Somaclonal variations and their applications in horticultural crops improvement

Krishna, Hare; Alizadeh, M.; Singh, Dharmendra; Singh, Udayvir; Chauhan, Nitesh; Eftekhari, Mahdieh; Sadh, Radha Kishan

doi:10.1007/s13205-016-0389-7

Cited by 335 publications

(243 citation statements)

References 171 publications

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“…Molecular marker had been developed to ensure the genetic variation among varieties. Somaclonal variation can pose a serious problem in any micropropagation program, where it is highly desirable to produce true-to-type plant material (Krishna et al, 2016). One of the easiest and low cost of molecular marker is Random Amplified Polymorphic DNA (RAPD).…”

Section: Evaluation Of Callus Diversity Using Rapd Techniquementioning

confidence: 99%

In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD

et al. 2016

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Maize (Zea mays) is one of graminae plants that widely spread for many purposes wheter in food industry, feed, or bioenergy. Those high utilization required an increment in production, but unfortunately the the demands not in accordance with the volume of production since conversion of agricultural area increase lately. Indonesia has many of shoreline that recognized as marginal land where the salinity is high as well. This research try to obtain tolerant variant from two local cultivars that planted in Madura Island. Manding and Talango varieties were used as an explant for callus induction stage in MS supplemented with 2,4 D. The result showed that 4 ppm of 2,4 D were the best concentration to induce the callus in both varieties. The induced callus were exposed to medium MS that contained NaCl (0, 2500, 5000, and 7500 ppm). In 7500 ppm of NaCl, Manding variety has 100% of surviving callus, while Talango variety only 66,7%. Furthermore, Manding variety showed a better performance in callus weight improvement with 170 mg, while Talango gave no improvement in callus weight. The result of RAPD analysis indicated that the genome characteristic was different between initial callus and surviving callus. Only five primers were presence polymorphism i.e OPA 13, OPB 07, OPC 02, OPK 20, and OPU 19 from ten in total primers. Manding elected as high tolerance variety in Salinity stress, thus it proposed to be developed furthermore.

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Section: Evaluation Of Callus Diversity Using Rapd Techniquementioning

confidence: 99%

In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD

et al. 2016

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“…Stress factors including wound, exposure to sterilants, and high concentrations in plant growth regulators under culture conditions may cause genetic variation among the regenerants [20]. Therefore, assessment of genetic fidelity and biochemical constituents including antioxidant activity among regenerants with that of mother plant is imperative if pharmaceutical use and genetic transformation studies are the ultimate aim.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.

2018

J App Biol Biotech

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An efficient plant regeneration protocol through axillary shoot proliferation of nodal explants of a valuable medicinal plant, Paederia foetida, has been developed. Highest shoot multiplication was found on Murashige and Skoog's (MS) medium supplemented with 3.0 mg/l N 6 -benzylaminopurine. Upscaling of shoots was accomplished from axenic nodal explants derived from primary in vitro regenerated shoots on fresh medium of the same composition. Thus, we could obtain 71-76 shoots, in vitro, starting from a single node within 11-12 weeks. These shoots were successfully rooted on ½ MS medium and acclimatized in soil. The biochemical fidelity was assessed by evaluating the antioxidant activities of leaves of both field grown mother plant and micropropagated plants. The antioxidant activities, as carried out by 2, 2-diphenyl-1-picrylhydrazyl and metal chelating assay, of leaves collected from micropropagated plants were at par with activity of leaves collected from mother plant. Most importantly, both sources have significant antioxidant activities compared to the standard, butylated hydroxytoluene. Further, genetic fidelity of the micropropagated plants with that of the mother plant was assessed by inter simple sequence repeats markers, and their true-to-type nature was confirmed on the basis of monomorphic banding profile. This protocol has potential to create large number of clonal plants with similar antioxidant activities as the natural plants and thus could be useful for drug research and development.

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“…In vitro culture of plant cells and organs generates genetic variability ('somaclonal variation') resulting in 'somaclones' (regenerated plants that are not trueto-type) which can be used in sexual hybridisation for introgression of desirable alien genes into crop species, or to generate variants of a commercial cultivar at a high frequency without hybridizing it to other genotypes (Larkin and Scowcroft 1981). Ever since the first formal report of morphological variants in sugarcane plants produced in vitro in 1971, numerous somaclonal variations have been reported in several horticultural crops (Krishna et al 2016). Somaclonal variation manifests itself as qualitative or quantitative phenotypic changes, sequence changes, and gene activation/silencing (Kaeppler et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Often, a plant regeneration system, particularly callus-mediated plant regeneration, introduces variations that may be heritable (Krishna et al 2016). The concentration and combination of plant growth regulators (PGRs), as well as subculture frequency and duration, also result in a higher frequency of variation (Matsuda et al 2014).…”

Section: Introductionmentioning

confidence: 99%

African violet (Saintpaulia ionantha H. Wendl.): classical breeding and progress in the application of biotechnological techniques

Silva¹,

Dewir

Wicaksono

et al. 2017

Folia Horticulturae

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As a result of its domestication, breeding and subsequent commercialization, African violet (Saintpaulia ionantha H. Wendl.) has become the most famous and popular Saintpaulia species. There is interest in producing cultivars that have increased resistance to pests and low temperature, in the introduction of novel horticultural characteristics such as leaf shape, flower colour, size and form, and in improved productivity and enhanced flower duration in planta. In African violet, techniques such as the application of chemical mutagens (ethylmethanesulfonate, N-nitroso-N-methylurea), radiation (gamma (γ)-rays, X-rays, carbon ion beams) and colchicine have been successfully applied to induce mutants. Among these techniques, γ radiation and colchicine have been the most commonly applied mutagens. This review offers a short synthesis of the advances made in African violet breeding, including studies on mutation and somaclonal variation caused by physical and chemical factors, as well as transgenic strategies using Agrobacterium-mediated transformation and particle bombardment. In African violet, Agrobacterium-mediated transformation is affected by the Agrobacterium strain, selection marker, and cutting-induced wounding stress. Somaclonal variation, which arises in tissue cultures, can be problematic in maintaining true-to-type clonal material, but may be a useful tool for obtaining variation in flower colour. The only transgenic African violet plants generated to date with horticulturally useful traits are tolerant to boron (heavy metal) stress, or bear a glucanase-chitinase gene.

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Somaclonal variations and their applications in horticultural crops improvement

Cited by 335 publications

References 171 publications

In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD

In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD

In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.

African violet (Saintpaulia ionantha H. Wendl.): classical breeding and progress in the application of biotechnological techniques

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