<i>In Vitro</i>Development of Ciprofloxacin Resistance of<i>Salmonella enterica</i>Serovars Typhimurium, Enteritidis, and Indiana Isolates from Food Animals

Zhang, Wenhui; Zhang, Chuan-Zhen; Liu, Zhijie; Gu, Xianfeng; Wan, Li; Yang, Ling; Liu, Yahong; Zeng, Zhenling; Jiang, Hongxia

doi:10.1089/mdr.2016.0119

Cited by 10 publications

(13 citation statements)

References 36 publications

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“…Here, we observed a high prevalence of the ParC T57S substitution in strains with lowest MICs of ciprofloxacin (S and RS strains). Whether the ParC T57S substitution contributes to quinolone resistance, is still controversial ( Baucheron et al, 2002 ; Ling et al, 2003 ; Eaves et al, 2004 ; Gunell et al, 2009 ; Zhang W.H. et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Contribution of Different Mechanisms to Ciprofloxacin Resistance in Salmonella spp.

et al. 2021

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Development of fluoroquinolone resistance can involve several mechanisms that include chromosomal mutations in genes (gyrAB and parCE) encoding the target bacterial topoisomerase enzymes, increased expression of the AcrAB-TolC efflux system, and acquisition of transmissible quinolone-resistance genes. In this study, 176 Salmonella isolates from animals with a broad range of ciprofloxacin MICs were collected to analyze the contribution of these different mechanisms to different phenotypes. All isolates were classified according to their ciprofloxacin susceptibility pattern into five groups as follows: highly resistant (HR), resistant (R), intermediate (I), reduced susceptibility (RS), and susceptible (S). We found that the ParC T57S substitution was common in strains exhibiting lowest MICs of ciprofloxacin while increased MICs depended on the type of GyrA mutation. The ParC T57S substitution appeared to incur little cost to bacterial fitness on its own. The presence of PMQR genes represented an route for resistance development in the absence of target-site mutations. Switching of the plasmid-mediated quinolone resistance (PMQR) gene location from a plasmid to the chromosome was observed and resulted in decreased ciprofloxacin susceptibility; this also correlated with increased fitness and a stable resistance phenotype. The overexpression of AcrAB-TolC played an important role in isolates with small decreases in susceptibility and expression was upregulated by MarA more often than by RamA. This study increases our understanding of the relative importance of several resistance mechanisms in the development of fluoroquinolone resistance in Salmonella from the food chain.

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Section: Discussionmentioning

confidence: 99%

Contribution of Different Mechanisms to Ciprofloxacin Resistance in Salmonella spp.

et al. 2021

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“…Interestingly, the only exception was the most distant isolate relative to the other isolates from Brazil that was assigned ST4632 based on a single SNP in one of the MLST genes. Nearly all isolates exhibited fluoroquinolone resistance (98.4%), a worrying observation as these antibiotics are considered the first-line antimicrobial agents for the treatment of salmonellosis [30–32]. In 2016, the EFSA also reported a substantial number (64.7%) of ciprofloxacin-resistant isolates (breakpoint MIC of >0.064 μg/ml, EUCAST) from broiler meat in 16 of the 19 European Member States that participated.…”

Section: Discussionmentioning

confidence: 99%

Characterization and whole genome sequencing of closely related multidrug-resistant Salmonella enterica serovar Heidelberg isolates from imported poultry meat in the Netherlands

et al. 2019

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Multidrug-resistant Salmonella enterica serovar Heidelberg isolates are frequently recovered in the Netherlands from poultry meat imported from South America. Our aim was to retrospectively assess the characteristics of the antimicrobial determinants, gene content and the clonal relatedness of 122 unique S . Heidelberg isolates from chicken meat from Brazil (n = 119) and Argentina (n = 3) that were imported between 2010 and 2015. These isolates were subjected to antimicrobial susceptibility testing, PCR and Illumina HiSeq2500 whole genome sequencing. Draft genomes were assembled to assess the gene content, and the phylogenetic relationships between isolates were determined using single nucleotide polymorphisms. Ciprofloxacin-resistance was identified in 98.4% of the isolates and 83.7% isolates showed resistance to the extended-spectrum cephalosporins cefotaxime and ceftazidime (83.6% and 82.8% respectively). Of the latter, 97.1% exhibited an AmpC phenotype and contained bla CMY-2 , whereas the remaining three isolates contained an extended spectrum beta-lactamase. Of the 99 extended-spectrum cephalosporins-resistant isolates harboring CMY-2 plasmids, 56.6% contained the incompatibility group I1 replicon. Phylogenetic cluster analysis showed that all isolates from Brazil clustered together, with 49% occurring in clusters larger than 5 isolates that revealed intra-cluster similarities based on geographical location and/or resistance profiles. The remaining isolates were classified in smaller clusters or as singletons, highlighting the large diversity of S . Heidelberg in the poultry chain in Brazil that was revealed by this study. Considering the potential public health risk associated with multidrug-resistant S . Heidelberg in imported poultry, collaborative whole genome sequencing-based surveillance is needed to monitor the spread, pathogenic properties and epidemiological distribution of these isolates.

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“…The fluoroquinolone resistance in Gram-negative bacteria is primarily the result of mutations in the chromosomal gene encoding the quinolone targets gyrA , gyrB , parC and parE . Single mutations in the gyrA gene confers low level quinolone resistance while high-level fluoroquinolone resistance from gyrA mutations often requires an additional single or double mutation in parB or parC [43, 44]. However, decreased susceptibility to fluoroquinolones in C .…”

Section: Discussionmentioning

confidence: 99%

The first isolation of Clostridium difficile RT078/ST11 from pigs in China

Zhang

Yang

et al. 2019

PLoS ONE

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We investigated the molecular characteristics and antimicrobial susceptibility of Clostridium difficile isolated from animals in China. We obtained 538 rectal swabs from pigs, chickens and ducks in 5 provinces during 2015 and 2016. C . difficile isolates were characterized by detection of toxin genes, multilocus sequence typing and ribotyping. And antimicrobial susceptibility testing was performed using the agar dilution method. Out of 538 samples, 44 (8.2%) were C . difficile positive with high prevalence in pigs (n = 31). Among these, 39 (88.6%) were toxigenic including 14 (31.8%) that were A + B + CDT + and 13 (29.5%) A + B + . The remaining 12 (27.3%) were A - B + . We identified 7 ST types and 6 PCR ribotypes. The most predominant type was ST11/RT078 with toxin profile A + B + CDT + and all were isolated from piglets with diarrhea. ST109 isolates possessed two different toxigenic profiles (A - B - CDT - and A - B + CDT - ) and although it was not the most prevalent sequence type, but it was widely distributed between chickens, ducks and pigs in the 5 provinces. All C . difficile isolates were fully susceptible to vancomycin, metronidazole, fidaxomicin, amoxicillin/clavulanate and meropenem but retained resistance to 4 or 5 of the remaining antibiotics, especially cefotaxime, tetracycline, ciprofloxacin, cefoxitin. The RT078/ST11 isolates were simultaneously resistant to cefotaxime, tetracycline, cefoxitin, ciprofloxacin and imipenem. This is the first report of the molecular epidemiology of C . difficile isolated from food animals in China. We identified the epidemic strain RT078/ST11 as the predominate isolate among the animals we screened in our study.

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In VitroDevelopment of Ciprofloxacin Resistance ofSalmonella entericaSerovars Typhimurium, Enteritidis, and Indiana Isolates from Food Animals

Cited by 10 publications

References 36 publications

Contribution of Different Mechanisms to Ciprofloxacin Resistance in Salmonella spp.

Contribution of Different Mechanisms to Ciprofloxacin Resistance in Salmonella spp.

Characterization and whole genome sequencing of closely related multidrug-resistant Salmonella enterica serovar Heidelberg isolates from imported poultry meat in the Netherlands

The first isolation of Clostridium difficile RT078/ST11 from pigs in China

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