<i>In vitro</i> pharmacological screening of antioxidant, cytotoxic and enzyme inhibitory activities of <i>Citrus aurantifolia</i> Linn. Dried fruit extract

Karatoprak, Gökçe Şeker; Aşık, Çiğdem Yücel; Çakır, Ahmet; Şafak, Esra Köngül

doi:10.1080/09603123.2020.1714558

Cited by 12 publications

(14 citation statements)

References 25 publications

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“…Citrus aurantifolia Linn. (Rutaceae), an indigenous plant in Pakistan, has been used traditionally to treat hypertension, cough, headache, and sore throat and also as an antiseptic, anthelmintic, antiarthritic, tonic, astringent, diuretic, and appetite stimulant [14][15][16][17][18]. e fruit and other parts of the plant are being used in Ayurveda, Siddha, and many other folk systems for the cure of different ailments.…”

Section: Introductionmentioning

confidence: 99%

Antihypertensive and Vasorelaxant Effects of Citrus aurantifolia Linn. Fruit: Proposed Mechanisms

Bukhari

Alamgeer

Saeed

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Citrus aurantifolia Linn. fruit, a natural dietary item, has long been used traditionally to treat hypertension in Pakistan. The current research work aims to explore the effect on blood pressure and its mechanisms. Methods. The aqueous methanol extract of plant fruit was used to evaluate hypotensive/antihypertensive, vasorelaxation, and safety profiles. Moreover, the in vitro inhibitory effect of AMECA on phosphodiesterase was also evaluated. Results. In hypotensive studies, extracts of Citrus aurantifolia fruit exhibited a concentration-dependent reduction in SBP, DBP, MAP, and heart rate. A similar effect has been observed on anesthetized rats, but the effects exerted by the extract were not altered significantly in the presence of L-NAME, atropine, captopril, and propranolol. Moreover, in coronary arteries, the extract significantly potentiated relaxations induced by cGMP- and cAMP-dependent relaxing agonists. When exposed to PDEs, the extract concentration dependently subdued cGMP-hydrolyzing activity of different PDEs with IC50 values of 40–130 μg/mL. Conclusion. It is conceivable that extracts obtained from Citrus aurantifolia fruit produced hypotensive and antihypertensive effects in rats. The extract elicited endothelium-independent vasorelaxation, possibly by acting directly on smooth muscles of the coronary artery and by increasing cGMP and cAMP via nonselective inhibition of vascular PDEs.

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Section: Introductionmentioning

confidence: 99%

Antihypertensive and Vasorelaxant Effects of Citrus aurantifolia Linn. Fruit: Proposed Mechanisms

Bukhari

Alamgeer

Saeed

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…limonin) [27]. Fruit extract of lime has been found to reduce hyperglycemia via inhibition of pancreatic α‐amylase activity [28] and ameliorate diabetic complications [29], while its leaf essential oil decreases blood glucose level and increases hepatic glycogen concentration in diabetic rats [30]. This report attests to the ability of lime fruits in the management of diabetes and its complications, through the inhibition of polyol pathway enzymes – aldose reductase and sorbitol dehydrogenase.…”

Section: Discussionmentioning

confidence: 94%

Citrus aurantifolia(Christm.) Swingle (lime) Fruit Extract Inhibits the Activities of Polyol Pathway Enzymes

Kazeem

Bankole

Oladokun

et al. 2020

eFood

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Diabetes mellitus is associated with several complications, which may result from one or more factors including increased flux of polyol pathway. This study evaluated the inhibitory potential of four citrus fruits namely Citrus paradisi Macfad. (grape), Citrus limon (L.) Osbeck (lemon), Citrus aurantifolia (Christm.) Swingle (lime) and Citrus sinensis (L.) Osbeck (orange), on the activities of aldose reductase and sorbitol dehydrogenase -the two enzymes that mediate the polyol pathway. This is determined by incubating citrus extracts with appropriate enzymes and substrates. Fruit extract with the lowest half maximal inhibitory concentration (IC 50 ) was used to assess the mode of inhibition of the enzymes by preparing Lineweaver-Burk plot. Citrus fruits tested inhibited sorbitol dehydrogenase more than aldose reductase, which resulted in lowered IC 50 values. Of all the citrus fruits tested, lime fruit extract displayed potent inhibition of aldose reductase (IC 50 : 138.66 µg/mL) and sorbitol dehydrogenase (IC 50 : 47.21 µg/mL). Kinetic studies also revealed that lime fruit extract inhibited the activities of both enzymes in a competitive manner. In conclusion, the inhibition of the polyol pathway enzymes by the lime fruit extract may be attributed to the presence of phytochemicals such as flavonoids and limonoids.

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“…The reducing activity from iron(III) to iron(II) is an important investigation in terms of antioxidant activity evaluation and is a technique that demonstrates electron emitting capacity [ 45 ]. According to the findings of our investigation, which measured the extracts’ reducing power as ascorbic acid equivalents (AscAE), the C. pichleri subsp.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Profiles, Antioxidant, Cytotoxic, and Anti-Inflammatory Activities of Traditional Medicinal Plants: Centaurea pichleri subsp. pichleri, Conyza canadensis, and Jasminum fruticans

et al. 2022

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Centaurea pichleri subsp. pichleri, Conyza canadensis, and Jasminum fruticans are traditionally used plants grown in Turkey. Methanol extracts were obtained from these plants and pharmacological activity studies and phytochemical analyses were carried out. To evaluate the phytochemical composition, spectrophotometric and chromatographic techniques were used. The extracts were evaluated for antioxidant activity by DPPH●, ABTS●+ radical scavenging, and FRAP assays. The cytotoxic effects of the extracts were investigated on DU145 prostate cancer and A549 lung cancer cell lines. The anti-inflammatory effects of extracts were investigated on the NO amount, TNF-α, IFN-γ, and PGE 2 levels in lipopolysaccharide-stimulated Raw 264.7 cells. The richest extract in terms of phenolic compounds (98.19 ± 1.64 mgGAE/gextract) and total flavonoids (21.85 ± 0.64 mgCA/gextract) was identified as C. pichleri subsp. pichleri methanol extract. According to antioxidant activity determinations, the C. pichleri subsp. pichleri extract was found to be the most active extract. Finally, the C. pichleri subsp. pichleri methanol extract was revealed to be the most effective inhibitor of viability in the cytotoxic activity investigation, and the extract with the best anti-inflammatory action. The findings point to C. pichleri subsp. pichleri as a promising source of bioactive compounds in the transition from natural sources to industrial uses, such as new medications, cosmeceuticals, and nutraceuticals.

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In vitro pharmacological screening of antioxidant, cytotoxic and enzyme inhibitory activities of Citrus aurantifolia Linn. Dried fruit extract

Cited by 12 publications

References 25 publications

Antihypertensive and Vasorelaxant Effects of Citrus aurantifolia Linn. Fruit: Proposed Mechanisms

Antihypertensive and Vasorelaxant Effects of Citrus aurantifolia Linn. Fruit: Proposed Mechanisms

Citrus aurantifolia(Christm.) Swingle (lime) Fruit Extract Inhibits the Activities of Polyol Pathway Enzymes

Phytochemical Profiles, Antioxidant, Cytotoxic, and Anti-Inflammatory Activities of Traditional Medicinal Plants: Centaurea pichleri subsp. pichleri, Conyza canadensis, and Jasminum fruticans

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