In vitro effects of beetroot juice and chips on oxidative metabolism and apoptosis in neutrophils from obese individuals

Abstract

Oxidative stress and inflammation are involved in the development of obesity. Beetroot (Beta vulgaris var. rubra) is a food ingredient containing betalain pigments that show antioxidant activity. The in vitro effect of beetroot juice and chips on oxidative metabolism and apoptosis in neutrophils from obese individuals has been investigated. Fifteen obese women (aged 45 ± ± ± ± ± 9 years, BMI > > > > >30 kg/m 2 ) and nine healthy controls (women, aged 29 ± ± ± ± ± 11 years, BMI = = = = = 22.2 ± ± ± ± ± 1.6 kg/m 2 ) were examined. The investigated products were used as concentrates and after transport and digestion in an artificial gastrointestinal tract. Neutrophil oxidant production, in response to phorbol 12-myristate 13-acetate, was characterized by luminol-dependent chemiluminescence and a flow cytometric dichlorofluorescin oxidation assay. Caspase-3 activity, a marker of apoptosis, was measured by cleavage of the fluorogenic substrate Ac-DEVD-AMC. Neutrophils from obese individuals had a significantly higher ROS production compared with the controls ( p < < < < < 0.05). Beetroot products inhibited neutrophil oxidative metabolism in a concentration-dependent manner. Also observed were the pro-apoptotic effects of beetroot at a concentration range of 0.1-10% in 24 h culture of stimulated neutrophils. These natural products (in both the liquid and solid state) have antioxidant and antiinflammatory capacity, and could be an important adjunct in the treatment of obesity.

Chokeberry(Aronia melanocarpa)– A Review on the Characteristic Components and Potential Health Effects

“…These findings go in line with reports showing an effect of other procyanidin-rich foods on diabetes [108], [109], [110]. Recent human studies also document that Aronia juice may be useful in in the treatment of obesity disorders [111].…”

Comparison of in vitro anti-lipase and antioxidant activities, and composition of commercial chokeberry juices

“…Furthermore, the levels of total phenolics (2.73-10.35 g/l) and total anthocyanins (0.10-0.67 g/l) estimated in this study agree with the literature data. Total phenolic content determined by the Folin-Ciocalteu method varied from 5.46 [31] to 18.54 g/l [32], while total anthocyanin content estimated by pH-differential method ranged from 0.15 [11] to 3.04 g/l [6]. The large differentiation noticed in both the present and previous studies may be due to differences in chokeberry cultivars, maturation stages as well as technology of juice production, and time of the juice storage.…”

“…Results of in vitro and in vivo studies indicate that chokeberry juice has cardioprotective [7,8], hepatoprotective [9], and anti-diabetic effects [1] as well as anticancer activity [10]. Recent human studies also showed that Aronia melanocarpa juice may be useful in the treatment of obesity disorders [11]. Black chokeberry constituents may help in reducing dietary fat absorption by inhibiting digestive lipases activity.…”

In vitro antioxidant, anti-diabetic and antilipemic potentials of quercetagetin extracted from marigold (Tagetes erecta L.) inflorescence residues

“…One of the major causes of the metabolic syndrome is excessive consumption of carbohydrates and triglycerides. Triglycerides can cause abnormal accumulation of body fat since it has the highest energy density (9 kcal/g) (Zielinska-Przyjemska et al 2009). One therapeutic approaches is to retard carbohydrates and lipids absorption by inhibiting the activity of the key digestive enzymes in the digestive system.…”