In vitroRegeneration of Oil Yielding Plants-A Review

Baghel, Sapna; Bansal, Y. K.

doi:10.1080/0972060x.2014.971068

Cited by 3 publications

(2 citation statements)

References 88 publications

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“…Arg. ), energetic and oil plants, and some vegetatively propagated vegetable crops (such as Asparagus officinalis L. and Solanum tuberosum L.) (Dickson et al 2011;Kumar and Reddy 2011;Zhang et al 2012;Baghel and Bansal 2015). For this purpose, explants are cultured on synthetic media in growth rooms or phytotrons, and in controlled environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

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Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

Miler

Kulus

Wozny

et al. 2018

In Vitro Cell.Dev.Biol.-Plant

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In the present study, the applicability of four wide-spectrum light-emitting diodes (LEDs) emitting warm light (AP67, AP673L, G2, and NS1) was determined for the micropropagation of five popular ornamental plant species: Chrysanthemum × grandiflorum, Gerbera jamesonii, Heuchera × hybrida, Ficus benjamina, and Lamprocapnos spectabilis. Plantlets were grown in a growth room with a 16-h photoperiod. The photosynthetic photon flux density was set at 62-65 μmol m −2 s −1. The composition of the media and subculture timing were adjusted to the needs of each species tested. The results were compared to the cool daylight-emitting fluorescent (FL) control (TLD 36W/54). In most of the species studied (except for F. benjamina), the highest propagation ratios, or ratios similar to the FL control, were observed under the red-and far-red-abundant G2 LEDs. NS1 spectrum (with the highest proportion of blue and green light) was also efficient for G. jamesonii and L. spectabilis, and it provided a similar propagation ratio as the FL control. Light quality affected shoot length, number of leaves, callus regeneration, and the biosynthesis of chlorophyll. This influence, however, was species-dependent. Lighting conditions did not affect the dry matter and rooting in most of the species tested, except for G. jamesonii. The substitution of FLs with G2 LEDs can result in a 50% reduction of annual electricity costs, while the application of NS1 lamps can generate savings of up to 75%. In conclusion, the G2 LED lighting system seemed to be the most suitable in terms of propagation efficiency, plantlet quality, and cost reduction.

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Section: Introductionmentioning

confidence: 99%

“…Previous studies led to the conclusion that each plant species requires a specific wavelength range for optimal growth (Gupta and Jatothu 2013;Baghel and Bansal 2015). However, companies producing plants in vitro usually propagate many different species, and the profile of their production changes as the year goes by.…”

Section: Introductionmentioning

confidence: 99%

Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

Miler

Kulus

Wozny

et al. 2018

In Vitro Cell.Dev.Biol.-Plant

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An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines

Figueroa-Varela,

Susunaga-Gómez,

Restrepo-Osorio

et al. 2023

BMC Res Notes

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Objective The purpose of this study was to develop a method for the isolation, culture, and PEG-mediated protoplast transfection from leaves of in vitro-grown plants of Ricinus communis. Results Factors such as the enzymatic composition and the incubation time were evaluated. The enzymatic solution, containing 1.6% Cellulase-R10 and 0.8% Macerozyme-R10, with 16 h of incubation, was the best condition to achieve a high protoplast yield (481.16 × 104 protoplasts/g FW) with a high percentage of viability (95%). The combination and concentration of enzymes have been shown to affect the protoplast isolation efficiency significantly. Furthermore, we found that a higher number of protoplasts (8.5 × 105 protoplast/g FW) was obtained at a longer incubation time, but their viability decreased. We obtained a simple and efficient protocol to isolate protoplast from Ricinus communis leaves and culture. A PEG-mediated protoplast transfection protocol was also established to introduce plasmid DNA into Ricinus communis genotypes cultivated in Colombia. Thus, strengthening advances in the genetic improvement processes for this crop are presented.

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Biochemical and Molecular Effects of Phoenix dactylifera and Ziziphus spina-christi Extracts on Candida albicans

Al-Ali¹,

Al-Judaibi²

2019

JBM

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Our overall knowledge of the medicinal uses of plants suggests that natural compounds could be useful in the treatment of cutaneous fungal infections in tropical regions. Furthermore, the possibilities of treatment using plant extracts may be even broader than is already known when one considers plants that have not been extensively studied in this context, such as the regional species Phoenix dactylifera and Ziziphus spina-christi. This study compared the antimicrobial activity of Phoenix dactylifera and Ziziphus spina-christi extracts in terms of their biochemical and molecular effects on Candida albicans (ATCC CA 10231). These effects included altered levels of intracellular sterols, changes in the permeability of the cell membrane, and changes in the TEF1: QRTTEF1, CaERG1: ERG1, CdERG12: CdERG1, and ERG25: ERG25 genes. Scanning electron microscopy (SEM) was used to identify morphological characteristics, and energy-dispersive X-ray spectroscopy (EDAX) analyses were conducted. In treated samples, the SEM and EDAX analyses showed cell cavities and shrinkage of the cell wall, and the number of cells was reduced to only a few abnormal cells as compared with that in the untreated samples. Yttrium was detected in the cells treated with Z. spina-christi, and high levels of osmium were detected in the cells treated with P. dactylifera. Compared with control cells, cells exposed to the concentration 150 µl/ml of Z. spina-christi extract had an average sterol concentration that was nearly 3 times higher, while the concentration was 5.5 times higher for cells treated with the 150 µl/ml of P. dactylifera extract. The ethanol extracts affected the permeability of C. albicans cell membrane. Gene sequencing showed gaps and mismatches in the ERG1, ERG12, and ERG25 genes after treatment with P. dactylifera and Z. spina-christi extracts compared with that in the controls. The results were highly significant (p ≤ 0.

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In vitroRegeneration of Oil Yielding Plants-A Review

Cited by 3 publications

References 88 publications

Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines

Biochemical and Molecular Effects of <i>Phoenix dactylifera</i> and <i>Ziziphus spina-christi</i> Extracts on <i>Candida albicans</i>

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In vitroRegeneration of Oil Yielding Plants-A Review

Cited by 3 publications

References 88 publications

Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines

Biochemical and Molecular Effects of &lt;i&gt;Phoenix dactylifera&lt;/i&gt; and &lt;i&gt;Ziziphus spina-christi&lt;/i&gt; Extracts on &lt;i&gt;Candida albicans&lt;/i&gt;

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Biochemical and Molecular Effects of <i>Phoenix dactylifera</i> and <i>Ziziphus spina-christi</i> Extracts on <i>Candida albicans</i>