study brought out the potential of the probiotic beverage kefir water in cancer treatment.

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“…Also, a non-protein amino acid, gamma-aminobutyric acid (GABA) has been shown to demonstrate anti-inflammatory activity. In vivo experiments using GABA-rich extracts were conducted on female Balb/c mice inoculated with Yac-1 and 4T1 carcinogenic cell lines [ 34 ]. Mice supplemented with diets of either 200 mg/kg or 1000 mg/kg body weight of GABA exhibited delayed tumour formation.…”

Section: Cancer Development and Mechanisms Of Inhibitionmentioning

confidence: 99%

“…This was correlated with high anti-cancer cytokine levels, splenic T cell populations, splenocyte cytotoxicity and spleen/tumour antioxidant levels. Mitotic divisions in spleen were also reduced which was accompanied by a reduction in antioxidant and nitric oxide levels [ 34 ].…”

Section: Cancer Development and Mechanisms Of Inhibitionmentioning

confidence: 99%

Inhibitory Effects of Pulse Bioactive Compounds on Cancer Development Pathways

et al. 2018

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Previous studies suggest that pulses may have the potential to protect against cancer development by inhibiting pathways that result in the development of cancer. These pathways include those that result in inflammation, DNA damage, cell proliferation, and metastasis. Other studies have demonstrated extracts from pulses have the capacity to induce apoptosis specifically in cancer cells. Compounds reported to be responsible for these activities have included phenolic compounds, proteins and short chain fatty acids. The majority of the studies have been undertaken using in vitro cell culture models, however, there are a small number of in vivo studies that support the hypothesis that pulse consumption may inhibit cancer development. This review highlights the potential benefit of a diet rich in pulse bioactive compounds by exploring the anti-cancer properties of its polyphenols, proteins and short chain fatty acids.

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In VivoImmunomodulation and Lipid Peroxidation Activities Contributed to Chemoprevention Effects of Fermented Mung Bean against Breast Cancer

Cited by 20 publications

References 20 publications

Intraductal Adaptation of the 4T1 Mouse Model of Breast Cancer Reveals Effects of the Epithelial Microenvironment on Tumor Progression and Metastasis

Intraductal Adaptation of the 4T1 Mouse Model of Breast Cancer Reveals Effects of the Epithelial Microenvironment on Tumor Progression and Metastasis

The Antimetastatic and Antiangiogenesis Effects of Kefir Water on Murine Breast Cancer Cells

Inhibitory Effects of Pulse Bioactive Compounds on Cancer Development Pathways

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