<i>In vitro</i> micrografting of apical and axillary buds of cacao

Miguelez-Sierra, Yulien; Hernández-Rodríguez, Annia; Acebo-Guerrero, Yanelis; Baucher, Marie; Jaziri, Mondher El

doi:10.1080/14620316.2016.1215231

Cited by 12 publications

(11 citation statements)

References 18 publications

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“…This stable in vitro environment and the probable pathogen-free status of micro-scion/rootstock may favor callus formation and rapid establishment of vascular reconnection between scions and rootstocks required for grafting success [34,35]. Micrografting protocols have been developed for many fruit crops including almond [18], apple [36], apricot [23], avocado [37], cacao [38], cashew [39], cherimoya [19], cherry [21], citrus [40,41], guava [4], grape [34], jujube [42], mulberry [43], hazelnut [44], kiwifruit [25], passion fruit [26], olive [45], peach [46], pear [47], pistachio [17], plum [48], walnut [49,50], and watermelon [51].…”

Section: Overall Developments and Characters Of Micrograftingmentioning

confidence: 99%

“…To prepare rootstock seedlings for micrografting, in vitro germination of seeds is the first step, with varied protocols needed to promote germination. Miguelez-Sierra et al [38] found that cacao seeds did not require any pretreatment for the effective in vitro seed germination; in their protocol, seeds were taken from mature pods, were surface sterilized, inoculated in culture medium, and three-week-old seedlings were used as rootstocks. However, removal of the seed coat is necessary in many cases of in vitro germination.…”

Section: Preparation Of Rootstocksmentioning

confidence: 99%

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In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

et al. 2022

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In vitro micrografting is an important technique supporting the micropropagation of a range of plant species, particularly woody plant species. Over the past several decades, in vitro micrografting has become a strategy to facilitate shoot recovery and acclimatization of in vitro-grown horticultural species. This review focuses on studies on horticultural crops over the past two decades that cover the establishment of in vitro micrografting, discusses factors affecting the success of in vitro micrografting, and provides commentary on the contribution of micrografting applications to the field of micropropagation. Considering the important roles of micrografting in the restoration of vigor and rooting competence, in promotion of shoot recovery following somatic embryogenesis and organogenesis, and in facilitation of shoot regrowth after cryopreservation, the potential use of this technique in facilitation of genetic engineering and safe conservation of horticultural species are specially highlighted.

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Section: Overall Developments and Characters Of Micrograftingmentioning

confidence: 99%

Section: Preparation Of Rootstocksmentioning

confidence: 99%

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

et al. 2022

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“…Dari lapisan kambium akan terbentuk jaringan pembuluh sehingga proses translokasi hara dari batang bawah ke batang atas atau sebaliknya hasil fotosintesis dari batang atas ke batang bawah berlangsung sebagaimana mestinya Miguelez-Sierra et al (2017).…”

Section: Penelitian Ini Dilakukan DI Laboratoriumunclassified

Pengaruh Pembelahan Eksplan Biji Dan Jenis Media Terhadap Keberhasilan Sambung Mikro Manggis Di Pesemaian

Agustina

Maisura

Hafifah

et al. 2020

JAT

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Teknik sambung mikro dapat dilakukan di pesemaian dengan kondisi tanaman yang sama seperti pada sambung mikro in vitro. Keunggulan sambung mikro in vitro eksplan biji utuh adalah tingkat keberhasilan dan pertumbuhan tanamannya lebih baik dibandingkan tunas dari perkecambahan biji dibelah. Penelitian ini dilakukan di Laboratorium Kultur Jaringan Tanaman dan Kebun Percobaan Fakultas Pertanian Universitas Malikussaleh. Penelitian ini dilaksanakan dari bulan September 2018 sampai dengan Juni 2019. Tujuan penelitian ini adalah untuk mengetahui pengaruh pembelahan biji untuk sumber batang bawah dan jenis media terhadap keberhasilan sambung mikro manggis di pesemaian. Percobaan ini menggunakan Rancangan Acak Kelompok (RAK) dua faktor. Faktor pertama adalah sumber batang bawah terdiri dari 2 taraf yaitu tunas dari pesemaian biji utuh dan tunas dari pesemaian biji dibelah 2, Faktor kedua adalah jenis media tanam yaitu media tanam tanah dan media tanam pasir dengan 10 ulangan. Hasil sidik ragam menunjukkan bahwa tidak ada pengaruh interaksi antara perlakuan pembelahan biji manggis dan media tanam terhadap semua peubah yang diamati. Perlakuan pembelahan biji manggis berpengaruh terhadap waktu tumbuh tunas dan panjang tunas sambung mikro manggis di pesemaian. Perlakuan jenis media hanya berpengaruh terhadap peubah panjang tunas 7 dan 8 MSP. Perlakuan biji utuh memberikan pengaruh lebih baik daripada biji dibelah dua dan Media tanah memberikan pengaruh lebih baik daripada media pasir.

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“…To address these challenges, the development of an efficient in vitro grafting system, which includes younger tissues under controlled environmental conditions in the growth chamber, seems to be potential and has successfully been utilized in different types of fruit species (Cardoso et al, 2012;Miguelez-Sierra et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro grafting of Psidium guajava in Psidium cattleianum for the Management of the Meloidogyne enterolobii

Macan

Cardoso

2019

International Journal of Fruit Science

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Guava is one of the most important tropical fruit crops; however, its cultivation has been affected by the prevalence of the nematode Meloidogyne enterolobii, which leads to a large-scale reduction in the production of fruits and restricts new plantations in the contaminated areas. The discovery of the resistance of Psidium cattleianum to this nematode has increased the possibility of its selective use as rootstock for guava; a few studies have worked on the graft; however, the rootstock exhibits a high incompatibility with the commercial varieties of guava. Therefore, the present study aimed to develop an in vitro-grafting technique of guava scions under rootstocks of P. cattleianum obtained using an in vitro seed germination method. The successful in vitro-grafting was performed using semisolid MS1/2 (Murashige and Skoog) culture medium with 0.5 mg L −1 of Benzyladenine. P. cattleianum with approximately 3 cm length was used as rootstocks, and apical guava stems with 0.5 cm length were used as scions. The grafting procedure used was wedge grafting. To increase the success rate of in vitro-grafting, we used a sterile paper moistened with sterilized distilled water to prevent dehydration. The use of MS1/2 and the wedge grafting resulted in the overall success rate of 30% for the graft between P. cattleianum and Psidium guajava. In conclusion, this is the first report of successful in vitro-grafting between guava and P. cattleianum.

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In vitro micrografting of apical and axillary buds of cacao

Cited by 12 publications

References 18 publications

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

Pengaruh Pembelahan Eksplan Biji Dan Jenis Media Terhadap Keberhasilan Sambung Mikro Manggis Di Pesemaian

In vitro grafting of Psidium guajava in Psidium cattleianum for the Management of the Meloidogyne enterolobii

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