<i>In vitro</i>propagation of<i>Hedychium coronarium</i>Koen. through axillary bud proliferation

Parida, R.K.; Mohanty, Sujata; Nayak, Sanghamitra

doi:10.1080/11263504.2012.748102

Cited by 18 publications

(8 citation statements)

References 34 publications

(26 reference statements)

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“…However, further study is necessary to substantiate this assumption. In this study, we could achieve 100 % outdoor plant establishment in clay pots without any acclimatization stage, which is better compared to other reports of acclimatization for H. coronarium (65 %, Bisht et al 2012), (95 %, Verma andBansal 2012) and (80 %, Parida et al 2013). …”

Section: Resultssupporting

confidence: 47%

“…Rhizome with a sprouted bud size of less than 0.5 cm failed to show any response, while explants having a sprouted bud more than 1.5 cm failed to develop multiple shoots irrespective of the media used (Table 1). The report of Parida et al (2013) is silent about two vital points, which include the % axenic culture establishment as well as the size of the explants used for culture initiation.…”

Section: Resultsmentioning

confidence: 99%

“…In the present study, the sprouted meristematic rhizome buds were chosen as explants as they are less susceptible to genetic instability during in vitro regeneration of plants and thus have advantages over earlier axenic shoot tip explants (Bisht et al 2012), indirect somatic embryogenesis (Huang and Tsai 2002;Verma and Bansal 2012) or zygotic embryos (Xiong et al 2011) mediated plant regeneration methods. Recently, a micropropagation protocol of four months duration for H. coronarium using axillary bud explants have been published (Parida et al 2013). In this study, we report a rapid (two and half month's duration) and efficient in vitro plant regeneration protocol for a valuable medicinal plant, H. coronarium using rhizome buds.…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Hedychium coronarium J. Koenig through rhizome bud

Mohanty

Behera

Swain

et al. 2013

Physiol Mol Biol Plants

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An optimized protocol was developed for in vitro plant regeneration of a medicinally important herb Hedychium coronarium J. Koenig using sprouted buds of rhizomes. The rhizomes with sprouted bud were inoculated on Murashige and Skoog (Physiol Plant 15:473-497, 1962) was most effective. In this medium, best shoots (3.6) and roots (4.0) regeneration was observed simultaneously with an average shoot and root length of 4.7 cm and 4.2 cm respectively. Regeneration of shoots and roots in the same medium at the same time (One step shoot and root regeneration) reduced the time for production of in vitro plantlets and eliminates the media cost of rooting. Cent-percent (100 %) success in plant establishment was observed in both gradual acclimatization process as well as when plants were directly transferred to outdoor in clay pots containing a mixture of garden soil and sand (2:1) without any sequential acclimatization stage.

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Section: Resultssupporting

confidence: 47%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Hedychium coronarium J. Koenig through rhizome bud

Mohanty

Behera

Swain

et al. 2013

Physiol Mol Biol Plants

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“…)Chimeric shoot tip+Israeli et al (1995)Banana cv. MartamanShoot tip−Ray et al (2006)4Brinjal ( Solanum melongena )Hypocotyl−Mallaya and Ravishankar (2013)Callus induction on leaves, nodes and intermodal explants+Naseer and Mahmood (2014)5Chrysanthemum ( Dendranthema grandiflora )Callus from leaves and internodes+Miler and Zalewska (2014)6European violet ( Viola uliginosa Besser)Leaf and petiole fragments+Slazak et al (2015)7Gerbera ( Gerbera jamesonii Bolus)Capitulum−Bhatia et al (2009, 2011)8GloxiniaLeaf explants+Hu and Xu (2010)9 Hedychium coronarium Koen.Axillary bud explants−Parida et al (2013)10Hop ( Humulus lupulus L.)Meristem tissue−Patzak (2003)11 Kaempferia galanga Buds of rhizomes−Mohanty et al (2011)12Kiwifruit ( Actinidia deliciosa (Chev.) Liang and Ferguson) cv.…”

Section: Sources Of Variations Detected In Plant Tissue Culturementioning

confidence: 99%

Somaclonal variations and their applications in horticultural crops improvement

Krishna¹,

Alizadeh

Singh³

et al. 2016

3 Biotech

332

216

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The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerated plants. However, plant tissue culture may generate genetic variability, i.e., somaclonal variations as a result of gene mutation or changes in epigenetic marks. The occurrence of subtle somaclonal variation is a drawback for both in vitro cloning as well as germplasm preservation. Therefore, it is of immense significance to assure the genetic uniformity of in vitro raised plants at an early stage. Several strategies have been followed to ascertain the genetic fidelity of the in vitro raised progenies comprising morpho-physiological, biochemical, cytological and DNA-based molecular markers approaches. Somaclonal variation can pose a serious problem in any micropropagation program, where it is highly desirable to produce true-to-type plant material. On the other hand, somaclonal variation has provided a new and alternative tool to the breeders for obtaining genetic variability relatively rapidly and without sophisticated technology in horticultural crops, which are either difficult to breed or have narrow genetic base. In the present paper, sources of variations induced during tissue culture cycle and strategies to ascertain and confirm genetic fidelity in a variety of in vitro raised plantlets and potential application of variants in horticultural crop improvement are reviewed.

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“…Such somaclonal variations occurred in P. gigantea (Samarfard et al 2014), P.' Wedding Promenade' (Lee et al 2017), and P. amabilis (Raynalta et al 2018). Meanwhile, somaclonal variation has contributed to the generation of new variants in several ornamental species, such as Saintpaulia (Matsuda et al 2014); Chrysanthemum (Miler and Zalewska 2014); gerbera (Bhatia et al 2009; and Hedicyum coronarium (Parida et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

et al. 2021

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Abstract. Putri HA, Purwito A, Sudarsono, Sukma D. 2021. Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms. Biodiversitas 22: 1077-1090. Phalaenopsis amabilis (L.) Blume is a prominent donor for the white petal and sepal trait in Phalaenopsis breeding. However, it has an undesirable character, such as susceptible to soft-rot disease. Therefore, developing soft-rot resistance mutants through gamma irradiation could be explored. This study aimed to evaluate the variability of plantlets regenerated from irradiated and non-irradiated protocorms using morphology, stomatal size and molecular markers and to test responses of the plantlets against soft-rot disease. The plantlets were regenerated from irradiated (5, 10, 15 or 20 Gy) and non-irradiated protocorms. The results showed that P. amabilis plantlet variants were successfully identified based on their leaf morphology and stomatal size variations. A few plantlets have low stomatal densities, large stomatal size, and high chloroplast numbers, which indicated they were polyploids. Leaf disc assay for soft-rot disease response grouped most of the plantlets into very susceptible or susceptible. Moreover, four soft-rot resistant plantlets regenerated from irradiated and non-irradiated protocorms were successfully identified. The resistant plantlets were identified after three consecutive periods of inoculations with pathogens causing soft-rot disease. The evaluation also confirmed nucleotide variation in the Pto gene isolated from different levels of plantlet variant resistance responses.

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In vitropropagation ofHedychium coronariumKoen. through axillary bud proliferation

Cited by 18 publications

References 34 publications

Micropropagation of Hedychium coronarium J. Koenig through rhizome bud

Micropropagation of Hedychium coronarium J. Koenig through rhizome bud

Somaclonal variations and their applications in horticultural crops improvement

Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

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