In vitro evaluation of natural hydroxyapatite from Osteoglossum bicirrhosum fish scales for biomedical application

Abstract

This study reports the obtainment of bioactive hydroxyapatite (HAp) extracted from scales of arowana fish (FSHA) (Osteoglossum bicirrhosum) by alkaline treatment followed by calcination at 600 and 800°C. The cell viability and bioactivity of hydroxyapatite particles (FSHA) were investigated and compared with those of HAp synthesized (s.HA) by the precipitation method. The HAp particles from fish scales showed non‐toxic behavior to dental pulp stem cells similar to HAp synthesized. Additionally, bioactivity assays show that the Hap from natural source forms the bone‐like apatite layer faster than s.HA sample, after being incubated in McCoy medium for 3 days. The results illustrate that HAp obtained from Osteoglossum bicirrhosum fish scale bio‐waste showed excellent biocompatibility. Besides, this study provides an effective method for converting low‐cost bio‐waste into a value‐added and it can be a potential alternative biomaterial for various biomedical applications.

Hydroxyapatite from biowaste for biomedical applications: obtainment, characterization and in vitro assays

“…The FB-HAp800 sample presented after 7 days of immersion, which can be considered the beginning of the formation of the apatite layer through the formation of a filamentous structure on the particles. A similar result was reported by Horta et al 14 for 3 days of immersion in McCoy medium for hydroxyapatite extracted from Osteoglossum bicirrhosum fish scales. It can be stated that the time used in the study was not enough for the growth of the layer for the C-Hap and E-HAp samples.…”

“…Hydroxyapatite can be obtained by chemical reactions using different reagents that contain the calcium and phosphate ions, using synthetic or natural calcium sources, such as hen's eggshells [6][7][8][9] , shells, corals 10,11 , etc. Hydroxyapatite can also be extracted from biogenic sources, such as bovine bones, pig bones 12 , fish bones and scales 13,14 . Hen's eggshells, fish bones and scales become interesting source of calcium since they are abundant and accessible, reducing the costs of raw materials and bringing environmental benefits with the use of these residues 15,16 .…”

Microestrutura, Densificação E Microdureza De Pastilhas De Hidróxiapatita Obtida a Partir De Escama De Peixe Prensadas E Sinterizadas

“…Pastilhas prensadas e sinterizadas de HAp foram produzidas em duas condições: amostras de pó de HAp que não passou por tratamento térmico (Verde) e pó de HAp tratado termicamente a 1000°C por 5h. HAp foi obtida a partir de escamas de Pirarucu, seguindo rota de síntese como apresentado em Horta et. al, 2021 As amostras foram prensadas em uma prensa hidráulica uniaxial da PUCRio, utilizando uma carga de 5 toneladas durante 30 segundos.…”

Microestrutura, Densificação E Microdureza De Pastilhas De Hidróxiapatita Obtida a Partir De Escama De Peixe Prensadas E Sinterizadas

“…Diferentes tipos de peixes vem sendo utilizados em pesquisas para produção de Hidroxiapatita a partir de escamas. Horta et. al, 2021e Silva et.…”