A brief review: adipose-derived stem cells and their therapeutic potential in cardiovascular diseases

Ma, Teng; Sun, Jiacheng; Zhao, Zhen-Ao; Lei, Wei; Chen, Yueqiu; Wang, Xu; Li, Yangxin; Shen, Zhenya

doi:10.1186/s13287-017-0585-3

Cited by 105 publications

(91 citation statements)

References 57 publications

(69 reference statements)

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“…Human adipose tissue derived stromal cells (ASC) are currently being tested as cell-based therapies against a wide range of diseases and conditions in numerous clinical trials (Bateman et al, 2018) including wound healing, (Bertozzi et al, 2017) cardiovascular disease (Ma et al, 2017) and cartilage regeneration (Pak et al, 2018). ASC are isolated from adipose tissue and are therefore an abundant source of stromal cells that can be accessed with relative ease using the minimally invasive procedure of liposuction.…”

Section: Introductionmentioning

confidence: 99%

Ex Vivo Human Adipose Tissue Derived Mesenchymal Stromal Cells (ASC) Are a Heterogeneous Population That Demonstrate Rapid Culture-Induced Changes

et al. 2020

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Human adipose-derived mesenchymal stromal cells (ASC) are showing clinical promise for the treatment of a range of inflammatory and degenerative conditions. These lipoaspirate-derived cells are part of the abundant and accessible source of heterogeneous stromal vascular fraction (SVF). They are typically isolated and expanded from the SVF via adherent cell culture for at least 2 weeks and as such represent a relatively undefined population of cells. We isolated ex vivo ASC directly from lipoaspirate using a cocktail of antibodies combined with immunomagnetic bead sorting. This method allowed for the rapid enrichment of a defined and untouched ex vivo ASC population (referred to as MACS-derived ASC) that were then compared to culturederived ASC. This comparison found that MACS-derived ASC contain a greater proportion of cells with activity in in vitro differentiation assays. There were also significant differences in the secretion levels of some key paracrine molecules. Moreover, when the MACS-derived ASC were subjected to adherent tissue culture, rapid changes in gene expression were observed. This indicates that culturing cells may alter the clinical utility of these cells. Although MACS-derived ASC are more defined compared to culture-derived ASC, further investigations using a comprehensive multicolor flow cytometry panel revealed that this cell population is more heterogeneous than previously appreciated. Additional studies are therefore required to more precisely delineate phenotypically distinct ASC subsets with the most therapeutic potential. This research highlights the disparity between ex vivo MACS-derived and culture-derived ASC and the need for further characterization.

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Section: Introductionmentioning

confidence: 99%

Ex Vivo Human Adipose Tissue Derived Mesenchymal Stromal Cells (ASC) Are a Heterogeneous Population That Demonstrate Rapid Culture-Induced Changes

et al. 2020

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“…With Adipose-derived stem cells (ADSCs) are pluripotent stem cells developed from the mesoderm and possess multipotential differentiation potential [8]. It can differentiate into chondrocytes [9], adipocytes, cardiomyocytes [10], odontoblasts [11,12], and nerve cells [13] under different induction culture conditions. It has many advantages such as easy access, fast proliferation, low immunogenicity, and stable biological performance [14].…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Wnt Pathway on Cartilage Differentiation of Adipose Derived Stem

Dai

Zhang

Huang

et al. 2020

Preprint

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Objective Exploring the interaction of Wnt signaling pathway and sox9 to elucidate the role of adipose-derived stem cells in chondrogenic differentiation. Methohs Through LiCl,DKK-1 stimulate ADSCs into cartilage differentiation, detection of middle-late late early differentiation into cartilage indicators such as collagen type II (Collagen2a), gather the proteoglycan (Aggrecan) and Wnt pathways key protein Sox9, BMP-2, beta serial protein (beta-catenin), glycogen synthase kinase 3 beta (GSK-3 beta) expression.Subsequently, lentivirus was used as the vector, the full-length Sox9 gene was transfected into ADSCs for stable expression, and the expression levels of relevant indicators were detected again. Results During the chondrogenic induction and differentiation of ADSCs, the Wnt pathway promoted the rapid proliferation of ADSCs in the early, middle and late stages and induced the expression of Collagen 2a Aggrecan to increase in the early stage but not significantly (P<0.05).At the later stage, the phenotype of mature cartilage was inhibited and cartilage hypertrophy and early cartilage ossification were promoted.Overexpression was fed back to inhibit the expression of Sox9, the activity of Wnt pathway, up-regulated in early, mid-stage and down-regulated in late stage (P<0.01). Conclusion The Wnt pathway regulates chondrogenic differentiation of ADSCs by regulating Sox9, but its regulatory mechanism is significantly different at different stages of induced differentiation.

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“…Adipose-derived stem cells (ADSCs) are pluripotent stem cells developed from the mesoderm and possess multipotential differentiation potential [8]. It can differentiate into chondrocytes [9], adipocytes, cardiomyocytes [10], odontoblasts [11,12], and nerve cells [13] under different induction culture conditions.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Wnt Pathway on Cartilage Differentiation of Adipose-Derived Stem cells

Dai¹,

Zhang²,

Huang³

et al. 2020

Preprint

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Background: Adipose-derived stem cells ( ADSCs ) therapy is considered as a promising alternative to treat osteoarthritis (OA).Considerable evidence has shown that the Wnt signaling pathway is involved in chondrogenic differentiation in various stem cells.In the present study, we explored in detail the mechanism of of Wnt signaling pathway in the regulation of adipose-derived stem cell chondrogenic differentiation.Methohs: Adipose-derived stem cells were extracted from Sprague-Dawley groin area, isolated, cultured and verified. Then LiCl and DKK-1 used to induce ADSCs cartilage differentiation to detect indicators of cartilage at different stages, such as collagen type II (Collagen2a), aggrecan (Aggrecan) and Wnt pathway key proteins Sox9, β-catenin, Glycogen synthase kinase-3β (GSK-3β) expression.Subsequently, lentivirus was used as the vector, the full-length Sox9 gene was transfected into ADSCs and the expression levels of relevant indicators were detected again after stable expression.Results: In the process of inducing differentiation, the Wnt pathway promoted the rapid proliferation of ADSCs in the early, middle and late stages, and up-regulates Sox9 expression.Cartilage indicators Collagen 2a and Aggrecan expression levels were not significantly increased in the early stage, but significantly increased in the middle and late stages (P <0.05). At the later stage, however, the Wnt pathway down-regulated Sox9 expression, weakened the mature cartilage phenotype, promoted cartilage hypertrophy and early cartilage ossification. Finally, the overexpression of Sox9 feedback inhibits the Wnt pathway activity, down-regulates β-catenin expression, maintains cartilage phenotype, delays cartilage hypertrophy and early osteogenesis.Conclusion:The Wnt pathway regulates chondrogenic differentiation of ADSCs by regulating Sox9, but its regulatory mechanism is significantly different at different stages of induced differentiation.

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A brief review: adipose-derived stem cells and their therapeutic potential in cardiovascular diseases

Cited by 105 publications

References 57 publications

Ex Vivo Human Adipose Tissue Derived Mesenchymal Stromal Cells (ASC) Are a Heterogeneous Population That Demonstrate Rapid Culture-Induced Changes

Ex Vivo Human Adipose Tissue Derived Mesenchymal Stromal Cells (ASC) Are a Heterogeneous Population That Demonstrate Rapid Culture-Induced Changes

Mechanism of Wnt Pathway on Cartilage Differentiation of Adipose Derived Stem

Mechanism of Wnt Pathway on Cartilage Differentiation of Adipose-Derived Stem cells

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