<i>In‐vitro</i> assays of polymer‐coated stents eluting platelet glycoprotein IIb/IIIa receptor monoclonal antibody

Yin, Tieying; Wang, Guixue; Ruan, Changgeng; Guzman, Randolph; Guidoin, Robert

doi:10.1002/jbm.a.31369

Cited by 15 publications

(12 citation statements)

References 50 publications

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“…SZ‐21 (Jiangshu Institute of Hematology, Suzhou, China), the mAb against platelet GP IIIa, can inhibit platelet aggregation after the antibody is absorbed by the PLLA polymer coating on the surface of the stents 13. As shown in the present experimental results, the SZ‐21‐eluting stent can inhibit platelet adherence and aggregation, thus preventing stent thrombosis in vivo .…”

Section: Discussionsupporting

confidence: 60%

“…The mAb‐eluting stents were prepared by passive absorption method. The presence of the PLLA polymer coating and the mAb on the entire surface of the stent was examined using fluorescence staining and immunofluorescence technique, respectively 13. The adsorption characteristics of the stents exposed to the mAb were tested by radioisotope technique with 125 I‐labelled SZ‐21.…”

Section: Methodsmentioning

confidence: 99%

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Amphiphilic diblock copolymers containing poly(N‐hexylisocyanate): Monolayer behavior at the air–water interface

Gargallo

Becerra

Sandoval

et al. 2011

J of Applied Polymer Sci

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The behavior of amphiphilic diblock copolymers containing 80-89% of poly(N-hexylisocyanate) (PHIC) with different hydrophobic segments spread at the air-water interface has been studied. Surface pressure-area isotherms (p-A) at the air-water interface were determined. It was found that these diblock copolymers form stable monolayers and the isotherms present a pseudoplateau region at low surface pressure, irrespective of the nature of the partner block: poly(styrene) (PS) or poly(isoprene). Surface pressure variation at the semidilute region of the monolayer was expressed in terms of the scaling laws as power function of the surface concentration. The critical exponents of the excluded volume m obtained for copolymers with PHIC and PS blocks are 0.58 for the copolymer with 85% of PHIC and 15% of PS, and 0.63 for the copolymer with 89% of PHIC and 11% of PS. The hydrophobicity degree of the diblock copolymers was estimated from the determination of the surface energy values by wettability measurements. The morphology of the monolayers was determined by means of Brewster angle microscopy. Molecular dynamic simulation was performed to explain the experimental behavior of diblock copolymers at the air-water interface.

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Section: Discussionsupporting

confidence: 60%

Section: Methodsmentioning

confidence: 99%

Amphiphilic diblock copolymers containing poly(N‐hexylisocyanate): Monolayer behavior at the air–water interface

Gargallo

Becerra

Sandoval

et al. 2011

J of Applied Polymer Sci

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“…The SZ‐21 has the ability to block the GP IIb/IIIa receptors on the platelets, preventing the formation of fibrinogen bridges, the final common pathway in platelet thrombus formation 30. Although the chitosan is often used as a procoagulant material, the hemostatic role of chitosan is indirect, through its nonspecific adsorption of blood cells, rather than a direct activation of the blood coagulation system 46.…”

Section: Discussionmentioning

confidence: 99%

Layer‐by‐layer assembly of chitosan and platelet monoclonal antibody to improve biocompatibility and release character of PLLA coated stent

Luo

Wang

et al. 2011

J Biomedical Materials Res

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The aim of this study is to construct a biocompatible coating of a drug-eluting stent through the incorporation of chitosan with monoclonal antibody (mAb) to a platelet glycoprotein (GP) IIIa receptor, by electrostatic layer-by-layer (LBL) adsorption of oppositely charged polyelectrolytes and proteins. The platelet maximum aggregation rate and aggregation inhibition rate tests confirm the bioactivity of mAb in different pH assembly environments. The fluorescence spectra test and confocal laser scanning microscopy observation were used to monitor the LBL assembly process of the mAb/chitosan multilayer on the surface of the aminolyzed Poly-L-lactic acid (PLLA) membrane, when using Rhodamine B isothiocyanate-labeled mAb and Fluorescein isothiocyanate-labeled chitosan. The in vitro platelet adhesion experiment demonstrated the amicable blood compatibility of the mAb/chitosan multilayer. The endothelial cell adhesion and migration test revealed that the multilayer could improve the cytocompatibility of the PLLA matrix in terms of cell attachment, proliferation, and migration. An in vitro perfusion circuit was designed to evaluate the release rates measured by a radioisotope technique with ¹²⁵I-labeled GP IIIa mAb. The different eluting curves of the mAb/chitosan-assembled stent and mAb physically absorbed stent showed the improvement of mAb's release character when using LBL self-assembly technology. Our method to prepare a biocompatible stent surface with mAb/chitosan multilayers has proved to be favorable and effective in vitro, thus justifying further evaluation to improve the biocompatibility in an animal model test.

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“…The Nitinol wires were coated with PLLA using previously described methods [23] and were processed according to different protocols for the adsorption of the mAb SZ-21. Protocol 3.…”

Section: Methodsmentioning

confidence: 99%

Eluting characteristics of a platelet glycoprotein receptor antibody using a PLLA-coated stent

Wang

Yin²,

Luo

et al. 2008

Journal of Biomaterials Science, Polymer Edition

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Drug-eluting stents (DES) have emerged as a recognized alternative to treat stent restenosis but many questions remain regarding the optimal type and eluting characteristics of both drug and stent. The first component of the study examines the extent of surface coating of PLLA (poly(L-lactic acid)) on a Nitinol stent. The second characterizes the adsorption and elution rates of monoclonal mouse anti-human platelet glycoprotein (GP) IIIa antibody SZ-21 from a PLLA-coated surface. The PLLA coating was examined by fluorescence staining and image analysis using the Image Processing Box of MATLAB. Stents exposed to the monoclonal mouse anti-human platelet GP IIIa antibody were tested for their adsorption characteristics by radioisotope technique with (125)I-labelled SZ-21. The elution rates were then measured in looped circuits at different velocities (10 or 20 ml/min) and durations (30 min up to 312 h). Results showed that the fluorescence staining and image analysis showed a striking difference in the extent of coating between PLLA-coated stents and SZ-21 eluting stents on the gray-scale distribution of Nitinol surfaces. The amount of SZ-21 adsorbed onto the PLLA-coated stents was dependent on the concentration and duration of immersion in the solution. The method of preparation the mAb eluting stent significantly influenced the elution characteristics for a continuous perfusion of more than 300 h. The eluting curve was biphasic with initial rapid elution for the first 24 h followed by a gradual slow elution. These results indicate that the Image Processing Box of MATLAB appears to be a useful method for semi-quantitative analysis of fluorescence images. Furthermore, SZ-21 can be passively adsorbed onto PLLA-coated stents and predictably influenced by the concentration and duration of immersion. These studies may pave the way to developing stent-based delivery of a potent anti-platelet agent.

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In‐vitro assays of polymer‐coated stents eluting platelet glycoprotein IIb/IIIa receptor monoclonal antibody

Cited by 15 publications

References 50 publications

Amphiphilic diblock copolymers containing poly(N‐hexylisocyanate): Monolayer behavior at the air–water interface

Amphiphilic diblock copolymers containing poly(N‐hexylisocyanate): Monolayer behavior at the air–water interface

Layer‐by‐layer assembly of chitosan and platelet monoclonal antibody to improve biocompatibility and release character of PLLA coated stent

Eluting characteristics of a platelet glycoprotein receptor antibody using a PLLA-coated stent

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