<i>In Vitro</i>Micrografting of the Almond Cultivars “Texas”, “Ferrastar” and “Nonpareil”

Yıldırım, Hakan; Akdemir, Hülya; Süzerer, Veysel; Çiftçi, Yelda Özden; Onay, Ahmet

doi:10.5504/bbeq.2012.0087

Cited by 13 publications

(5 citation statements)

References 29 publications

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“…New root development was not observed in male micrografts when proliferation medium was used, but 20% of female micrografts developed further roots. Several researchers reported that the composition of the medium was one of the important factors for micrografting success of almond cultivars (Fotopoulos and Sotiropoulos, 2005;Yıldırım et al, 2010Yıldırım et al, , 2013 and of pistachio (Onay et al, 2003a(Onay et al, , 2004. Similarly, in this study significant differences were reported in the mean rates of micrografting success, as well as in axillary shoot and root development in both genotypes for the three types of media tested.…”

Section: Discussionsupporting

confidence: 69%

“…On account of the results of previously reported micrografting methods concerning almond (Yıldırım et al, 2013) and pistachio (Onay et al, 2004), slit micrografting was used in this study. Three different culture media were tested for optimum micrograft development: 1) MS medium containing 30 g/L sucrose and 6.7 g/L agar; 2) shoot proliferation MS medium containing 30 g/L sucrose, 1.0 mg/L BA, and 7 g/L agar; 3) rooting MS medium containing 30 g/L sucrose, 1.0 mg/L indole-3-butyric acid (IBA), and 6.7 g/L agar.…”

Section: Effects Of Medium Typementioning

confidence: 99%

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Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability

Onay¹,

Tilkat²,

Süzerer³

et al. 2016

Turk J Biol

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IntroductionMastic trees are commonly known as evergreen pistachio or lentisk. In Turkey lentisk shares its habitat with Pistacia terebinthus L., olives, carob, and others, but it prefers welldrained to dry sandy or stony alkaline soil conditions (Ak and Parlakcı, 2009). Mastic trees show a peculiar adaptability to several climatic conditions (Zohary, 1952) and have positive biological characteristics, such as drought tolerance (Correia and Catarino, 1994) and protection of soil against erosion (Mulas and Deidda, 1998). However, the area that is suitable for mastic tree cultivation is limited in the world. Today a major limitation facing the widespread expansion of commercial mastic tree plantations is the shortage of superior plants, primarily because of the difficulties experienced in propagating this species using the traditional method of rooting the cuttings. Currently mastic trees are proliferated by seed, with the rational development of hereditary instability and great discrepancy in the formation degree among genotypes because of obstacles such as parthenocarpy and ovary aborticide (Grundwag, 1976). Vegetative propagation by cutting is also difficult due to impoverished or adventitious roots. Conventional propagation methods to improve Pistacia species such as P. lentiscus L. are limited because species belonging to the genus Pistacia have a long stage of juvenility. In addition, plants originating from seeds often do not maintain the genetic purity of the parent plants due to segregation and recombination of genetic characteristics during sexual reproduction. Thus, to improve trade, a vegetative propagation technique must be developed for large-scale propagation. Possible ways to move forward are necessary in order to avoid problems in vegetative propagation and to produce clonal stocks. Micropropagation of lentisk genotypes has been achievedAbstract: An in vitro propagation method was established for both male and female genotypes of lentisk using actively growing shoot tips derived from forcefully lignified shoots. The effects of growth regulators on in vitro morphogenesis were investigated. Since rooting of the regenerated shoots for both genotypes was not achieved, an in vitro micrografting method was developed for the production of plantlets. Moreover, genetic stability of 3-, 6-, and 24-times subcultured clones of both genotypes was assessed and compared with the mother plants using fluorescent-based amplified fragment length polymorphism (AFLP) analysis. The set of main plants and the different subcultured clones were divided into two clusters. In the first cluster, the original male and female plants were grouped together with the 3-times subcultured female and the 6-times subcultured male and female groups, whereas the second cluster contained the 24-times subcultured clones and the 3-times subcultured male group. To the best of our knowledge, this is the first report of successfully inducing plantlets from mature lentisk genotypes and the first analysis of clonal fidelity of regenerated mature...

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Section: Discussionsupporting

confidence: 69%

Section: Effects Of Medium Typementioning

confidence: 99%

Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability

Onay¹,

Tilkat²,

Süzerer³

et al. 2016

Turk J Biol

Self Cite

View full text Add to dashboard Cite

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“…Micrografting involves in vitro placing of shoot tip as an explant on a decapitated rootstock grown from a seed ( Hussain et al, 2014 ). Micrografting protocols have been developed for many fruit crops including grapes ( Aazami and Bagher, 2010 ), walnut ( Wang et al, 2010 ), almond ( Yıldırım et al, 2013 ), etc. Rafail and Mosleh (2010) reported that in in vitro grafting of apple and pear, homografting was relatively more successful compared to heterografting and an increase in micrograft success was noticed from 30 to 90% in pear (cv.…”

Section: Grafting Techniques In Fruit Treesmentioning

confidence: 99%

Mechanisms Underlying Graft Union Formation and Rootstock Scion Interaction in Horticultural Plants

et al. 2020

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Grafting is a common practice for vegetative propagation and trait improvement in horticultural plants. A general prerequisite for successful grafting and long term survival of grafted plants is taxonomic proximity between the root stock and scion. For the success of a grafting operation, rootstock and scion should essentially be closely related. Interaction between the rootstock and scion involves complex physiological-biochemical and molecular mechanisms. Successful graft union formation involves a series of steps viz., lining up of vascular cambium, generation of a wound healing response, callus bridge formation, followed by vascular cambium formation and subsequent formation of the secondary xylem and phloem. For grafted trees compatibility between the rootstock/scion is the most essential factor for their better performance and longevity. Graft incompatibility occurs on account of a number of factors including of unfavorable physiological responses across the graft union, transmission of virus or phytoplasma and anatomical deformities of vascular tissue at the graft junction. In order to avoid the incompatibility problems, it is important to predict the same at an early stage. Phytohormones, especially auxins regulate key events in graft union formation between the rootstock and scion, while others function to facilitate the signaling pathways. Transport of macro as well as micro molecules across long distances results in phenotypic variation shown by grafted plants, therefore grafting can be used to determine the pattern and rate of recurrence of this transport. A better understanding of rootstock scion interactions, endogenous growth substances, soil or climatic factors needs to be studied, which would facilitate efficient selection and use of rootstocks in the future. Protein, hormones, mRNA and small RNA transport across the junction is currently emerging as an important mechanism which controls the stock/scion communication and simultaneously may play a crucial role in understanding the physiology of grafting more precisely. This review provides an understanding of the physiological, biochemical and molecular basis underlying grafting with special reference to horticultural plants.

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“…Another drawback of the research conducted on micrografting processes refers to the use of seedling rootstocks. In fruit trees micrografting most authors used seedling rootstocks produced in vivo: Abousalim and Mantell [30] and Onay et al [31] with pistachio, Fengtong et al [32] with mulberry or in vitro, Yildirim et al [33,34] with almond, Naz et al [35] with citrus, and Hassanen [36] with pear.…”

Section: Discussionmentioning

confidence: 99%

Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (Juglans hindsi × Juglans regia) cl. ‘Vlach’

Ribeiro

Pires

et al. 2022

Agronomy

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In vitro micropropagation is already a current multiplication tool for walnut self-rooted cultivars and rootstocks, but walnut grafting is still performed in the field or in greenhouses, mainly using seedlings as rootstocks. The present work describes a new approach to obtain clonal walnut-grafted plants, involving in vitro shoot production of ‘Paradox’ (Juglans hindsi × Juglans regia) cl. ’Vlach’, to be used as rootstock, and J. regia cv. ‘Chandler’, to be used as scion. After completing the in vitro multiplication phase and a seven-day root induction treatment, ‘Vlach’ explants are transferred to ex vitro conditions for root expression while being simultaneously grafted using the in vitro produced ‘Chandler’ scions. The importance of the presence of leaves on both the scion and the rootstock for the success rate of the technique was evaluated. Under optimal conditions, average success rates of 82% for rootstock rooting, 72% for micrografting survival, and 84% for grafted plant acclimatization were achieved. This rooting/grafting combination technique seems able to compete with the traditional techniques of nursery grafting, allowing obtaining high-quality walnut-grafted plants independently of the external weather conditions in a significantly shorter time.

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In VitroMicrografting of the Almond Cultivars “Texas”, “Ferrastar” and “Nonpareil”

Cited by 13 publications

References 29 publications

Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability

Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability

Mechanisms Underlying Graft Union Formation and Rootstock Scion Interaction in Horticultural Plants

Ex Vitro Rooting and Simultaneous Micrografting of the Walnut Hybrid Rootstock ‘Paradox’ (Juglans hindsi × Juglans regia) cl. ‘Vlach’

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