<i>In vitro</i>Organogenesis and Plantlet Formation of<i>Paulownia elongata</i>

Chang, Shujun; Donald, D. G.M.

doi:10.1080/00382167.1992.9629364

Cited by 9 publications

(6 citation statements)

References 8 publications

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“…Ratio of NAA/BAP was 1/5 in those works. The results in this study corroborated those of Rao et al (1996) and Chang and Donald (1992) indication that is the increased ratio of BAP and decreased ratio of NAA was significant to induce shoot for all explants (2, 6). Song et al (1990) noticed that ratio of shoot multiplication from P. catalpifolia meristems was enhanced by increasing concentrations of BAP up to 8.0 mg l -1 (7).…”

Section: Effects Of Explant Sources and Hormone Combinations For Reprsupporting

confidence: 88%

“…Rao et al (1996) reported that optimum shoot production from petioles of P. fortunei was obtained when MS medium supplemented with 4.5 mg.l -1 BAP and 0.8 mg.l -1 NAA was used (2). Chang and Donald (1992) used MS medium combined with 0.1 mg l -1 NAA + 0.5 mg l -1 BAP for regeneration of shoots from leaf callus of P. Elongata (6). Ratio of NAA/BAP was 1/5 in those works.…”

Section: Effects Of Explant Sources and Hormone Combinations For Reprmentioning

confidence: 99%

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Effect of Explant Source on in Vitro Propagation ofPaulownia TomentosaSteud.

Özaslan

Chen

Aytekin

2005

Biotechnology & Biotechnological Equipment

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Shoot tip, leaf with petiole, nodal stem and root explants of in vitro germinated seedlings of Paulownia tomentosa Steud. were cultured on Murashige and Skoog (MS) medium combined with 1-naphthaleneaceticacid (NAA)/6-benzylaminopurine (BAP), indole3-aceticacid (IAA)/BAP or IAA/Kinetin. Nodal stem explants were found to be an excellent explant source to induce direct ogranogenesis. The highest number of shoot regeneration from stem explants was obtained on MS medium supplemented with 0.1 mg.l-1 IAA+3.0 mg.l-1 BAP. Rooting was induced on MS basal or MS containing NAA+IAA combinations. The rooted plantlets were transferred to potting soil and adopted to greenhouse conditions.

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Section: Effects Of Explant Sources and Hormone Combinations For Reprsupporting

confidence: 88%

Section: Effects Of Explant Sources and Hormone Combinations For Reprmentioning

confidence: 99%

Effect of Explant Source on in Vitro Propagation ofPaulownia TomentosaSteud.

Özaslan

Chen

Aytekin

2005

Biotechnology & Biotechnological Equipment

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“…In vitro propagation techniques have been used for regenerating plants from various explants of Paulownia (Marcotrigiano and Stimart, 1983 [8] ; Jagannathan and Marcotrigiano, 1986 [9] ; Ni and Ma, 1982 [10] ; Chang and Donald, 1992 [11] ). To date, there has been no report of shoot production from the nodes of young seedlings of P. elongata, an economically important species in this genus.…”

Section: Bapmentioning

confidence: 99%

High Frequency Plant Regeneration from Nodal Explants of Paulownia elongata

et al. 2001

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High frequency of plant regeneration from Paulownia elongata was obtained on Murashige and Skoog (MS) medium and Woody Plant Medium (WPM), with appropriate supplements of growth regulators. Leaves, leaves with petioles, internodes and nodes excised from 3‐month‐old non‐aseptically grown P. elongata were used as explants. The highest shoot regeneration efficiency (93.7 %) was obtained from the nodes of P. elongata on MS medium supplemented with 0.1 mg/ml naphthaleneacetic acid (NAA) and 1 mg/ml 6‐benzylaminopurine (BAP). The highest root formation efficiency (100 %) from the regenerated shoots was obtained on WPM supplemented with 1 mg/ml indolebutyric acid (IBA). Rooted plantlets were transplanted to soil with a survival efficiency of almost 100 %. The regeneration system reported here could be useful for rapid multiplication of elite genotypes of P. elongata in a short period of time.

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“…Furthermore, a vegetative propagation system based on adventitious shoot production could be integrated with genetic engineering. In vitro propagation techniques have been used for several species as well as two hybrids of Paulownia (Marcotrigiano and Stimart 1983;Jagannathan and Marcotrigiano 1986;Ni and Ma 1982;Chang and Donald 1992). Although plant regeneration with direct and indirect organogenesis from various explant sources of Paulownia species have already been reported, to date there is only one report on indirect somatic embryo formation from P. tomentosa (Radojevic 1979).…”

Section: Introductionmentioning

confidence: 97%

Direct somatic embryogenesis and synthetic seed production from Paulownia elongata

2003

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We have developed a reproducible system for efficient direct somatic embryogenesis from leaf and internodal explants of Paulownia elongata. The somatic embryos obtained were subsequently encapsulated as single embryos to produce synthetic seeds. Several plant growth regulators [6-benzylaminopurine, indole-3-acetic acid, alpha-naphthaleneacetic acid, kinetin and thidiazuron (TDZ)] alone or in combination were tested for their capacity to induce somatic embryogenesis. The highest induction frequencies of somatic embryos were obtained on Murashige and Skoog (MS) medium supplemented with 3% sucrose, 0.6% Phytagel, 500 mg l(-1) casein hydrolysate and 10 mg l(-1) TDZ (medium MS10). Somatic embryos were induced from leaf (69.8%) and internode (58.5%) explants on MS10 medium after 7 days. Subsequent withdrawal of TDZ from the induction medium resulted in the maturation and growth of the embryos into plantlets on MS basal media. The maturation frequency of somatic embryos from leaf and internodal explants was 50.8% and 45.8%, respectively. Subculturing of mature embryos led to their germination on the same medium with a germination frequency of 50.1% and 29.8% from leaf and internode explants, respectively. Somatic embryos obtained directly on leaf explants were used for encapsulation in liquid MS medium containing different concentrations of sodium alginate with a 30-min exposure to 50 m M CaCl(2). A 3% sodium alginate concentration provided a uniform encapsulation of the embryos with survival and germination frequencies of 73.7% and 53.3%, respectively. Storage at 4 degrees C for 30 days or 60 days significantly reduced the survival and complete germination frequencies of both encapsulated and non-encapsulated embryos relative to those of non-stored somatic embryos. However, the survival and germination rates of encapsulated embryos increased following storage at 4 degrees C. After 30 days or 60 days of storage, the survival rates of encapsulated embryos were 67.8% and 53.5% and the germination frequencies were 43.2% and 32.4%, respectively. These systems could be useful for the rapid clonal propagation and dissemination of synthetic seed material of Paulownia elongata.

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In vitroOrganogenesis and Plantlet Formation ofPaulownia elongata

Cited by 9 publications

References 8 publications

Effect of Explant Source on in Vitro Propagation ofPaulownia TomentosaSteud.

Effect of Explant Source on in Vitro Propagation ofPaulownia TomentosaSteud.

High Frequency Plant Regeneration from Nodal Explants of Paulownia elongata

Direct somatic embryogenesis and synthetic seed production from Paulownia elongata

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