In vivo evaluation of hybrid patches composed of PLA based copolymers and collagen/chondroitin sulfate for ligament tissue regeneration

Abstract

Biomaterials for soft tissues regeneration should exhibit sufficient mechanical strength, demonstrating a mechanical behavior similar to natural tissues and should also promote tissues ingrowth. This study was aimed at developing new hybrid patches for ligament tissue regeneration by synergistic incorporation of a knitted structure of degradable polymer fibers to provide mechanical strength and of a biomimetic matrix to help injured tissues regeneration. PLA- Pluronic (PLA-P) and PLA-Tetronic (PLA-T) new copolymers were shaped as knitted patches and were associated with collagen I (Coll) and collagen I/chondroitine-sulfate (Coll CS) 3-dimensional matrices. In vitro study using ligamentocytes showed the beneficial effects of CS on ligamentocytes proliferation. Hybrid patches were then subcutaneously implanted in rats for 4 and 12 weeks. Despite degradation, patches retained strength to answer the mechanical physiological needs. Tissue integration capacity was assessed with histological studies. We showed that copolymers, associated with collagen and chondroitin sulfate sponge, exhibited very good tissue integration and allowed neotissue synthesis after 12 weeks in vivo. To conclude, PLA-P/CollCS and PLA-T/CollCS hybrid patches in terms of structure and composition give good hopes for tendon and ligament regeneration. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1778-1788, 2017.

A New Bone Substitute Developed from 3D-Prints of Polylactide (PLA) Loaded with Collagen I: An In Vitro Study

“…Until now, no such approach has been tested. Pinese et al combined PLA polymers as knitted patches with collagen/chondroitin sulfate for ligament regeneration [ 52 ]. Heo et al combined PLA with gelatin hydrogels and showed that human adipose-derived stem cells can be drifted to osteogenic differentiation and suggest this material for bone tissue engineering [ 53 ].…”

Fabrication, Crystalline Behavior, Mechanical Property and In-Vivo Degradation of Poly(l–lactide) (PLLA)–Magnesium Oxide Whiskers (MgO) Nano Composites Prepared by In-Situ Polymerization

“…Then, a remarkable discrepancy with a sharp decline of M η was observed for the third and sixth month, suggesting the occurrence of rapid decomposition for PLLA1. The reversal of the degrading rate in PLLA1 probably resulted from the increment of the pH value due to the dissolution of more hydrophilic MgO whiskers in relatively long-term degradation, which accelerated the hydrolysis of PLLA since it had a higher decomposing rate in an alkaline environment [37].…”

Investigation on the Durability of PLA Bionanocomposite Fibers Under Hygrothermal Conditions

“…In Figures 2A,B, there is an increasing evolution of SI curves with time for all PLA fibers whatever the temperature meaning that the degradation mechanism predominantly occurring in the matrix is chain scission (Gajjar and King, 2014;Gil-Castell et al, 2014). Indeed, the literature (Elsawy et al, 2017) reported that under humid conditions, hydrolysis reactions occur between PLA ester groups and water molecules resulting in chain scission forming chain segments with low molecular weight (Girdthep et al, 2016;Lins et al, 2016;Lorenzo et al, 2016;Mohammad et al, 2016;Pinese et al, 2016;Stloukal et al, 2016;Yang et al, 2016). Moreover, the hydrolysis of PLA bionanocomposites is strongly dependent on the intrinsic characteristics of PLA matrix, the nature of fillers, their dispersion in the polymer and the environment conditions (humidity and temperature) (Zhou and Xanthos, 2008;Maharana et al, 2009).…”