In vivo anti‐inflammatory activity of Fabaceae species extracts screened by a new ex vivo assay using human whole blood

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Hops (Humulus lupulus L.) are edible flowers commonly used to add flavour and aroma to beer, besides they have rich chemical diversity and medicinal potential. In this work, an ex vivo anti‐inflammatory assay via the LPS‐induced signalling pathway and metabolomics approaches were performed to evaluate the ability of hops to inhibit the production of prostaglandin E2 (PGE2) inflammatory mediator and analyze which metabolites produced by the nine different hop cultivars are potential anti‐inflammatory markers. Columbus, Chinook and Hallertau Mittelfrüh hop cultivars yielded extracts with PGE2 release inhibition rates of 86.7, 92.5 and 73.5 %, respectively. According to the multivariate statistical analysis, the majority of the metabolites correlated with the activity were prenylated phloroglucinol and phenolic homologs. These results suggest promissory anti‐inflammatory hop metabolites.

Section: Resultsmentioning

confidence: 89%

“…The anti‐inflammatory assay was conducted as follows [23] . Briefly, the heparinized human blood (200 μL) was used for the quantitative evaluation of chemical mediators in plasma.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Experimental Design and Ex Vivo Anti-inflammatory Assaymentioning

confidence: 99%

Section: Quantification Of Pge2 By Uplc/ms/ms Analysis and Statistica...mentioning

confidence: 99%

See 3 more Smart Citations

Anti‐Inflammatory Markers of Hops Cultivars (Humulus lupulus L.) Evaluated by Untargeted Metabolomics Strategy

Nicácio

Ferreira

Katchborian-Neto

et al. 2022

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Anti‐Inflammatory Activity of Lauraceae Plant Species and Prediction Models Based on Their Metabolomics Profiling Data

Alcântara

Katchborian-Neto

Garcia

et al. 2023

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The Lauraceae is a botanical family known for its anti‐inflammatory potential. However, several species have not yet been studied. Thus, this work aimed to screen the anti‐inflammatory activity of this plant family and to build statistical prediction models. The methodology was based on the statistical analysis of high‐resolution liquid chromatography coupled with mass spectrometry data and the ex vivo anti‐inflammatory activity of plant extracts. The ex vivo results demonstrated significant anti‐inflammatory activity for several of these plants for the first time. The sample data were applied to build anti‐inflammatory activity prediction models, including the partial least square acquired, artificial neural network, and stochastic gradient descent, which showed adequate fitting and predictive performance. Key anti‐inflammatory markers, such as aporphine and benzylisoquinoline alkaloids were annotated with confidence level 2. Additionally, the validated prediction models proved to be useful for predicting active extracts using metabolomics data and studying their most bioactive metabolites.

The First Comprehensive Chemical Profiling of Vachellia gummifera (Willd.) Kyal. & Boatwr., a Plant with Medicinal Value

Kisiriko,

Noleto‐Dias,

Bitchagno

et al. 2024

Vachellia gummifera (Willd.) Kyal. & Boatwr. is a medicinal plant endemic to Morocco that has no documented studies on its chemical composition. In this study, the chemical composition of the water/methanol (4:1) extracts of air‐dried leaf and stem samples of Moroccan V. gummifera was determined using UHPLC‐MS and NMR. In total, over 100 metabolites were identified in our study. Pinitol was the major compound in both the leaf and stem extracts, being significantly more abundant in the former. Asparagine and 3‐hydroxyheteroendrin were the second most abundant compounds in the stem and leaf extracts, respectively, though both compounds were present in each tissue. The other compounds included flavonoids based on quercetin, and phenolic derivatives. Eucomic acid, only identified in the stems and was the major aromatic compound distinguishing the leaf and stem profiles. Quercetin 3‐O‐(6′′‐O‐malonyl)‐β‐D‐glucopyranoside was identified as the major flavonoid in the leaves but was also present in the stems. Other malonylated derivatives that were all flavonol glycosides based on myricetin, kaempferol, and isorhamnetin in addition to quercetin were also identified. This is the first report of eucomic acid and malonylated compounds in Vachellia species. This report provides valuable insights into the chemotaxonomic significance of the Vachellia genus.