<i>In Vitro</i>Pollen Viability and Pollen Germination in Cherry Laurel (<i>Prunus laurocerasus</i>L.)

Sülüşoğlu, Melekber; Çavuşoğlu, Aysun

doi:10.1155/2014/657123

Cited by 69 publications

(50 citation statements)

References 21 publications

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“…Chemical tests using tetrazolium chloride and iodine potassium iodide have produced variable results in other woody plants (e.g. Sulusoglu & Cavusoglu, ). However, it is now possible to estimate pollen viability of U. glabra by its fluorochromatic reaction as a proxy (La Porta & Roselli, )—see Figure .…”

Section: Floral and Seed Charactersmentioning

confidence: 99%

Biological Flora of the British Isles: Ulmus glabra

2018

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This account presents information on all aspects of the biology of Ulmus glabraHudson (wych elm) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the Biological Flora of the British Isles: distribution, habitat, communities, responses to biotic factors, responses to environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history and conservation.2. Ulmus glabra is a large forest tree, and often an important canopy tree in ancient and semi-natural woodlands. It is primarily native to the north and west of Britain and much of mainland Europe. It is the only elm native to Ireland. It is the most distinct of the British elms in that it rarely suckers and sets abundant viable seed.Although found on limestone screes and cliffs, and hedgerows, it is primarily a woodland tree, especially on moist, basic soils. In many secondary woodlands, it often co-occurs with Acer pseudoplatanus and has ecological needs that are similar to Fraxinus excelsior. 3.Ulmus glabra has clusters of c. 25 hermaphrodite flowers appearing before the leaves on previous year's growth. Seeds are wind-dispersed, falling in April to July, but remain viable for only a few days. Nevertheless, seedling establishment can be abundant. Hybridisation with other northern European elms is common but hybrids are notoriously difficult to identify and therefore probably under-recorded.4. The health and survival of wych elm in Europe has been seriously compromised since the 1970s due to Dutch elm disease caused by the fungus Ophiostoma novoulmi, transmitted by elm bark beetles (Scolytus spp.). To the south of its Scottish stronghold, many elms are reduced to small trees regrowing from basal sprouts or seeds. These trees tend to be reinfected once trunk diameter exceeds 10 cm.Fortunately for its long-term survival, seed production usually begins a number of years before they are reinfected.

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Section: Floral and Seed Charactersmentioning

confidence: 99%

Biological Flora of the British Isles: Ulmus glabra

2018

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“…In another study, pollen grains of Okra successfully germinated in a 20% sucrose concentration but in 30% and 40% sucrose media, pollen germination and pollen tube length declined [11]. In a study of Cherry Laurel (Prunus laurocerasus L.), pollen germination rates increased at 15% sucrose and declined at 20% sucrose concentration; 10% sucrose concentration gave the highest germination percentage [9]. Staining tests with 2,3,5-triphenyl tetrazolium chloride (TTC) and iodine potassium iodide (IKI) were used to test the viability of Cherry Laurel pollen, also using in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentration for germination [9].…”

Section: Viability Test Of Pollen Collected At Anthesismentioning

confidence: 96%

“…In a study of Cherry Laurel (Prunus laurocerasus L.), pollen germination rates increased at 15% sucrose and declined at 20% sucrose concentration; 10% sucrose concentration gave the highest germination percentage [9]. Staining tests with 2,3,5-triphenyl tetrazolium chloride (TTC) and iodine potassium iodide (IKI) were used to test the viability of Cherry Laurel pollen, also using in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentration for germination [9]. Pollen was well-stained with IKI test and pollen viability estimated with TTC was better, for the best pollen germination rates was in 15% sucrose.…”

Section: Viability Test Of Pollen Collected At Anthesismentioning

confidence: 99%

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Pollen Viability of Aeschynanthus tricolor Hook.

Gandadikusumah

Wawangningrum

Rahayu

2017

JTLS

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Aeschynanthus (Gesneriaceae) is climbing or trailing epiphytes from the tropical forests of South-East Asia. Several species are widely cultivated for their attractive flowers, which usually scarlet with long corolla tubes and probably bird pollinated.This study was carried out on Aeschynanthus tricolor Hook. to determine in vitro germinability of (1) pollen collected from flowers at anthesis, on the day before anthesis, or up to five days after anthesis and (2) pollen collected on the day of anthesis and then stored at -20 o C for 127 days. Germinability tests were carried out on pollen grains after stored for four hours in 10% sucrose solution. The highest in vitro germination percentage observed was pollen collected on the day of anthesis (96.3%) and the lowest was pollen collected on the fifth day after anthesis (5.6%). The germination percentage of pollen after stored at -20 o C was 98% at beginning and 22% after 127 days. From this research we can conclude that pollen of A. tricolor can be successfully collected and then stored for some time, will be practical benefit for plant breeding and conservation purposes.

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“…Most of the media, for example, those of Prunus laurocerasus (Sulusoglu and Cavusoglu, 2014), Garcinia mangostana (Sutthinon et al, 2018), Triticum aestivum (Turan et al, 2018), and Hydrangea macrophylla, Dichroa febrifuga and their hybrids (Alexander, 2019) have been developed based on the Brewbaker and Kwack medium, which comprises 10% sucrose, 100 ppm boric acid, 300 ppm calcium nitrate, 200 ppm magnesium sulfate, and 100 ppm potassium nitrate (Brewbaker and Kwack, 1963). In fact, the very essential ones may contain only sucrose or/and boric acid (Sarkar et al, 2018); however, the presence of iron will give a better result (Brewbaker and Kwack, 1963).…”

Section: Introductionmentioning

confidence: 99%

Pollen germination and hand pollination in pitaya (Hylocereus undatus)

Li¹,

H²,

Xiao-ling³

et al. 2020

Preprint

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Hand pollination is a necessary aiding method for pitaya ( Hylocereus undatus ) production to achieve a high yield. With the cultivated area of pitaya going up exponentially in the recent years, a systematic study was carried out to understand the mechanism behind the high yield. In this study, we first developed an optimal medium for the in vitro germination of pitaya pollen. Upon testing the activity of the pollen collected from or stored for different time, we observed that the relative high pollen germination rates (27.2–65.1%) were those collected at between 2 h before blooming and 6 h after blooming, the highest activity was at 2 h after blooming, and that storing them for 24 h at 4°C reduces their germination rate from 65.2% to 35.5% and their production to about 82%. Therefore, it is not appropriate to pollinate plants with pollen that have been stored for more than 24 h, without bringing a breakthrough in pollen storage. We also observed that stigma receptivity and pollen activity are synchronous, which together determine fruit setting rate and fruit size. Pollination within 6 h after blooming provides the best fruit setting percentage and fruit size, the other favorable option being pollination at 6:00 pm, that is, 2 h before blooming; however, pollination at 6:00 am the next morning is expected to lead to 23% reduction in the production. These results will be useful for reproductive biology studies in this species. Moreover, this work set an important foundation for collecting pollen and selecting the right time of hand pollination to improve the yield and breeding efficiency in pitaya.

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In VitroPollen Viability and Pollen Germination in Cherry Laurel (Prunus laurocerasusL.)

Cited by 69 publications

References 21 publications

Biological Flora of the British Isles: Ulmus glabra

Biological Flora of the British Isles: Ulmus glabra

Pollen Viability of Aeschynanthus tricolor Hook.

Pollen germination and hand pollination in pitaya (Hylocereus undatus)

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