In vivo anti‐ageing activity of cream containing niosomes loaded with purple glutinous rice (Oryza sativa Linn.) extract

Abstract

OBJECTIVE: To evaluate the anti-ageing activity of cream containing the methanolic purple glutinous rice extract loaded in niosomes. METHODS: The in vitro biological activities of the purple glutinous rice extracted by methanol maceration were determined. The extract loaded in niosomes and the cream containing the niosomes were developed. The in vivo anti-ageing activity in 20 human volunteers including skin hydration, pigmentation, roughness and elasticity after daily application for 28 days compared to at initial was evaluated by Corneometer, Mexameter, Visiometer and Cutometer, respectively. RESULTS: The purple glutinous rice extract showed free radical scavenging (SC 50), lipid peroxidation inhibition (IPC 50), metal ion chelating (CC 50) and tyrosinase inhibition (IC 50) values at 32.31 AE 1.28, 57.40 AE 2.12, 85.05 AE 5.43 and 43.89 AE 2.14 mg/mL which were 0.00031, 0.011, 0.0078 and 0.0016 times of the standards (0.01 AE 0.00, 0.62 AE 0.14, 0.66 AE 0.05 and 0.07 AE 0.01), respectively. The purple glutinous rice extract contained 0.35 µg of anthocyanin/1 mg of the extract determined by HPLC. After loaded in niosomes, the solubility of the extract was not only increased in various solvents, but also the chemical stability in different environments (weak base, reducing agent and acid salt) was improved. The cream formulation containing niosomes loaded with 1%w/v of the purple glutinous rice extract indicated the anthocyanin remaining percentages after 6 cycles of heating and cooling test at 52.28% of the initial. For in vivo antiageing activities, cream containing niosomes loaded with the extract gave significant decreased melanin index and skin roughness reduction of À14.05 and À9.95% of the initial, respectively. The % changes of the increased skin hydration, skin elastic extension and skin elastic recovery when applied on human volunteers' skin with this formulation were +48.73, À24.51 and +35.98%, respectively. CONCLUSION: The cream containing niosomes loaded with the 1%w/v methanolic purple glutinous rice extract gave not only the suitable in vitro antioxidant activity and physical stability of the active anthocyanin, but also the superior in vivo anti-ageing activity on human skin compared to the cream base and before application which can be further developed as a novel anti-ageing cosmeceutical product.

Ethnomedicinal Plants in Herbal Remedies Used for Treatment of Skin Diseases by Traditional Healers in Songkhla Province, Thailand

“…In addition, the antimicrobial activity of Oryza sativa L. against fungi [ 25 ] and viruses [ 26 ] has been revealed. Cream containing niosomes loaded with purple glutinous rice ( Oryza sativa L.) extract possessed anti-aging activity on human skin [ 27 ]. The antioxidative and immunomodulatory properties of Oryza sativa L. crude extract reduced the severity of psoriasis [ 28 ].…”

The Potential of High-Anthocyanin Purple Rice as a Functional Ingredient in Human Health

“…Anthocyanin extract used in cream exhibited in vitro antioxidant activity and in vivo anti-ageing activity on human skin [139] Rice bran extracts containing anthocyanins have been investigated as ingredients in the cosmetic formulations that exhibit antioxidant capacity [140] Purple rice anthocyanin is used in the colorimetric sensing of Al +3…”

Research on antioxidant performance of diglucosyl gallic acid and its application in emulsion cosmetics

“…The skin colour was tested by Colorimeter CL400 and summarised in Table 3. Larger ITA values mean lighter skin colour [19]; the black red pigment was tested by Mexameter 18, where higher test values represent higher skin black red pigment value [20].…”