In vitroinvestigations of a novel wound dressing concept based on biodegradable polyurethane

Abstract

Non-healing and partially healing wounds are an important problem not only for the patient but also for the public health care system. Current treatment solutions are far from optimal regarding the chosen material properties as well as price and source. Biodegradable polyurethane (PUR) scaffolds have shown great promise for in vivo tissue engineering approaches, but accomplishment of the goal of scaffold degradation and new tissue formation developing in parallel has not been observed so far in skin wound repair. In this study, the mechanical properties and degradation behavior as well as the biocompatibility of a low-cost synthetic, pathogen-free, biocompatible and biodegradable extracellular matrix mimicking a PUR scaffold was evaluated in vitro. The novel PUR scaffolds were found to meet all the requirements for optimal scaffolds and wound dressings. These three-dimensional scaffolds are soft, highly porous, and form-stable and can be easily cut into any shape desired. All the material formulations investigated were found to be nontoxic. One formulation was able to be defined that supported both good fibroblast cell attachment and cell proliferation to colonize the scaffold. Tunable biodegradation velocity of the materials could be observed, and the results additionally indicated that calcium plays a crucial role in PUR degradation. Our results suggest that the PUR materials evaluated in this study are promising candidates for next-generation wound treatment systems and support the concept of using foam scaffolds for improved in vivo tissue engineering and regeneration.

3D-Printed Gelatin Methacrylate Scaffolds with Controlled Architecture and Stiffness Modulate the Fibroblast Phenotype towards Dermal Regeneration

“…Swelling is also beneficial in the wound-healing process as it builds a small amount of pressure on the wound boundaries [ 55 ]. This may increase surface contact with this tissue, contributing to decreased wound contraction and, as a result, stimulating cell ingrowth and therefore wound healing [ 55 , 56 ]. Due to the changes in the porous architecture across the design range, the swelling ability of the 3D-printed GelMA scaffolds was investigated and found to be independent of pore size or porosity ( Figure 6 ).…”

Production of Biodegradable Palm Oil-Based Polyurethane as Potential Biomaterial for Biomedical Applications

“…The cytotoxicity test was conducted by the extract method [ 34 ] whereby the medium used in incubating the polyurethane samples was used for MTT assay. Polyurethane samples were first cut into 5 × 5 × 4 mm 3 rectangular shape and sterilized using ultraviolet in a biosafety cabinet for 30 min [ 35 ].…”

One‐pot synthesis of palm oil‐based polyester polyol for production of biodegradable and biocompatible polyurethane

“…The cytotoxicity of the polyurethane was evaluated through the extract method by using human osteosarcoma MG 63 cells. Briefly, polyurethane was cut into rectangular shape (5 × 5 × 4 mm 3 ) and sterilized under ultraviolet on each side of the polyurethane for 30 min .…”

“…Briefly, polyurethane was cut into rectangular shape (5 × 5 × 4 mm 3 ) and sterilized under ultraviolet on each side of the polyurethane for 30 min . After sterilization, the polyurethane samples were immersed into 2 mL of DMEM containing 10% FBS and 1% penicillin/streptomycin and incubated for 1, 5, and 10 days at 37 °C in a CO 2 incubator . The extracts collected were used in MTT assay.…”