In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells

Abstract

In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity.

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

“…Our findings may indicate a reduction in systemic inflammation in obese animals treated with DDW. Perhaps this result is due to the ability of deuterium to slow down or speed up the reaction rate of intracellular processes include decreased systemic oxidative stress resulting in restoration of the normal functioning of the liver, adipose and muscle tissues and/or an influence of DDW on the balance between the production of pro-and anti-inflammatory cytokines [29,30]. DDW has previously been shown to have chirality capabilities mediated, partially or completely by alterations in metabolic pathways in live systems [18].…”

“…The deuterium content in the water was measured by multipass laser absorption spectroscopy on an Isotopic Water Analyzer-912-0032 (Los Gatos Research, Inc., San Jose, CA, USA). Detailed description of the method was presented in previous studies [29,30].…”

“…Chemical analysis of water with different deuterium ratios was performed by inductively coupled plasma-mass spectrometry on an ICP-QMS Agilent 7500CE spectrometer (Agilent Technologies, Santa Clara, CA, USA). Detailed description of the methods was presented in previous studies [29,30]. Calibration solutions with a high range of element concentrations (from 0.1 µg·L −1 to 100 µg·L −1 ) were used to calibrate the device.…”

“…Limited data have shown that deuterium in aqueous solutions can stimulate or inhibit metabolic processes in living systems [23][24][25][26][27]. As mentioned above, DDW has a number of unexpected biological properties, including antitumor [28], antidotal [29] and metabolic effects [30]. However, whether deuterium is involved in the regulation of biological properties of in-vivo models has not been fully characterized [26,27].…”

Comparative Analysis of the Different Dyes’ Potential to Assess Human Normal and Cancer Cell Viability In Vitro under Different D/H Ratios in a Culture Medium

“…A standard protocol was used for Alamar Blue assay [30]. In brief, 10% of Alamar Blue (redox indicator; ermo Fisher, USA) was added to the culture medium at 24 h and 72 h and incubated for 3 h [31]. Reduced Alamar Blue was detected at 540 nm vs 630 nm using a Labsystems Multiskan PLUS spectrofluorimeter (USA).…”