<i>In-vitro</i>cytotoxic screening of<i>Glycyrrhiza glabra</i>L. (fabaceae): A natural anticancer drug

Sg, Rathi; Suthar, Maulik P.; Patel, Parag R.; Vh, Bhaskar; Rajgor, Nirali

doi:10.4103/0975-1483.57071

Cited by 23 publications

(14 citation statements)

References 8 publications

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“…The reported percentage of licorice extract cytotoxic activity was 63% at 0.24 mg/mL with greater percentages apparent as the concentrations of licorice were increased up to 4.8 mg/mL. Rathi et al (66) reported that IC 50 values of chloroform, methanol, and water extracts of G. glabra L. in the breast MCF7 cell line were 0.45, 0.99, and 1.29 µM, respectively. In the present study, the G. glabra water extract displayed mutagenic activity against the TA100 strain in the presence of metabolic activation, but only at the highest extract concentration.…”

Section: Discussionmentioning

confidence: 99%

Toxic potentials of ten herbs commonly used for aphrodisiac effect in Turkey

Asadi¹,

Nath²,

Özhan³

2015

Turk J Med Sci

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IntroductionSince ancient times, herbs have played an important role in the treatment of different diseases in many regions of world, largely for their expectorant, aphrodisiac, diuretic, diaphoretic, antispasmodic, stomachic, and sedative properties (1). The World Health Organization reported that 70%-80% of the global population relies on herbs for primary health care. In addition, because they are natural products, it is generally believed that herbs are essentially safe and free from side effects. Consequently, the consumption of nonprescription herbs has steadily increased over the past few decades (2).Despite the therapeutic advantages of herbs, reports have indicated the potential toxicity of some herbal components. Herbs contain a variety of chemical substances that act upon the body. In addition, the quality of herbs can be affected by toxic contaminants originating from industry, agriculture, and private households, including toxins that can cause mutagenic and carcinogenic effects from long-term and widespread use (3-6). Moreover, herbal medicines are generally a mixture of a number of herbs in a single preparation and contain several active ingredients that induce various pharmacological effects (7,8). To date, there are few scientific studies on the safety and potential toxicity of herbs, despite growing concerns over the lack of both scientific evidence and quality control data regarding the safety and efficacy of herbs (9,10).Sexual dysfunction is a condition that affects 15-30 million men worldwide, and it occurs in 10%-52% of men and 25%-63% of women (11,12). Many believe that sexual dysfunction is associated with the modern lifestyle. As a result, individuals turn to natural products, such as traditional herbs, that produce aphrodisiac effects for sexual enhancement. Aphrodisiac herbs are used to treat sexual dysfunction because they alter specific neurotransmitters or sex hormones. Specifically, herbs with aphrodisiac properties can induce vasodilatation that results in a sustained erection, and can cause irritation of genital mucosa that enhances sensory experience during coitus (13).Background/aim: Sexual dysfunction is a serious problem worldwide. In Turkey, herbal products are used by some people suffering from sexual dysfunction. Despite their therapeutic advantages, some constituents of herbs are potentially toxic and pose health risks because they can be bought from the market without a prescription. Therefore, we aimed to determine the safety of herbs possessing aphrodisiac effects, chosen on the basis of their frequency of medicinal use and commercial importance in Turkey. Materials and methods:Ten herbs (Anethum graveolens, Carthamus tinctorius, Citrus aurantium, Cocos nucifera, Glycyrrhiza glabra, Melissa officinalis, Nigella arvensis, Pinus pinea, Prunus mahaleb, and Zingiber officinale) were extracted with water, methanol, and chloroform. The cyto-and genotoxic potentials of the extracts were assessed using an MTT test on a rat kidney cell line and an Ames assay in Salmonella typhi...

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Section: Discussionmentioning

confidence: 99%

Toxic potentials of ten herbs commonly used for aphrodisiac effect in Turkey

Asadi¹,

Nath²,

Özhan³

2015

Turk J Med Sci

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“…Previous studies with Fabaceae species have shown that the Gymnocladus dioicus extract exhibits a cytotoxic effect on HeLa cells in a concentration-dependent manner (Jantova et al, 2001). Bremner et al (2009) reported that Ononis ramosissima extract exhibits a cytotoxic effect on HeLa cells, while Rathi et al (2009) demonstrated that Glycyrrhiza glabra extract exhibits a cytotoxic effect on human breast cancer (MCF-7) cells. (Demir et al, 2019b).…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

Aliyazıcıoğlu

Demir

Yaman

et al. 2019

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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The purpose of this study was to investigate the phytochemical analysis of D. pentaphyllum and its cytotoxic activity on human cervical cancer (HeLa) cells with possible mechanisms. Total phenolic contents (TPC) and phytochemical analysis of the extract were evaluated using spectrophotometric method and RP-HPLC, respectively. The cytotoxic effect of the extract was evaluated using the MTT assay. The mechanism of the cytotoxic effect of the extract was also evaluated in terms of apoptosis, cell cycle, and mitochondrial membrane potential (MMP) using the fluorometric methods. The TPC of the extract was calculated as 68.9±2.3 mg gallic acid equivalent per g sample and quercetin was determined as the major phenolic in the extract. The extract showed a selective cytotoxic effect (6.5-fold) on HeLa cells compared to normal fibroblast cells. The cytotoxic effect of the extract was found to be due to the arresting of the cell cycle in the S phase and increasing apoptosis through decreased MMP. Further studies are needed for the comprehensive effects of the extract on cell signaling.

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“…[32] The utility of cell lines acquired from tumor allows the investigation of tumor cells in a simplified and controlled environment. [33] Common basic steps of in vitro cytotoxic screening include: (a) Isolation of cells, (b) incubation of cells with drugs, (c) assessment measure of cell viability. [33] It is now well-documented that apoptosis or programmed cell death is the key mechanism by which chemotherapeutic agents exert their cytotoxicity.…”

Section: Resultsmentioning

confidence: 99%

“…[33] Common basic steps of in vitro cytotoxic screening include: (a) Isolation of cells, (b) incubation of cells with drugs, (c) assessment measure of cell viability. [33] It is now well-documented that apoptosis or programmed cell death is the key mechanism by which chemotherapeutic agents exert their cytotoxicity. [34] Cytotoxicity has been defined as the cell killing property of a chemical compound independent from the mechanism of death.…”

Section: Resultsmentioning

confidence: 99%

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Untitled

Jain

2017

AJP

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Background: The goal of the present investigation was to evaluate docetaxel (DOC)-loaded gelatin nanoparticles for cancer therapy. Materials and Methods: DOC-loaded gelatin nanoparticles using ultraviolet-visible spectroscopy, X-ray diffraction, particle size and size distribution, scanning electron microscopy, drug entrapment efficiency, infrared, and in vitro drug release were characterized. The viability of MCF-7 breast cancer (BC) cells was determined by MTT. Cell sensitivity to drugs and growth curves were measured by MTT assay. Changes of cell morphology and ultrastructure were examined by optical and electron microscopy. Results: Solubility, crystallinity, and the crystal properties of an active pharmaceutical ingredient play a critical role in the value chain of pharmaceutical development, manufacturing, and formulation. The rate of drug release for formulation stored at 45°C ± 1°C was increased as compared with the fresh formulation; it might be due to the formation of more pores in the nanoparticles due to evaporation of residual amount of solvent. The in vitro release studies of drug-loaded nanoparticles were conducted at 37 ± 0.5 and 100 rpm using phosphate buffer pH 7.4 (900 ml) in a USP dissolution apparatus under sink condition. DOC-loaded gelatin nanoparticles depleted the viability of MCF human breast cell line. In this study, MCF BC cell line revealed growth inhibition in a dose-dependent manner when treated DOC-loaded gelatin nanoparticles at concentrations ranging from 5 to 100 µg. Conclusion: The DOC-loaded gelatin nanoparticles displayed differential cytotoxicity toward MCF7 cancer cells. These biogenic nanoparticles are biocompatible and found to be good candidates for sustained drug delivery in diseases like cancer.

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In-vitrocytotoxic screening ofGlycyrrhiza glabraL. (fabaceae): A natural anticancer drug

Cited by 23 publications

References 8 publications

Toxic potentials of ten herbs commonly used for aphrodisiac effect in Turkey

Toxic potentials of ten herbs commonly used for aphrodisiac effect in Turkey

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

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