In vitro evaluation of anticancer effects of different probiotic strains on HCT‐116 cell line

Abstract

Aim Since the evolution of man, microbes are associated with humans, playing a vital role in the maintenance of good health. However, an imbalance in the gut microbial ecosystem is associated with several diseases including colorectal cancer (CRC). The supplementation with probiotics has been proven to be beneficial in improving CRC. In this study, we have evaluated the anticancer effects of 11 probiotic strains on human colorectal carcinoma cell line (HCT‐116). Methods and Results In this study, HCT‐116 cells were treated with various concentrations (0·5, 5, 10, 20 and 200 million CFU per ml) of probiotic strains. The viability was analysed using a MTT assay and IC50 values were determined. Besides this, we evaluated the expression of multiple genes involved in the apoptosis and stress tolerance by real‐time PCR. Lactobacillus reuteri (UBLRu‐87), Saccharomyces boulardii (Unique‐28), Bacillus clausii (UBBC‐07), Bacillus coagulans (Unique‐IS2), Streptococcus salivarius (UBSS‐01), Lactobacillus fermentum (UBLF‐31), Lactobacillus salivarius (UBLS‐22), Bifidobacterium bifidum (UBBB‐55) and Lactobacillus plantarum (UBLP‐40) exhibited potent cytotoxicity on HCT 116 cells. Furthermore, UBLF‐31 and Unique‐28 induced the expression of CJUN, CFOS and CASP‐9, and downregulated the expression of BCL6. UBLRu‐87 and UBBB‐55 induced the expression of CJUN, CFOS and CASP‐9 but not BCL‐6. UBLP‐40, UBBC‐07, UBLS‐22, and Unique‐IS2 induced the expression of CJUN and CASP‐9 and downregulated the expression of BCL‐6. Conclusion These studies indicate the anticancer effects of selected probiotic strains by inducing apoptosis. Significance and Impact of the Study The probiotic strains with the anticancer effects identified in this study can be proposed as potential candidates in the treatment of CRCs.

Balancing reactive oxygen species generation by rebooting gut microbiota

“…Furthermore, the administration of LGG to children with acute gastroenteritis increased a non‐specific humoral immune response by an enhancement in IgG, IgA and IgM secretion from the circulating lymphocytes (Gómez‐Llorente et al, 2010). In addition, a recent study by Yenuganti and co‐workers proposed the use of probiotic strains ( Alkalihalobacillus clausii (formerly known as Bacillus clausii ) (UBBC‐07), Bifidobacterium bifidum (UBBB‐55), Bacillus coagulans (Unique‐IS2), Ligilactobacillus salivarius (formerly known as Lactobacillus salivarius ) (UBLS‐22), L. reuteri (UBLRu‐87), L. fermentum (UBLF‐31), L. plantarum (UBLP‐40), Saccharomyces boulardii (Unique‐28) and Streptococcus salivarius (UBSS‐01)) in CRCs treatment due to their cytotoxic effect on human colorectal carcinoma cell line (HCT‐116) by inducing apoptosis genes (BCL6, CASP9, CFOS and CJUN) (Yenuganti et al, 2021).…”

Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges

“…Furthermore, recent studies suggest that L. salivarius strains represent an emerging option for cancer prevention and treatment (Zhang et al 2013;Zhang et al 2015;Wang et al 2016b;Yenuganti et al 2021).…”

Probiotics as Potential Biological Immunomodulators in the Management of Oral Lichen Planus: What’s New?

“…Taken together, these results indicate that S. cerevisiae may be involved in CRC progression, by acting on cell apoptosis [ 188 ]. Different probiotic strains, including S. boulardii (Unique-28), B. bifidum (UBBB-55), L. reuteri (UBLRu-87), L. plantarum (UBLP-40), L. fermentum (UBLF-31), L. salivarius (UBLS-22), Bacillus clausii (UBBC-07), Bacillus coagulans (Unique-IS2) and S. salivarius (UBSS-01), were put in contact with HCT116 cells for 24 h. Cancer cell apoptosis was induced again, confirming the possible use of selected probiotics as coadjutants for CRC [ 189 ].…”