In vitro immunomodulatory activity of flavonoid glycosides from Urtica dioica L.

Akbay, Pınar; Başaran, Arif Ahmet; Ündeğer, Ülkü; Başaran, Nurşen

doi:10.1002/ptr.1068

Cited by 190 publications

(93 citation statements)

References 24 publications

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“…The chemical constituents of aqueous nettle leaf extracts are diverse and over 100 compounds have been described including polyphenols (eugenol, flavones), phytosterols (cholecalciferol, carnosol) and flavonoids such as quercitin and Kaempherol [15,25,26). Carnosol has demonstrated anti-proliferative activity against LNCaP cells [27] and more interestingly the compounds quercitin and kaempferol have been shown to induce apoptotic death of LNCaP cells via PARP cleavage and DNA fragmentation [28] and could be responsible for the cytotoxic activities seen in the current study.…”

Section: Discussionmentioning

confidence: 99%

Urtica dioica Induces Cytotoxicity in Human Prostate Carcinoma LNCaP Cells: Involvement of Oxidative Stress, Mitochondrial Depolarization and Apoptosis

Levy¹,

Sivanesan²,

Ramalingam³

et al. 2014

Trop. J. Pharm Res

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Section: Discussionmentioning

confidence: 99%

Urtica dioica Induces Cytotoxicity in Human Prostate Carcinoma LNCaP Cells: Involvement of Oxidative Stress, Mitochondrial Depolarization and Apoptosis

Levy¹,

Sivanesan²,

Ramalingam³

et al. 2014

Trop. J. Pharm Res

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“…Alcalóides (ROBERTS et al, 1982, BUCK et al, 1986AKBAY et al, 2003) e saponinas (SONODA et al, 1998) isolados de extratos de plantas medicinais têm demonstrado capacidade de interferir nos mecanismos responsáveis pela locomoção de leucócitos. As plantas do gênero Rauwolfia são fontes ricas de alcalóides, sendo que vários deles já foram identificados (Batista et al, 1996), particularmente para a R. sellowii, Müll.…”

Section: Discussionunclassified

Influência de extratos hidroetanólicos de plantas medicinais sobre a quimiotaxia de leucócitos humanos

Presibella¹,

Santos²,

Weffort-Santos³

2003

Rev. bras. farmacogn.

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RESUMO:Vários métodos in vitro têm sido empregados para a investigação das atividades biológicas de plantas usadas na medicina popular para o tratamento de processos inflamatórios. Neste trabalho, investigou-se a influência dos extratos hidroetanólicos de Rauvolfia sellowii Muell. Arg, Hybanthus bigibbosus (St.-Hil) Hassler e Anchieta pyrifolia (Mart.) G. Don, conhecidas popularmente como pau-pra-tudo, canela-de-veado e cipó-suma, respectivamente, sobre a quimiotaxia de leucócitos humanos, estimulados a migrar contra um gradiente de caseína, utilizando-se o método de Boyden. A dexametasona foi utilizada como substância de referência da inibição da quimiotaxia leucocitária. Os resultados demonstraram efeito inibitório significativo de todos os extratos das plantas testadas, sobre a migração de polimorfonucleares, induzida por caseína. Entretanto, essa atividade variou de intensidade conforme a concentração e a espécie estudada. Efeitos máximos foram observados, nas concentrações de 1000, 10 e 1µg/ml com os extratos de pau-pra-tudo, canela-de-veado e cipó-suma, respectivamente, com migração de 81,6±3,9%; 85,4±2,4% e 91,7±2,2% dos polimorfonucleares, enquanto que, com a dexametasona, este efeito foi de 70,3±5,9%. Embora estudos mais aprofundados sejam necessários, os resultados apresentados podem servir como base preliminar de dados, contribuindo para esclarecer o mecanismo da atividade antiinflamatória atribuída às essas plantas na medicina caseira. Unitermos: Rauvolfia sellowii; Hybanthus bigibbosus; Anchieta pyrifolia; plantas medicinais; migração leucocitária; caseína; inflamação; quimiotaxia. ABSTRACT: Influence of some medicinal plant hydroethanolic extracts on human leukocyte chemotaxis. Several in vitro methods have been used for the investigation of the biological activities of plants used in folk medicine for the treatment of inflammatory conditions. In this study, we have investigated the ability of the hydroethanolic extracts from Rauvolfia sellowii Muell. Arg, Hybanthus bigibbosus (St.-Hil) Hassler, and Anchieta pyrifolia (Mart.) G. Don, locally known as pau-pra-tudo, canela-de-veado, and cipó-suma, respectively, in interfering with the human leukocytes migration induced by casein, using the Boyden chamber method. Dexamethasone has been used as a positive control for leukocyte inhibition in the same experimental approach. The data herein presented showed a significant inhibition of the casein-induced polymorphonuclear leukocytes migration for all plants studied. However, the intensity of such activity was variable according to the dose and plant tested. For pau-pra-tudo, canela-de-veado, and cipó-suma extracts the average number of migrated polymorphonuclear leukocytes was 81.6±3.9%, 85.4±2.4% and 91.7±2.2% of the input for the doses of 1000, 10, and 1mg/ml, respectively, while for dexamethasone, the value found was 70.3±5.9%. Although further studies are needed, the results presented in this study may be useful to clarify the anti-inflammatory properties of these herbal medicines, supporting their eth...

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“…(Akbay, et al, 2003) to determine the quercetin-like flavonoids present in the extract-loaded microspheres. A Beer's calibration curve for quercetin was employed as the standard (Chang, et al, 2002).…”

Section: W W (%) Yieldmentioning

confidence: 99%

Some Physical Properties of Vernonia amygdalina and Garcinia kola Microspheres Prepared with High Molecular Weight Polyethylene Glycols

Eraga¹,

Arhewoh²,

Ivuongbe³

et al. 2015

Journal of Applied Sciences and Environmental Management

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ABSTRACT:The effect of polymer concentrations on some of the physicochemical properties of Vernonia amygdalina (Linn) and Garcinia kola (Heckel) extracts loaded microspheres was evaluated. Microspheres of the aqueous extracts was prepared by emulsion solvent evaporation using polyethylene glycol (PEG) mixtures of molecular weight 4000 and 6000 at different ratios of 1:0, 0:1, 1:1, 1:2 and 2:1 while the amounts of the extracts incorporated was constant for all ratios. The microspheres were evaluated for their particles sizes, yield, flavonoid content, loading efficiency, moisture loss and flow properties. In-vitro release studies were carried out by monitoring flavonoid release rate from the microspheres. The microspheres were spherical and uniformly shaped and exhibited good flow characteristics. Their size range, yield, loading efficiency, moisture loss and flavonoid content were 76 -83 μm, 49 -76 %, 47 -82 %, 2.18 -4.60 % and 17.10 -23.80 mg%, respectively for V. amygdalina and 144 -160 μm, 50 -68 %, 51 -68 %, 3.00 -4.41 % and 20.00 -28.70 mg%, respectively for G. kola. Flavonoids release from the microsphere was up to 90 % within 1 h and it followed a matrix release kinetic model with a super case-II transport mechanism. The concentrations of the polymers affected the yield, loading efficiency, moisture loss and the extent of flavonoid release of the microspheres but had no effect on their particle sizes and flavonoid content. These results may find useful application in the delivery of V. amygdalina and G. kola extracts since the combination of PEG of different molecular weights resulted in microspheres with good physicochemical and release properties. © JASEM http://dx

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In vitro immunomodulatory activity of flavonoid glycosides from Urtica dioica L.

Cited by 190 publications

References 24 publications

Urtica dioica Induces Cytotoxicity in Human Prostate Carcinoma LNCaP Cells: Involvement of Oxidative Stress, Mitochondrial Depolarization and Apoptosis

Urtica dioica Induces Cytotoxicity in Human Prostate Carcinoma LNCaP Cells: Involvement of Oxidative Stress, Mitochondrial Depolarization and Apoptosis

Influência de extratos hidroetanólicos de plantas medicinais sobre a quimiotaxia de leucócitos humanos

Some Physical Properties of <i>Vernonia amygdalina</i> and <i>Garcinia kola</i> Microspheres Prepared with High Molecular Weight Polyethylene Glycols

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