<i>In vitro</i> induction and differentiation of newborn guinea pig hippocampus neural stem cells into cells resembling inner hair cells, using artificial perilymph

Wang, Y; Mm, Dong

doi:10.1017/s0022215111000922

Cited by 2 publications

(2 citation statements)

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“…In both studies, first MSCs differentiated into neural precursor cells and then differentiated into hair cells by defined medium. Wang & Dong (2011) isolated neural stem cells from newborn guinea-pig hippocampus and differentiated them into hair cells both with defined medium and artificial perilymph techniques.…”

Section: Discussionmentioning

confidence: 99%

Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells

et al. 2017

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SUMMARY:Surgical techniques for treatment of sensory neural hearing loss (SNHL) have unpredictable outcomes and in recent years cell therapy investigated for treatment of SNHL. Olfactory epithelium proceed neurogenesis during life time and provide an easily accessible source of neural stem cells. So the aim of this study was isolating neural stem cells from olfactory epithelium of rat and differentiation of these cells into hair cells of inner ear in vitro. The epithelium tissue of olfactory mucosa of rats were removed and digested by collagenase H. The digested tissue was cultured in flasks in suspension forms to create spheres. Spheres were passaged and from passage 2 spheres selected for differentiation. At this stage cells of spheres isolated from each other and placed in flask containing defined differentiation medium. Cells at this stage cultured in adhesive form. Immunohistochemistry and RT-PCR were used for neural stem cells and hair cells identification. Spheres formed from olfactory epithelium culture and immunohistochemistry revealed that cells of spheres from passage one and two expressed the neural stem cells markers. After culture of isolated cells in differentiation medium, the morphology of cells begun to change. The cells presented neural cells projections and after 10 days the projections elongated more and interact to each other in multi layers. RT-PCR and immunohistochemistry revealed that differentiated cells expressed hair cells specific genes. In this study we showed that neural stem cells of olfactory epithelium can differentiate into hair cells of inner ear and therefore can be used for treatment of SNHL.

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Section: Discussionmentioning

confidence: 99%

Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells

et al. 2017

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“…Recently Chen, et al [ 23 ] reported the restoration of auditory evoked responses using embryonic stem cell derived otic porgenitors. Other neural SCs may be more applicable for SGN replacement [ 9 12 14 24 ]. The auditory epithelium, HC-like cells or SCs which have a potential to differentiate into HCs may be used, although questions still exist whether these cells are enough to replace damaged HCs and other cells in the auditory epithelium including various types of supporting cells (e.g., Deiter's, Pilla, Claudius, Hensen's etc.).…”

Section: Reviewmentioning

confidence: 99%

Stem Cell Therapy for Sensorineural Hearing Loss, Still Alive?

Park

2015

J Audiol Otol

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In mammals, the auditory system, which includes the cochlea, has a very complex structure harboring many types of cells performing different functions. Among these cells are the auditory hair cells (HCs), which are terminally and well differentiated unique cells which have lost their regenerative potential after development. The auditory HCs are easily damaged by aging as well as during episodes of ototoxicity and acoustic trauma. HCs damages typically occur in the early stage of injury and can result a permanent hearing loss. Recently, there have been tremendous developments from stem cells (SCs) research involving sensorineural hearing loss, but several limitations and obstacles persist in allowing these developments from continuing onto clinical applications. This review discusses the recent advances in SC research in sensorineural hearing loss with the subsequent sections discussing the possible hurdles and limitations that currently preclude their clinical application.

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In vitro induction and differentiation of newborn guinea pig hippocampus neural stem cells into cells resembling inner hair cells, using artificial perilymph

Abstract: Neural stem cells can survive in both fetal bovine serum and artificial perilymph, and within these media can differentiate into cells with hair-cell-specific antibodies. This provides an experimental basis for transplantation of neural stem cells into the inner ear.

Cited by 2 publications

References 7 publications

Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells

Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells

Stem Cell Therapy for Sensorineural Hearing Loss, Still Alive?

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