<i>In vitro</i>models of the human epithelial airway barrier to study the toxic potential of particulate matter

Rothen‐Rutishauser, Barbara; Blank, Fabian; Mühlfeld, Christian; Gehr, Peter

doi:10.1517/17425255.4.8.1075

Cited by 177 publications

(115 citation statements)

References 95 publications

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“…As previously highlighted, this model provides a clear basis for investigating the interaction of nano-objects with the lung. 20,26 Figure 4 shows the effects of CCNs on MDM, MDDC, and 16HBE14o-epithelial cell monocultures using the same end points as presented in relation to their effects on the triple cell coculture (Figure 3). It was observed that in each different monoculture (MDM, MDDC, and 16HBE14o-cells), the LDH release was significantly (p < 0.05) different to that found following CCN exposure to the triple cell coculture.…”

Section: ' Results and Discussionmentioning

confidence: 99%

Investigating the Interaction of Cellulose Nanofibers Derived from Cotton with a Sophisticated 3D Human Lung Cell Coculture

et al. 2011

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Section: ' Results and Discussionmentioning

confidence: 99%

Investigating the Interaction of Cellulose Nanofibers Derived from Cotton with a Sophisticated 3D Human Lung Cell Coculture

et al. 2011

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“…Risk assessment of diesel exhaust emissions was done by exposing the exhaust directly to the surface of lung cells cultured at the air-liquid interface (Steiner et al, 2012). A complex three dimensional cellular model of the human lung epithelium was used to present the lung cells (Rothen-Rutishauser et al, 2008;Muller et al, 2010). Volatile and soluble organics content are found to be higher in biodiesel exhaust compared to standard diesel (Dwivedi et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Microstructure and Chemical Composition of Diesel and Biodiesel Particle Exhaust

Popovicheva

Kireeva

Steiner

et al. 2014

Aerosol Air Qual. Res.

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The complexity and large variability of transport-emitted aerosols leads to necessity of comprehensive characterization of their physico-chemical and toxicological properties, remaining great uncertainties in health effect assessments. Particles produced by combustion of fossil diesel (B0), 20% rapeseed methyl ester in fossil diesel (B20), and pure rapeseed methyl ester (B100) were sampled from exhaust of an Opel Astra diesel engine using sulfated ash, phosphorous and sulfur (SAPS) lube oil with low and high ash content. Microscopic and chemical characterization is performed to quantify the common and specific properties of diesel/biodiesel exhausts. Hydrophobic saturated aliphatic dominate diesel particle chemistry. Oxygen and nitrogen-containing functionalities are specific for more hydrophilic biodiesel particles. A full range of chemical species for individual particles is grouped by clustering technique combined with water-soluble ion measurements. Analysis of group abundance shows carbonaceous particles (soot externally mixed with inorganic salts) and inorganic fly ash (metal oxides) in a structure of exhaust, in correlation with inorganic contaminations in fuel and lube oil. Quantification of particle types in terms of physicochemical relevance supports the identification of groups which may act as biomicromarkers discriminating between diesel and biofuel exhaust and micromarkers of using high ash lube oil, thus providing a basis for correlative toxicological assessment of diesel/biofuel engine emissions.

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“…Angemeldet Heruntergeladen am | 10.06.15 14:00 but also improved biological relevance of the system (29). Further improvements with four cell types were also described recently, showing that epithelial and endothelial cells, macrophages, and mast cells (33,34) can be cultured together to study the impact of both engineered and environmental particles.…”

Section: Bereitgestellt Von | Universitätsbibliothek Bernmentioning

confidence: 93%

“…The respiratory tract, being the most sensitive entry port of nanomaterials, has been the focus of several hundred in vitro and in vivo studies [for reviews see (28)(29)(30)]. Therefore, it is not surprising that human advanced in vitro models have been developed in order to gain more insight into the mode of action of inhalable aerosols/(nano)particles ( Figure 1A, B).…”

Section: Cell-based In Vitro Models: the Bottom Up Approachmentioning

confidence: 99%

In vitro-ex vivo model systems for nanosafety assessment

Wick

Chortarea

Guénat³

et al. 2015

European Journal of Nanomedicine

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Engineered nanomaterials have unique and novel properties enabling wide-ranging new applications in nearly all fields of research. As these new properties have raised concerns about potential adverse effects for the environment and human health, extensive efforts are underway to define reliable, cost-and time-effective, as well as mechanistic-based testing strategies to replace the current method of animal testing, which is still the most prevalent model used for the risk assessment of chemicals. Current approaches for nanomaterials follow this line. The aim of this review is to explore and qualify the relevance of new in vitro and ex vivo models in (nano) material safety assessment, a crucial prerequisite for translation into applications.

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In vitromodels of the human epithelial airway barrier to study the toxic potential of particulate matter

Cited by 177 publications

References 95 publications

Investigating the Interaction of Cellulose Nanofibers Derived from Cotton with a Sophisticated 3D Human Lung Cell Coculture

Investigating the Interaction of Cellulose Nanofibers Derived from Cotton with a Sophisticated 3D Human Lung Cell Coculture

Microstructure and Chemical Composition of Diesel and Biodiesel Particle Exhaust

In vitro-ex vivo model systems for nanosafety assessment

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