In Vitro Evaluation of Proliferation and Migration Behaviour of Human Bone Marrow-Derived Mesenchymal Stem Cells in Presence of Platelet-Rich Plasma

Abstract

Objective. To access the effects of platelet-rich plasma (PRP) on the behaviour of human bone marrow-derived mesenchymal stem cells (hBMSCs), including proliferation and migration. Methods. PRP was diluted with DMEM/F12, resulting in concentrations of 1%, 2%, and 5%. The proliferation of hBMSCs was examined by 2 methods: cell-number counting with the haemocytometer method and the colony-forming unit-fibroblast (CFU-F) assay. Cell migration was evaluated using the scratch wound healing (SWH) assay; after that, the recorded digital images were analysed by the Image-Analysis J 1.51j8 software to compare the cell-free areas between groups after 0, 24, and 48 hours. Results. hBMSCs cultured in DMEM/F12 at PRP concentrations of 1%, 2%, and 5% were all able to proliferate and migrate. In the 5% PRP group, hBMSCs proliferated greatly with a significantly higher cell number than reported for all other groups on days 5, 7, and 9. CFU-Fs were observed in all groups, except for the negative control group. The SWH assay demonstrated that hBMSCs cultured in 2% and 5% PRP almost filled the artificial wound scratch and significantly migrated more than those of all other groups at both 24 h and 48 h. Conclusion. This study indicated that, due to the significant enhancement of cell proliferation and migration, 5% PRP might be the optimal concentration that should be used to promote the potential of hBMSCs in wound healing.

Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations

“…As an alternative to these approaches, the use of PRP as clinical-grade adjuvant to enhance the therapeutic effectiveness of engrafted MSC has been suggested by several studies highlighting that PRP treatment improves the angiogenic potential of MSC both in vitro and in vivo [29, 30] and stimulates the proliferation of MSC in vitro [31, 32]. However, systematic studies on whether PRP alters the repair properties of engrafted MSC in skin wound healing are lacking.…”

The in vitro effects of platelet products on the biophysiological functions of human bone marrow mesenchymal stromal cells: a systematic review

“…Only 6 of these 21 studies had a sample size of n ≥ 10 for both components (PPs and BM-MSCs) (Ben Azouna et al, 2012;Jenhani et al, 2011;Lucarelli et al, 2003;Moisley et al, 2019;Verrier et al, 2010;Yin et al, 2016); with the remaining having a small sample size of n ≤ 6 for one or both components. Discrepancy in sample size between PPs and BM-MSCs within the same study was also observed (Amable et al, 2014;Ben Azouna et al, 2012;Bernardi et al, 2017;Bernardi et al, 2013;Goedecke et al, 2011;Gottipamula et al, 2012;Infante et al, 2017;Jenhani et al, 2011;Kasten et al, 2008;Parsons et al, 2008;Perut et al, 2013;Prins et al, 2009;Vogel et al, 2006;Yin et al, 2016) (Table 2a,b and 3a,b).…”

“…The effect of incubation time on PPs was only assessed by 1 study (Parsons et al, 2008), whereby BM-MSCs incubated with 2.5 % PRC showed more proliferation in the first 24 h only, with proliferation equal to that of the control group at 48 h and inferior to control group at 72 h.…”

“…The chemotactic effects of PP on the migratory capacity of BM-MSCs was only assessed by 8 studies (Table 9). PRP-CM, PR, PRF-CM and PG-CM demonstrated positive chemotactic effects on the migratory capacity of BM-MSC in vitro (Gruber et al, 2004;Nguyen et al, 2019;Schar et al, 2015;Yin et al, 2016). Most PL studies reported on its positive chemotactic effects (Infante et al, 2017;Moisley et al, 2019;Murphy et al, 2012).…”

“…PRP-CM, PR, PRF-CM and PG-CM demonstrated positive chemotactic effects on the migratory capacity of BM-MSC in vitro (Gruber et al, 2004;Nguyen et al, 2019;Schar et al, 2015;Yin et al, 2016). Most PL studies reported on its positive chemotactic effects (Infante et al, 2017;Moisley et al, 2019;Murphy et al, 2012). Migratory capacity of BM-MSCs improved with increasing PP concentration (up to 10 %) in most studies that compared between different PP concentrations [PL: n = 2 (Goedecke et al, 2011;Murphy et al, 2012) and PR: n = 2 (Gruber et al, 2004;Nguyen et al, 2019)].…”