In vitro osteogenesis on a highly bioactive glass‐ceramic (Biosilicate®)

Abstract

One of the strategies to improve the mechanical performance of bioactive glasses for load-bearing implant devices has been the development of glass-ceramic materials. The present study aimed to evaluate the effect of a highly bioactive, fully-crystallized glass-ceramic (Biosilicate) of the system P(2)O(5)-Na(2)O-CaO-SiO(2) on various key parameters of in vitro osteogenesis. Surface characterization was carried out by scanning electron microscopy and Fourier transform infrared spectroscopy. Osteogenic cells were obtained by enzymatic digestion of newborn rat calvarial bone and by growing on Biosilicate discs and on control bioactive glass surfaces (Biosilicate) parent glass and Bioglass(R) 45S5) for periods of up to 17 days. All materials developed an apatite layer in simulated body fluid for 24h. Additionally, as early as 12 h under culture conditions and in the absence of cells, all surfaces developed a layer of silica-gel that was gradually covered by amorphous calcium phosphate deposits, which remained amorphous up to 72 h. During the proliferative phase of osteogenic cultures, the majority of cells exhibited disassembly of the actin cytoskeleton, whereas reassembly of actin stress fibers took place only in areas of cell multilayering by day 5. Although no significant differences were detected in terms of total protein content and alkaline phosphatase activity at days 11 and 17, Biosilicate supported significantly larger areas of calcified matrix at day 17. The results indicate that full crystallization of bioactive glasses in a range of compositions of the system P(2)O(5)-Na(2)O-CaO-SiO(2) may promote enhancement of in vitro bone-like tissue formation in an osteogenic cell culture system.

Comparação dos efeitos do laser de baixa potência e do ultrassom de baixa intensidade associado ao Biosilicato® no processo de reparo ósseo em tíbias de ratos

“…Previous studies (both in vivo and in vitro) have demonstrated that Biosilicate® has high osteogenic potential. Moura et al (5) showed that Biosilicate® can induce bone tissue formation in a culturing system for osteoblastic cells. In an in vivo study, Granito et al (7) observed that there was greater bone formation in defects filled with Biosilicate® than in those filled with bioglass 45S5 ® , which is currently considered to be the gold standard for bioactive materials.…”

“…Among the topics studied have been the effects of treatments such as application of morphogenetic bone proteins, bioactive materials, use of low-intensity ultrasound (US) and use of lowlevel laser therapy (LLLT) (3) . Bioactive materials or biomaterials are defined as materials that are capable of producing a specific biological response at the interface between the material and the tissue, thus forming a bond between them, without being toxic or promoting immunological respon- -INTENSITY ULTRASOUND ASSOCIATED WITH BIOSILICATE® ON THE PROCESS OF BONE REPAIR IN THE RAT TIBIA ses (4,5) . In the 1990s, our research group developed a highly bioactive glass ceramic with high-performance mechanical properties, named Biosilicate® (6) .…”

Bacterial cellulose-collagen nanocomposite for bone tissue engineering

“…Therefore, the BC-COL composite could favor tissue repair for as bone tissue as soft tissues. In addition, BC membranes when functionalized with OGP or OGP [10][11][12][13][14] peptides demonstrated no in vitro cytotoxicity, genotoxicity or mutagenicity effects, and additionally the peptides influenced osteogenic cell proliferation, conferring an osteoinductive property to the BC membrane.…”

Bioactivity of leucite containing glass - ceramics using natural raw materials

“…A way to solve this problem and control degradation rate is heat treating Bioglass so as to turns it into a glass-ceramic. The latter slightly decreases the kinetics of HA formation without suppressing its bioactivity, as it has been demonstrated by many authors [1][2][3][4] . Another form to fulfill the mechanical resistant requirements is by carefully designing the composition of the precursor glass so that it develops, together with thermal treatment, crystalline phases which reinforce the material.…”