In VitroBiomimetic Model of the Human Immune System for Predictive Vaccine Assessments

In vitro innate immune cell based models to assess whole cell Bordetella pertussis vaccine quality: A proof of principle

“…Functional immunological assays could complement this approach and ultimately reduce animal based lot release testing. Although previous studies have shown that APC platforms can be used in vitro to determine vaccineinduced responses in R&D [26,27], this is the first evaluation of these types of APC platforms for monitoring vaccine quality. Here, we provide proof-of-principle that moDC and MM6 cells can be used to monitor the quality of wP vaccine in vitro, although validation experiments will be necessary.…”

Neuraminidase-Inhibiting Antibody Is a Correlate of Cross-Protection against Lethal H5N1 Influenza Virus in Ferrets Immunized with Seasonal Influenza Vaccine

“…The antibody forensics (AF) method was used for measuring the levels of strain-specific IgG to HA in ferret sera (22). This is a multiplexed bead array technique based on the BioPlex/Luminex platform, in which serum is incubated with arrays of fluorescent beads coated with recombinant hemagglutinins derived from different influenza strains.…”

The in vitro MIMIC® platform reflects age-associated changes in immunological responses after influenza vaccination

“…To investigate the ability of the MIMIC Ò system to reflect the influence of age on the generation of functional anti-TIV antibodies, we determined the capacity of adult vs. elderly donors to inhibit hemagglutination using a novel, highly-sensitive HAI assay developed in our laboratory, the surface-assisted hemagglutination inhibition (SA-HAI) assay. Previous work from our group has demonstrated a strong correlation between HAI and SA-HAI ( [5] and manuscript in preparation). Fig.…”

“…SA-HAI is a patented technology that involves modifications to traditional HAI previously described elsewhere (Patent # 8,962,256 B2 and [5]). In this study, hemagglutination titers for BPL-inactivated A/California/07/2009 H1N1 and B/Massachusetts/2/2012 viruses (Influenza Reagent Resource) and formalin-inactivated vaccine grade A/Texas/50/2012 H3N2 (Sanofi Pasteur) were determined by incubation of duplicate 2-fold serial dilutions, starting at 1:100, in an immunograde surface 96-well BRANDplate (BrandTech Scientific Inc) for 15 h at 4°C while shaking followed by a 2 h PBS wash.…”

“…Recent technological advances in 2D and 3D culturing of human cells have led to major breakthroughs in the field of in vitro disease modeling. These systems range from basic immunogenicity testing via ex vivo culture of PBMCs to 3D human artificial lymph node (HuALN) bioreactors (reviewed in [4] such diverse targets as HBV, tetanus toxoid, monoclonal antibodies and YF-VAX [5][6][7]. In this study, we have extended those findings to include the differential immune responses of adult versus elderly donors to vaccination against seasonal influenza.…”