Effect of Explant Source on in Vitro Propagation of<i>Paulownia Tomentosa</i>Steud.

Özaslan, Mehmet; Chen, Can; Aytekin, Turkan

doi:10.1080/13102818.2005.10817222

Cited by 18 publications

(13 citation statements)

References 6 publications

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“…This may be due to difference in endogenous growth regulators concentration and their metabolism. Similar results were observed in Echinacea purpurea (Guda et al 2003), Tomentosa steud (Ozaslan et al 2005), and J. curcas . Genotype is one of the most important factors affecting regeneration (Feyissa et al 2005).…”

Section: Discussionsupporting

confidence: 90%

Shoot regeneration from cotyledonary leaf explants of Jatropha curcas: a biodiesel plant

2010

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A simple, high frequency, and reproducible method for plant regeneration through direct organogenesis from cotyledonary leaf explants of Jatropha curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) or 6-benzyl aminopurine (BAP). Medium containing TDZ has greater influence on regeneration as compared to BAP. The induced shoot buds were transferred to MS medium containing 10 lM kinetin (Kn), 4.5 lM BAP, and 5.5 lM a-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with different concentrations and combinations of BAP, indole-3-acetic acid (IAA), NAA, and indole-3-butyric acid (IBA). MS medium with 2.25 lM BAP and 8.5 lM IAA was found to be the best combination for shoot elongation. However, significant differences in plant regeneration and shoot elongation were observed among the genotypes studied. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing different concentrations and combinations of IBA, IAA, and NAA for 4 days, followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg l -1 activated charcoal. Elongated shoot treated with 15 lM IBA, 5.7 lM IAA, and 11 lM NAA resulted in highest percent rooting. The rooted plants could be established in soil with more than 90% survival rate. The method developed may be useful in improvement of J. curcas through genetic modification.

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Section: Discussionsupporting

confidence: 90%

Shoot regeneration from cotyledonary leaf explants of Jatropha curcas: a biodiesel plant

2010

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“…This may be due to level of endogenous hormones. Similar results were observed (Ozaslan et al 2005;Reddy et al 2008;Kumar and Reddy 2010;Kumar et al 2010a). Explant type is one of the most important factors affecting regeneration (Shen et al 2008).…”

Section: Discussionsupporting

confidence: 79%

Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants

Kumar

Anand

Reddy

2011

J. Plant Biochem. Biotechnol.

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“…This may results from differences in endogenous growth regulator concentration and their metabolism (Kaminek 1992). Similar results were observed in Tomentosa steud (Ozaslan et al 2005). The inhibitory effect of TDZ on shoot elongation has been reported by many researchers who recommend that the TDZ concentration must be reduced and/or other cytokinins or combinations of cytokinins and auxins must be used for further shoot elongation (Fasolo et al 1989;Preece and Imel 1991;Bhagwat et al 1996) and our results are in agreement with these findings.…”

Section: Discussionsupporting

confidence: 94%

In vitro plant regeneration of non-toxic Jatropha curcas L.: Direct shoot organogenesis from cotyledonary petiole explants

Kumar

Anand

Reddy

2010

J. Crop Sci. Biotechnol.

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Jatropha curcas, the energy plant has attained great attention in recent years because of its biodiesel production potential; however, oil and deoiled cakes are toxic. A non-toxic variety of J. curcas is reported from Mexico. A simple and efficient protocol has been developed for plant regeneration using cotyledonary petiole explants of non-toxic variety of J. curcas. The percentage of induction of shoot buds (59.11%), and the number of shoot buds (5.01) per explant was achieved on Murashige and Skoog's (MS) medium supplemented with 2.27 µM thidiazuron (TDZ). These induced shoot buds multiplied when subcultured on MS medium supplemented with 10 µM kinetin (Kn), 4.5 µM 6-benzyl aminopurine (BAP), and 5.5 µM -naphthaleneacetic acid (NAA) for 4 weeks and subsequent elongation achieved on MS medium supplemented with 2.25 µM BAP and 8.5 µM indole-3-acetic acid (IAA). Shoots more than 2 cm long were harvested and cultured on MS medium containing different concentrations and combinations of IBA, IAA, NAA, and 0.25 mg L -1 activated charcoal, and 19.91% rooting was achieved in 15 µM IBA, 5.7 µM IAA, and 16.5 µM NAA after 4 weeks with more than 90% survival rate.

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Effect of Explant Source on in Vitro Propagation ofPaulownia TomentosaSteud.

Cited by 18 publications

References 6 publications

Shoot regeneration from cotyledonary leaf explants of Jatropha curcas: a biodiesel plant

Shoot regeneration from cotyledonary leaf explants of Jatropha curcas: a biodiesel plant

Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants

In vitro plant regeneration of non-toxic Jatropha curcas L.: Direct shoot organogenesis from cotyledonary petiole explants

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