<i>In vitro</i> production of the teleomorph of <i>Guignardia bidwellii</i>, causal agent of black rot of grapevine

Jailloux, F.

doi:10.1139/b92-035

Cited by 15 publications

(8 citation statements)

References 2 publications

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“…§Spearman's correlation coefficient. In the work of Jailloux (1992), alternating periods at 10 and 25 C resulted in the production of ascomata, and this result indirectly validates the interval 9 to 25 C used in the present work. This result was not unexpected, because Ferrin and Ramsdell (1977) and Jermini and Gessler (1996) used volumetric spore samplers, which collect spores from the air rather than from runoff water, to quantify spore release.…”

Section: Rain Variablesupporting

confidence: 86%

“…‡Pearson's correlation coefficient. In addition, the temperature interval used by Rossi et al (2014) for accumulating DD was defined based on the results of Jailloux (1992), who observed production of ascomata at a constant temperature between 5 and 15 C but not at 20 or 25 C. In the present work, the interval 9 to 25 C for accumulating DD was determined to be the best interval among a series of different intervals (not shown) based on the goodness-of-fit and the AIC (Burnham and Anderson 2002). ¶All of the correlation coefficients were significant at P < 0.001. et al (2014), however, did not provide a good fit for the cumulative number of ascospores in the runoff water from mummies in the current study (data not shown).…”

Section: Rain Variablementioning

confidence: 99%

“…This result was not unexpected, because Ferrin and Ramsdell (1977) and Jermini and Gessler (1996) used volumetric spore samplers, which collect spores from the air rather than from runoff water, to quantify spore release. In addition, the temperature interval used by Rossi et al (2014) for accumulating DD was defined based on the results of Jailloux (1992), who observed production of ascomata at a constant temperature between 5 and 15 C but not at 20 or 25 C. In the present work, the interval 9 to 25 C for accumulating DD was determined to be the best interval among a series of different intervals (not shown) based on the goodness-of-fit and the AIC (Burnham and Anderson 2002). In the work of Jailloux (1992), alternating periods at 10 and 25 C resulted in the production of ascomata, and this result indirectly validates the interval 9 to 25 C used in the present work.…”

Section: Rain Variablementioning

confidence: 99%

“…In addition, the temperature interval used by Rossi et al (2014) for accumulating DD was defined based on the results of Jailloux (1992), who observed production of ascomata at a constant temperature between 5 and 15 C but not at 20 or 25 C. In the present work, the interval 9 to 25 C for accumulating DD was determined to be the best interval among a series of different intervals (not shown) based on the goodness-of-fit and the AIC (Burnham and Anderson 2002). In the work of Jailloux (1992), alternating periods at 10 and 25 C resulted in the production of ascomata, and this result indirectly validates the interval 9 to 25 C used in the present work. Like the cumulative number of ascospores, the cumulative number of conidia in the runoff water from mummies was not well described by the logistic equation of Rossi et al (2014) with a DD base of 10 C (not shown).…”

Section: Rain Variablementioning

confidence: 99%

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Release ofGuignardia bidwelliiascospores and conidia from overwintered grape berry mummies in the vineyard

Onesti

González‐Domínguez

Manstretta

et al. 2017

Australian Journal of Grape and Wine Research

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Background and Aims Black rot, caused by Guignardia bidwellii, is a polycyclic grape disease with repeated primary and secondary infections. In this work, the release of primary inoculum (both ascospores and conidia) from overwintered grape mummies was studied over a 3‐year period. Methods and Results A spore sampler was designed consisting of: (i) a funnel for collecting spores washed off from grape mummies by rain; and (ii) microscope slides for spores being airborne in the proximity of mummies. Ascospores and conidia were frequently found in the runoff water; Gompertz equations were developed describing their seasonal cumulative numbers as a function of degree‐days, with R2 ≥ 0.92. The number of both spore types in the runoff water significantly increased as the amount of rain and its duration and intensity increased; >1 and >3 mm of rain were the best cut‐off points for predicting the release of conidia and ascospores, respectively. No ascospores were found on the microscope slides, but conidia were found on rainy days, clustered in circular areas, probably due to droplets splashing from mummies to slides during rainfall. Conclusions This work provides new information on the inoculum dynamics of G. bidwellii in vineyards that can be used in refining disease prediction models for improved disease control. Significance of the Study New equations were developed to describe the dynamics of G. bidwellii spore release as a function of degree‐days, and 1 and 3 mm of rain have been proposed as cut‐off values to predict the release of conidia and ascospores, respectively. This information could be incorporated into the mathematical models supporting the decision‐making for disease control.

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Section: Rain Variablesupporting

confidence: 86%

Section: Rain Variablementioning

confidence: 99%

Section: Rain Variablementioning

confidence: 99%

Section: Rain Variablementioning

confidence: 99%

See 2 more Smart Citations

Release ofGuignardia bidwelliiascospores and conidia from overwintered grape berry mummies in the vineyard

Onesti

González‐Domínguez

Manstretta

et al. 2017

Australian Journal of Grape and Wine Research

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“…The G. bidwellii isolate Mo05 (culture collection Geisenheim Research Center) was selected in 2004 in a commercial vineyard in the Moselle valley and cultivated on oatmeal agar (Jailloux 1992). Mycelium plugs were transferred to freshly prepared oatmeal agar dishes and thereafter cultivated for two to three weeks under 12 h light/12 h dark conditions at 20°C.…”

Section: Conidia Production and Inoculationmentioning

confidence: 99%

Protective and curative grape black rot control potential of pyraclostrobin and myclobutanil

2011

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Strobilurin or triazole type fungicides have proven to be very effective in strategies to control grape black rot (causal agent: Guignardia bidwellii) due to their combined protective and curative activities. However, differences in their long-lasting protective capacity or in the limitation of the time frame for curative treatments have not yet been clearly described regarding this pathogen. To address these issues, trials with artificial inoculation were conducted using one representative of each group, pyraclostrobin and myclobutanil on leaves of potted vines as well as on berries of field grown vines. It could be demonstrated that pyraclostrobin possesses excellent pre-infection as well as post-infection activities on both, leaves and berries. Post-bloom, pyraclostrobin was able to completely inhibit infections on berries up to three weeks after application. Full curative control of developing black rot infections was achieved for up to 85% of the total incubation period. Myclobutanil turned out to be of limited protective activity. If applied the day prior to inoculation, the efficacy level reached around 90% but decreased continuously with increasing temporal distance between application and inoculation. However, curative applications of myclobutanil in the first half of the incubation period completely prevented symptom development on leaves as well as on berries. Due to their combined reach-back and forward activity to control black rot, both, pyraclostrobin as well as myclobutanil can be recommended in practical viticulture, especially in the most susceptible period between flowering and berries beginning to touch. Pyraclostrobin offers an exceptional protective and long-lasting curative potential, whereas myclobutanil is most effective in post-infection use.

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QTL mapping of black rot (Guignardia bidwellii) resistance in the grapevine rootstock ‘Börner’ (V. riparia Gm183 × V. cinerea Arnold)

et al. 2014

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In the grapevine cultivar 'Börner' QTLs for black rot resistance were detected consistently in several independent experiments. For one QTL on chromosome 14 closely linked markers were developed and a detailed map provided. Black rot is a serious grapevine disease that causes substantial yield loss under unfavourable conditions. All traditional European grapevine cultivars are susceptible to the causative fungus Guignardia bidwellii which is native to North America. The cultivar 'Börner', an interspecific hybrid of V. riparia and V. cinerea, shows a high resistance to black rot. Therefore, a mapping population derived from the cross of the susceptible breeding line V3125 ('Schiava grossa' × 'Riesling') with 'Börner' was used to carry out QTL analysis. A resistance test was established based on potted plants which were artificially inoculated in a climate chamber with in vitro produced G. bidwellii spores. Several rating systems were developed and tested. Finally, a five class scheme was applied for scoring the level of resistance. A major QTL was detected based on a previously constructed genetic map and data from six independent resistance tests in the climate chamber and one rating of natural infections in the field. The QTL is located on linkage group 14 (Rgb1) and explained up to 21.8 % of the phenotypic variation (LOD 10.5). A second stable QTL mapped on linkage group 16 (Rgb2; LOD 4.2) and explained 8.5 % of the phenotypic variation. These two QTLs together with several minor QTLs observed on the integrated map indicate a polygenic nature of the black rot resistance in 'Börner'. A detailed genetic map is presented for the locus Rgb1 with tightly linked markers valuable for the development for marker-assisted selection for black rot resistance in grapevine breeding.

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In vitro production of the teleomorph of Guignardia bidwellii, causal agent of black rot of grapevine

Cited by 15 publications

References 2 publications

Release ofGuignardia bidwelliiascospores and conidia from overwintered grape berry mummies in the vineyard

Release ofGuignardia bidwelliiascospores and conidia from overwintered grape berry mummies in the vineyard

Protective and curative grape black rot control potential of pyraclostrobin and myclobutanil

QTL mapping of black rot (Guignardia bidwellii) resistance in the grapevine rootstock ‘Börner’ (V. riparia Gm183 × V. cinerea Arnold)

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