In vitro cardiomyogenic differentiation of adipose‐derived stromal cells using transforming growth factor‐β1

Abstract

Transplanting stem cells differentiated towards a cardiac lineage can regenerate cardiac muscle tissues to treat myocardial infarction. In this study, we tested the hypothesis that transforming growth factor-b1 (TGF-b1) induces cardiomyogenic differentiation of adiposederived stromal cells (ADSCs) in vitro. Rat ADSCs were cultured with TGF-b1 (10 ng ml À1 ) for 2 weeks in vitro. ADSCs cultured without TGF-b1 served as a control. The mRNA expression of cardiac-specific gene was induced by TGF-b1, while the control culture did not show cardiac-specific gene expression. Immunocytochemical analyses showed that a small fraction of ADSCs cultured with TGF-b1 for 2 weeks stained positively for cardiac myosin heavy chain (MHC) and a-sarcomeric actin. Flow cytometric analyses showed that the proportion of cells expressing cardiac MHC increased with TGF-b1. However, no mesenchymal differentiation (e.g., osteogenic and adipogenic differentiation) was detected other than cardiomyogenic differentiation. These results showed that TGF-b1 induce ADSC cardiomyogenic differentiation in vitro, which could be useful for myocardial infarction stem cell therapy.

Mesenchymal stem cells or cardiac progenitors for cardiac repair? A comparative study

“…To determine which cell type has the best capability to differentiate into cardiomyocytes, the cardiomyogenic differentiation potential of all three cell types was tested by setting up monocultures in medium with low serum concentration (0 or 2%). Since the cardiomyogenicinducing characteristics of TGF-b have been described in some reports [17,27], different concentrations of TGF-b were used as additional cardiomyogenic stimulus. Differentiation was assessed by analyzing the expression of cardiac-specific genes by RT-PCR (Fig.…”

A brief review: adipose-derived stem cells and their therapeutic potential in cardiovascular diseases

“…Although Ang II had a lower inductive efficiency compared to 5-Aza (18% vs. 21%), it was nontoxic to the cells and could be a good substitution for 5-Aza. Moreover, Gwak et al used TGF-β1 to induce ADSCs to differentiate into CMs, as detected by immunofluorescence and flow cytometry [19]. …”

Adipose-Derived Stems Cells and Their Role in Human Cancer Development, Growth, Progression, and Metastasis: A Systematic Review

“…They have the ability to differentiate into numerous cell lineages, including adipogenic, osteogenic, chondrogenic, myogenic, cardiomyogenic, and neurogenic-like cell types (9,(21)(22)(23)(24)(25). They have been associated with expressing/secreting multiple important growth factors, cytokines, chemokines, and inflammatory biomarkers linked to cancer development and progression, including insulin-like growth factor (IGF), hepatocyte growth factor (HGF), TGFb1, VEGF, IL8, Bcl-2, and IL10 (26)(27)(28)(29)(30)(31)(32)(33)(34).…”