In vitro sealing of iatrogenic fetal membrane defects by a collagen plug imbued with fibrinogen and plasma

Abstract

These in vitro experiments suggest that the addition of fibrinogen and plasma may enhance the sealing efficacy of collagen plugs in closing iatrogenic fetal membrane defects.

Tissuepatch is biocompatible and seals iatrogenic membrane defects in a rabbit model

“…The second sealant is an off‐the‐shelf collagen (Lyostypt) imbued with fibrinogen. This was tested before in vitro in human amniotic membranes and showed improved sealing capabilities . The third sealant is Tissuepatch, which is a thin film and clinically is used to obtain an airtight sealant in thoracoscopic surgeries.…”

“…This was tested before in vitro in human amniotic membranes and showed improved sealing capabilities. 12 The third sealant is Tissuepatch, which is a thin film and clinically is used to obtain an airtight sealant in thoracoscopic surgeries. The fourth sealant is Duraseal, which is used in neurosurgery for sealing dura mater defects and preventing cerebrospinal fluid leakage.…”

Collagen plug sealing of iatrogenic fetal membrane defects after fetoscopic surgery for congenital diaphragmatic hernia

“…Although in‐vitro data suggest proliferation of fetal membranes that were supported by collagen, it might be that this does not happen in vivo , at least using current techniques. We and others have tried to improve membrane sealing by adding fibrinogen and plasma, platelet‐rich plasma or cultured amnion cells or by substituting the collagen plug by a matrix derived from fetal membranes.…”

A collagen plug with shape memory to seal iatrogenic fetal membrane defects after fetoscopic surgery

“…Liekens et al showed by immunohistochemistry that enrichment of collagen plugs with platelets and allogeneic amniotic fluid cells increased cell proliferation in the center of collagen plugs sealing fetal membrane defects in rabbits, but did not assess sealing properties [ 24 ]. Engels et al reported that a collagen plug imbued with fibrinogen and plasma reduced amniotic fluid leakage in an ex vivo set-up approximately 35% better than a control plug [ 25 ]. Other studies used polymer based patches like Tissuepatch in vivo [ 26 ], bioadhesive-coated silicone patches [ 27 ] and fetal membrane patches ex vivo [ 28 ].…”