In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer (Mazama gouazoubira) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer

Assisted Reproductive Technology in Neotropical Deer: A Model Approach to Preserving Genetic Diversity

“…A similar pattern of expression was observed between reconstructed goat/goat and deer/cattle embryos. For SCNT that used cattle cytoplasts (cattle/cattle and deer/cattle), the pattern of gene expression was similar to that of bovine embryos produced by IVEP for all the genes after expression normalization of the mitochondrial activity, which was performed by mitochondria using the 16S gene [124].…”

Extranuclear Inheritance of Mitochondrial Genome and Epigenetic Reprogrammability of Chromosomal Telomeres in Somatic Cell Cloning of Mammals

“…Different possible patterns/scenarios of extranuclear (cytoplasmic) inheritance of mtDNA fractions have been presented (see Figure 1 (for intraspecies cloning by SCNT) [2,158,165,172], Figure 2 (for interspecies SCNT using nuclear donor cells and recipient oocytes derived from closely related mammalian species) [163,170,171,173,174], and Figure 3 (for interspecies SCNT using nuclear donor cells and recipient oocytes derived from phylogenetically distant mammalian species) [162,164,175,176]).…”

Ex Situ Conservation and Genetic Rescue of Endangered Polish Cattle and Pig Breeds with the Aid of Modern Reproductive Biotechnology – A Review

“…To sum up, the efficiency of intra-species and inter-species somatic cell cloning of domesticated mammals is affected, to a great extent, by three factors: the provenance of frozen/thawed and ex vivo-expanded nuclear donor cell lines (NDCLs) that provide genetic resources from different livestock species or breeds (Samiec and Skrzyszowska, 2010;Li et al, 2013;Olivera et al, 2016;Lee et al, 2019), the morphological, cytogenetic, cytophysiological and molecular quality parameters of the NDCLs (Samiec et al, 2013(Samiec et al, , 2019Zhang et al, 2019;Wiater et al, 2021); and the subsequent incidence of programmed (apoptotic or autophagic) cell death in the development of cloned embryos (Chi et al, 2017;Samiec et al 2015;Jeong et al, 2020). This efficiency is also dependent on such molecular mechanisms as the epigenetic ability of the nuclear donor cell-inherited genome to be reprogrammed in SCNT-derived oocytes and result in cloned embryos (Samiec and Skrzyszowska, 2018;Deng et al, 2020;Sampaio et al, 2020;Wang et al, 2020b) and the intergenomic crosstalk between nuclear and mitochondrial DNA fractions in the host cytoplasm of nuclear recipient oocytes and the blastomeres of the developing cloned embryos (Srirattana et al, 2011;Samiec and Skrzyszowska, 2021;Takeda, 2019;Xu et al, 2019;Magalhães et al, 2020) (Figure 1).…”

“…for somatic cell banking and intra-species somatic cell cloning have a large application potential for the Polish economy, science and interdisciplinary research. These strategies could lead to the conservation of genetic resources and establishment of the genetic reserves of endangered or vanishing native breeds, restoration and multiplication of subpopulations of disappearing and rare conservative livestock breeds to maintain biodiversity genetic variation, and the reintroduction into the anthropogenic agricultural ecosystem niches breeds that are the ancestors of some primitive breeds characterized by persistent resistance to adverse environmental and climatic conditions (Wells et al, 1998;Arat et al, 2011;Selokar et al, 2014;Magalhães et al, 2020). Moreover, practically applied research into the cloning of different livestock breeds could serve to achieve other tangible benefits, including improved genetics (fertility and prolificacy) and production (increased milk and meat yield).…”