In vitro comparison of the effectiveness of polihexanide and chlorhexidine against canine isolates of Staphylococcus pseudintermedius, Pseudomonas aeruginosa and Malassezia pachydermatis

Banović, Frane; Božić, Frane; Lemo, Nikša

doi:10.1111/vde.12048

Cited by 18 publications

(19 citation statements)

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“…Household bleach (Clorox bleach; 6.15% of sodium hypochlorite; The Clorox Company, Oakland, CA, USA) from unopened containers was diluted with tap water to a final concentration of 0.05%, which was used as the starting concentration. The in vitro antimicrobial activity of diluted sodium hypochlorite against the bacteria S. pseudintermedius ( n = 10) and P. aeruginosa ( n = 10) and the yeast M. pachydermatis ( n = 10), after 3 and 5 min contact time, was evaluated by broth microdilution following a previously described protocol . Break‐point dilution or the minimal bactericidal concentration (MBC) and minimal fungicidal concentration (MFC) was defined as the highest dilution or lowest concentration required for killing the strain.…”

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confidence: 55%

“…The in vitro antimicrobial activity of diluted sodium hypochlorite against the bacteria S. pseudintermedius (n = 10) and P. aeruginosa (n = 10) and the yeast M. pachydermatis (n = 10), after 3 and 5 min contact time, was evaluated by broth microdilution following a previously described protocol. 3 Break-point dilution or the minimal bactericidal concentration (MBC) and minimal fungicidal concentration (MFC) was defined as the highest dilution or lowest concentration required for killing the strain. The difference in MBC and MFC distribution among different groups (type of microbial organism, exposure time) was assessed by post-ANOVA Newman-Keuls test.…”

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confidence: 99%

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In vitro evaluation of the use of diluted sodium hypochlorite (bleach) against Staphylococcus pseudintermedius,Pseudomonas aeruginosa and Malassezia pachydermatis

Banović

Lemo

2014

Veterinary Dermatology

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confidence: 55%

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confidence: 99%

In vitro evaluation of the use of diluted sodium hypochlorite (bleach) against Staphylococcus pseudintermedius,Pseudomonas aeruginosa and Malassezia pachydermatis

Banović

Lemo

2014

Veterinary Dermatology

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“…39-45 PHMB has been used clinically for the treatment of amoebic keratitis.46 Against filamentous fungi, PHMB has previously only been tested in strains recovered from ocular infections and domestic pets. 47-53 In addition to its use in cosmetics and ocular products, several FDA-approved wound cleansing products are currently marketed featuring PHMB as the primary active ingredient. Given the severe morbidity associated with soft-tissue IFI, the consequences and constraints of currently available conventional therapies, and availability of several FDA approved compounds for topical use, we examined the antifungal activity PHMB and MH against a panel of molds isolated from clinical soft-tissue invasive fungal infections occurring in our facility.…”

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confidence: 99%

In Vitroactivity of Manuka Honey and polyhexamethylene biguanide on filamentous fungi and toxicity to human cell lines

et al. 2016

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Soft-tissue invasive fungal infections are increasingly recognized as significant entities directly contributing to morbidity and mortality. They complicate clinical care, requiring aggressive surgical debridement and systemic antifungal therapy. To evaluate new topical approaches to therapy, we examined the antifungal activity and cytotoxicity of Manuka Honey (MH) and polyhexamethylene biguanide (PHMB). The activities of multiple concentrations of MH (40%, 60%, 80%) and PHMB (0.01%, 0.04%, 0.1%) against 13 clinical mold isolates were evaluated using a time-kill assay between 5 min and 24 h. Concentrations were selected to represent current clinical use. Cell viability was examined in parallel for human epidermal keratinocytes, dermal fibroblasts and osteoblasts, allowing determination of the 50% viability (LD50) concentration. Antifungal activity of both agents correlated more closely with exposure time than concentration. Exophiala and Fusarium growth was completely suppressed at 5 min for all PHMB concentrations, and at 12 and 6 h, respectively, for all MH concentrations. Only Lichtheimia had persistent growth to both agents at 24 h. Viability assays displayed concentration-and time-dependent toxicity for PHMB. For MH, exposure time predicted cytotoxicity only when all cell types were analyzed in aggregate. This study demonstrates that MH and PHMB possess primarily time-dependent antifungal activity, but also exert in vitro toxicity on human cells which may limit clinical use. Further research is needed to determine ideal treatment strategies to optimize antifungal activity against molds while limiting cytotoxicity against host tissues in vivo.

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“…50 Its antimicrobial activity does not appear to be impaired by the presence of blood or albumin and the optimal pH range is between 5 and 6. 52,55 No literature is available on the residual action of PHMB. A recent review article by Fjeld reported that the number of clinical studies on PHMB are small and not necessarily well controlled.…”

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Comparative efficacy of three antiseptics as surgical skin preparations in dogs

Boucher

Henton²,

Becker

et al. 2018

Veterinary Surgery

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Objective To compare the antimicrobial efficacy of a 2% chlorhexidine gluconate and 70% ethanol solution (CG+A) with that of F10 Skin Prep Solution (F10) and electrochemically activated water (EAW) when used as a surgical preparation in canine patients. Study design Prospective randomized clinical study. Sample population One hundred sixteen dogs presented for ovariohysterectomy. Methods Dogs were randomly divided into 1 of the 3 antiseptic groups (CG+A, F10, EAW). Skin samples with replicating organism detection and counting plates were taken at 4 different perioperative sites and time intervals (postskin preparation, postskin antisepsis, 2 hours after the second sample, and at the end of surgery) during ovariohysterectomies performed by students. The colony forming unit (CFU) counts from each sample were quantified according to the level of bacterial contamination. Zero CFU was defined as no contamination, 1‐12 CFU was defined as low contamination, and greater than 12 CFU was defined as high contamination. The 3 antiseptics were compared with respect to the level of contamination. Results There was no difference in the level of colonization between the antiseptics at the first sampling time (P = .454). However, the level of contamination for CG+A was lower compared with F10 and EAW at the second, third, and fourth sampling times (P = .001, P = .01, P = .02, respectively). Conclusion CG+A was more effective at achieving a zero CFU count and low levels of contamination compared with F10 and EAW for surgical preparation in dogs undergoing ovariohysterectomy. Clinical significance This study does not provide evidence to support the use of F10 and EAW instead of CG+A for the surgical skin preparation of dogs undergoing ovariohysterectomy.

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In vitro comparison of the effectiveness of polihexanide and chlorhexidine against canine isolates of Staphylococcus pseudintermedius, Pseudomonas aeruginosa and Malassezia pachydermatis

Abstract: The results indicate that polihexanide has comparable in vitro antimicrobial efficacy to chlorhexidine and presents a potential alternative agent for skin and wound antisepsis in veterinary medicine.

Cited by 18 publications

References 33 publications

In vitro evaluation of the use of diluted sodium hypochlorite (bleach) against Staphylococcus pseudintermedius,Pseudomonas aeruginosa and Malassezia pachydermatis

In vitro evaluation of the use of diluted sodium hypochlorite (bleach) against Staphylococcus pseudintermedius,Pseudomonas aeruginosa and Malassezia pachydermatis

In Vitroactivity of Manuka Honey and polyhexamethylene biguanide on filamentous fungi and toxicity to human cell lines

Comparative efficacy of three antiseptics as surgical skin preparations in dogs

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