<i>In vivo</i> biocompatibility and time‐dependent changes in mechanical properties of woven collagen meshes: A comparison to xenograft and synthetic mid‐urethral sling materials

Chapin, Katherine; Khalifa, Ahmad; Mbimba, Thomas; McClellan, Phillip; Anderson, James M.; Novitsky, Yuri W.; Hijaz, Adonis; Akkuş, Ozan

doi:10.1002/jbm.b.34138

Cited by 14 publications

(20 citation statements)

References 21 publications

(30 reference statements)

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“…In summary, the LFE-FRESH process is versatile, low-cost, and compatible with open-source 3D printing software and hardware. The chemical cross-linking treatment improves fiber-gel bonding using EDC+NHS, which was adopted because EDC+NHS cross-linking has previously been used in ELAC production. , Additionally, it does not alter the color and transparency of hydrogels, which was beneficial in assessing print fidelity in this study. In addition, other types of chemical cross-linker, such as transglutaminase, which could be used in the presence of cells, or genipin, could be tested with the LFE-FRESH technique in the future.…”

Section: Resultsmentioning

confidence: 99%

Long-Fiber Embedded Hydrogel 3D Printing for Structural Reinforcement

Sun

Tashman

Shiwarski

et al. 2021

ACS Biomater. Sci. Eng.

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Hydrogels are candidate building blocks in a wide range of biomaterial applications including soft and biohybrid robotics, microfluidics, and tissue engineering. Recent advances in embedded 3D printing have broadened the design space accessible with hydrogel additive manufacturing. Specifically, the Freeform Reversible Embedding of Suspended Hydrogels (FRESH) technique has enabled the fabrication of complex 3D structures using extremely soft hydrogels, e.g., alginate and collagen, by assembling hydrogels within a fugitive support bath. However, the low structural rigidity of FRESH printed hydrogels limits their applications, especially those that require operation in nonaqueous environments. In this study, we demonstrated long-fiber embedded hydrogel 3D printing using a multihead printing platform consisting of a custom-built fiber extruder and an open-source FRESH bioprinter with high embedding fidelity. Using this process, fibers were embedded in 3D printed hydrogel components to achieve significant structural reinforcement (e.g., tensile modulus improved from 56.78 ± 8.76 to 382.55 ± 25.29 kPa and tensile strength improved from 9.44 ± 2.28 to 45.05 ± 5.53 kPa). In addition, we demonstrated the versatility of this technique by using fibers of a wide range of sizes and material types and implementing different 2D and 3D embedding patterns, such as embedding a conical helix using electrochemically aligned collagen fiber via nonplanar printing. Moreover, the technique was implemented using low-cost material and is compatible with open-source software and hardware, which facilitates its adoption and modification for new research applications.

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Section: Resultsmentioning

confidence: 99%

Long-Fiber Embedded Hydrogel 3D Printing for Structural Reinforcement

Sun

Tashman

Shiwarski

et al. 2021

ACS Biomater. Sci. Eng.

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“…Development of variable aged in vivo models for SUI may help understanding the pathophysilogy of birth trauma and the longterm regeneration and repair . Reliable and durable low cost animal model may help in testing promising novel regenerative treatments for SUI . The success to create a reliable VD mouse model may help explore the individualized genetic variabilities and possible novel microenviromental manuvers in the treatment of SUI patients …”

Section: Discussionmentioning

confidence: 99%

“…17 Reliable and durable low cost animal model may help in testing promising novel regenerative treatments for SUI. 20,21 The success to create a reliable VD mouse model may help explore the individualized genetic variabilities and possible novel microenviromental manuvers in the treatment of SUI patients. 22 This is the highest powered rodent study using the VD model of simulated birth trauma to date.…”

Section: Discussionmentioning

confidence: 99%

The vaginal distention model in mice is not a reliable model of simulated birth trauma‐induced stress urinary incontinence

Hassan

Galante

Kavran

et al. 2018

Neurourology and Urodynamics

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Aims Vaginal distention (VD) is a validated model of birth‐related trauma in rats. Recently a mouse VD model was reported. Our study was originally conducted to evaluate the impact of age on VD in mice. This manuscript describes the study and reports on the lack of reproducibility of VD models in mice. Methods We utilized female C57BL/6 mice. A total of 190, 12‐weeks old mice, were randomized into VD and sham groups. We inflated a modified Foley's balloon with 0.3 mL for 1 h inside the mice vagina. Afterwards, we measured the leak point pressure (LPP) at defined timepoints (0, 4, 10, 20, or 40 days). We randomized another 190, 40‐week old, C57BL/6 mice into either VD or sham groups. We used an extra 20 mice as age − matched controls. Results In both 12 and 40 weeks‐old mice, LPP was significantly decreased versus the negative controls at day 0. Additionally, in both 12 and 40 weeks‐old mice, the decrease in LPP was significantly higher in the VD group compared to the sham group at day 0. However, the LPP results were comparable between VD and sham at any other time point thereafter. Furthermore, there was no significant change in LPP values between instrumented (VD and sham) mice and control mice at any time after day 0. Conclusions The VD models previously described is not a reproducible model for the study of VD with large number of mice. Our results, unfortunately, do not support its use to study VD injury in mice.

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“…Another long‐lasting absorbable material (highly aligned collagen threads crosslinked using either genipin or 1‐ethyl‐3‐(3‐dimethylaminopropyl) carboiimide and N‐hydroxy succinimide (EDC/NHS)) has been evaluated in a rat model, showing good mechanical properties up to 5 months and good biocompatibility 28 …”

Section: Intrinsic Materials Considerations Regarding Polypropylene A...mentioning

confidence: 99%

Is polypropylene mesh material fundamentally safe for use as a reconstructive material in vaginal surgery: ICI‐RS 2019?

Tayrac¹,

Kirschner‐Hermanns²,

Veit‐Rubin

et al. 2020

Neurourology and Urodynamics

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Polypropylene (PP) mesh has been used as a primary or adjuvant material for vaginal reconstruction for both stress urinary incontinence (SUI) and pelvic organ prolapse (POP) for decades. Whether polypropylene is the optimal material for such indications has been called into question by clinicians, regulatory agencies and the public in several countries around the world. This paper is a report of presentations and subsequent discussion at the annual International Consultation on Incontinence Research Society (ICI-RS) Meeting in June 2019 in Bristol, UK on the proposal "Is polypropylene mesh material fundamentally safe for use as a reconstructive material in vaginal surgery?" in which several of the salient issues were presented and discussed.

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In vivo biocompatibility and time‐dependent changes in mechanical properties of woven collagen meshes: A comparison to xenograft and synthetic mid‐urethral sling materials

Cited by 14 publications

References 21 publications

Long-Fiber Embedded Hydrogel 3D Printing for Structural Reinforcement

Long-Fiber Embedded Hydrogel 3D Printing for Structural Reinforcement

The vaginal distention model in mice is not a reliable model of simulated birth trauma‐induced stress urinary incontinence

Is polypropylene mesh material fundamentally safe for use as a reconstructive material in vaginal surgery: ICI‐RS 2019?

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