<i>In vitro</i> mineralization of <scp>MC3T3‐E1</scp> osteoblast‐like cells on collagen/nano‐hydroxyapatite scaffolds coated carbon/carbon composites

Cao, Sheng; Li, Hejun; Li, Kezhi; Lu, Jinhua; Zhang, Leilei

doi:10.1002/jbm.a.35593

Cited by 25 publications

(13 citation statements)

References 23 publications

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“…It is widely used as the scaffold during bone regeneration due to its outstanding cell adhesion, proliferation and differentiation properties. Collagen has been reported to promote MC3T3-E1 cell proliferation1718 and enhance stem cell differentiation into osteoblasts19. However, it is difficult to use alone as a biomaterial because of its low mechanic properties and easy degradation during bone tissue engineering.…”

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confidence: 99%

The mechanism of a chitosan-collagen composite film used as biomaterial support for MC3T3-E1 cell differentiation

Wang

Liu

et al. 2016

Sci Rep

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Natural composite biomaterials are good structural supports for bone cells to regenerate lost bone. Here, we report that a chitosan-collagen composite film accelerated osteoblast proliferation, differentiation and matrix mineralization in MC3T3-E1 cells. Intriguingly, we observed that the film enhanced the phosphorylation of Erk1/2. We showed that the chitosan-collagen composite film increased the transcriptional activity of Runx2, which is an important factor regulating osteoblast differentiation downstream of phosphorylated Erk1/2. Consistent with this observation, we found that the chitosan-collagen composite film increased the expression of osteoblastic marker genes, including Type I Collagen and Runx2 in MC3T3-E1 cells. We conclude that this film promoted osteoblast differentiation and matrix mineralization through an Erk1/2-activated Runx2 pathway. Our findings provide new evidence that chitosan-collagen composites are promising biomaterials for bone tissue engineering in bone defect-related diseases.

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confidence: 99%

The mechanism of a chitosan-collagen composite film used as biomaterial support for MC3T3-E1 cell differentiation

Wang

Liu

et al. 2016

Sci Rep

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“…The addition of nano-HA particles enhanced the adhesion, viability, and alkaline phosphatase activities of human mesenchymal stem cells at higher levels compared to the induction obtained by addition of micro-HA particles. In a study conducted by Cao and co-authors [39], increasing ratios of nano-HA within collagen/HA scaffolds on the surface of carbon/carbon composites had no significant impact on the proliferation activities of MC3T3 cells in vitro. However, Endo and co-workers detected decreased cell numbers with increasing amounts of octacalcium phosphate (OCP) [40] particles within the alginate/OCP microbeads.…”

Section: Discussionmentioning

confidence: 92%

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

et al. 2020

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Extensive efforts were undertaken to develop suitable biomaterials for tissue engineering (TE) applications. To facilitate clinical approval processes and ensure the success of TE applications, bioinspired concepts are currently focused on. Working on bone tissue engineering, we describe in the present study a method for biofunctionalization of collagen/hydroxyapatite composites with BMP-2 mimetic peptides. This approach is expected to be fundamentally transferable to other tissue engineering fields. A modified BMP-2 mimetic peptide containing a negatively charged poly-glutamic acid residue (E7 BMP-2 peptide) was used to bind positively charged hydroxyapatite (HA) particles by electrostatic attraction. Binding efficiency was biochemically detected to be on average 85% compared to 30% of BMP-2 peptide without E7 residue. By quartz crystal microbalance (QCM) analysis, we could demonstrate the time-dependent dissociation of the BMP-2 mimetic peptides and the stable binding of the E7 BMP-2 peptides on HA-coated quartz crystals. As shown by immunofluorescence staining, alkaline phosphatase expression is similar to that detected in jaw periosteal cells (JPCs) stimulated with the whole BMP-2 protein. Mineralization potential of JPCs in the presence of BMP-2 mimetic peptides was also shown to be at least similar or significantly higher when low peptide concentrations were used, as compared to JPCs cultured in the presence of recombinant BMP-2 controls. In the following, collagen/hydroxyapatite composite materials were prepared. By proliferation analysis, we detected a decrease in cell viability with increasing HA ratios. Therefore, we chose a collagen/hydroxyapatite ratio of 1:2, similar to the natural composition of bone. The following inclusion of E7 BMP-2 peptides within the composite material resulted in significantly elevated long-term JPC proliferation under osteogenic conditions. We conclude that our advanced approach for fast and cost-effective scaffold preparation and biofunctionalization is suitable for improved and prolonged JPC proliferation. Further studies should prove the functionality of composite scaffolds in vivo.

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“…The MC3T3 E1 cell line has been used extensively to evaluate bone templates including HAP nanocomposites and electrospun fibers . As confirmed by Wutticharoenmongkol et al, PCL nanofibers alone does not provide a sufficient microenvironment for MC3T3 E1 cells to secrete bone matrix or promote mineralization.…”

Section: Discussionmentioning

confidence: 99%

Electrospun poly(ε‐caprolactone) nanofiber shish kebabs mimic mineralized bony surface features

Gleeson

Marcolongo

2018

J Biomed Mater Res

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Electrospinning of nanofiber is of growing interest especially in bone tissue engineering because of its similar fibrous properties to the extracellular matrix. To this end, we have fabricated polycaprolactone (PCL) nanofiber shish kebab (NFSK) templates. The novelty of this work is the ability to control the mineral orientation and spatial location on the nanofiber, mimicking natural collagen fibers. However, NFSK templates have properties that need to be investigated in terms of cellular response including fiber alignment and crystallization. In this study, MC3T3 E1 preosteoblast cells were seeded onto the templates to determine the effect of both fiber orientation and kebab size on the cell metabolic activity. PCL was electrospun to form aligned and randomly oriented nanofibers, which were then crystallized in a PCL solution in pentyl acetate for 15 and 60 min, resulting in the formation of homopolymer PCL NFSK templates. We evaluated the cell proliferation and alkaline phosphatase activity of MC3T3 E1 cells after 3, 7, and 14 days in coculture. Aligned nanofiber and polymer crystallization both significantly increased the cell proliferation and alkaline phosphatase activity at each time point. The aligned nanofibers and polymer crystallization resulted in the highest metabolic activities of the cells compared to the randomly oriented fibers and noncrystallized controls.

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In vitro mineralization of MC3T3‐E1 osteoblast‐like cells on collagen/nano‐hydroxyapatite scaffolds coated carbon/carbon composites

Cited by 25 publications

References 23 publications

The mechanism of a chitosan-collagen composite film used as biomaterial support for MC3T3-E1 cell differentiation

The mechanism of a chitosan-collagen composite film used as biomaterial support for MC3T3-E1 cell differentiation

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

Electrospun poly(ε‐caprolactone) nanofiber shish kebabs mimic mineralized bony surface features

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