Tissuepatch is biocompatible and seals iatrogenic membrane defects in a rabbit model

Abstract

Tissuepatch had the best biocompatibility and efficacy in sealing an iatrogenic fetal membrane defect in the pregnant rabbit compared to other readily available sealants.

Potential sealing and repair of human FM defects after trauma with peptide amphiphiles and Cx43 antisense

“…However, the link between Cx43 expression and tissue healing has only recently been established and the results of sealing membranes with biomaterials have been variable. 8,10,11 One difficulty is that there is no ideal in vivo model which allows examination of the synthetic material in a challenging wet and highly charged AF microenvironment. Model systems for in vitro performance assessment should be designed to include attachment of the membranes to the uterine wall since membrane bonding will accomplish greater tensile strength and firm adhesion.…”

“…There have been several previous studies that have described the use of synthetic materials or medical adhesives as promising agents to seal FM defects. However, the link between Cx43 expression and tissue healing has only recently been established and the results of sealing membranes with biomaterials have been variable 8,10,11 . One difficulty is that there is no ideal in vivo model which allows examination of the synthetic material in a challenging wet and highly charged AF microenvironment.…”

“…For example, platelet or fibrin‐based strategies, gelatin or collagen plugs, cyanoacrylate or surgical glues are limited however, with around 51% to 61% fetal survival rate 3‐7 . Strategies with collagen with fibrinogen plugs in the mid‐gestational rabbit model, or from porcine small intestine and decelullarised human amnion are imperfect 8‐10 . Many of these approaches suffer from poor adhesion to wet tissues, mismatch of mechanical properties, rapid swelling or degradation due to protease activity in the AF, cytotoxicity, and severe iatrogenic complications.…”

“…[3][4][5][6][7] Strategies with collagen with fibrinogen plugs in the mid-gestational rabbit model, or from porcine small intestine and decelullarised human amnion are imperfect. [8][9][10] Many of these approaches suffer from poor adhesion to wet tissues, mismatch of mechanical properties, rapid swelling or degradation due to protease activity in the AF, cytotoxicity, and severe iatrogenic complications. Whilst mussel-mimetic tissue adhesives strongly adhere to elastomeric membranes and demonstrate good adhesion under wet conditions without swelling, degradation, cytoxicity, it is not known whether the repaired FM defect could support mechanical loads and withstand the repetitive effects of mechanical stretch or inflation applied by a bioreactor device long term.…”

A collagen plug with shape memory to seal iatrogenic fetal membrane defects after fetoscopic surgery

“…Engels et al reported that a collagen plug imbued with fibrinogen and plasma reduced amniotic fluid leakage in an ex vivo set-up approximately 35% better than a control plug [ 25 ]. Other studies used polymer based patches like Tissuepatch in vivo [ 26 ], bioadhesive-coated silicone patches [ 27 ] and fetal membrane patches ex vivo [ 28 ]. Alternative techniques included the use of sealants and tissue adhesives, such as Duraseal in rabbits [ 26 ], fibrin glue and mussel mimetic tissue adhesive both in vivo and ex vivo [ [29] , [30] , [31] ].…”