In vitro antioxidant effect of curcumin on human sperm quality in leucocytospermia

Abstract

Decreased sperm quality was caused by oxidative stress in semen from patients with leucocytospermia. Curcumin is a traditional Chinese herbal monomer extracted from Zingiberaceae turmeric and zedoary turmeric and has antioxidative and anti-inflammatory effects. This study aimed to investigate the effects and specific molecular mechanisms of curcumin on sperm quality in patients diagnosed with leucocytospermia. Forty cases of semen samples were collected from patients with leucocytospermia and 35 cases from normal fertile male. Computer-assisted semen analysis (CASA) was used to detect sperm motility after curcumin incubation. ELISA was used to measure the changes in H O , sperm mitochondrial DNA (mtDNA), cytochrome B (Cyt B) and NADH dehydrogenase 5 (NADH5) contents before and after curcumin treatment. The results indicate that curcumin can significantly improve sperm motility from the patients with leucocytospermia. After curcumin treatment, the level of the H O was significantly decreased in the supernatant of curcumin-incubated spermatozoa from leucocytospermic patients. The content of mtDNA was significantly decreased, while the content of Cyt B and NADH5 in spermatozoa was significantly increased. In conclusion, curcumin can significantly improve sperm motility of leucocytospermic patients, against the oxidative damage induced by H O . Therefore, curcumin may play a role in mitigating the H O -induced injury to sperm.

Diarylheptanoids as nutraceutical: A review

“…It affects the mobility of human spermatozoa and its function in vitro and in vivo fertility (Naz and Lough, 2014). Studies have demonstrated the potential of curcumin for the development of a novel intravaginal contraceptive (Zhang et al, 2017). Diarylheptanoids isolated from D. spongiosa such as diospongin B and C are found to exhibit anti-osteoporotic activity by inhibiting the release of 45 Ca on the resorption of bone tissues, the same was compared with standard drug elcitonin (Yin et al, 2004a).…”

Environmental Impact on Male (In)Fertility via Epigenetic Route

“…Polyphenols, such as resveratrol and curcumin, are well known agents able to modulate methyltransferase and histone deacetylase enzyme activities, thus being potential candidates for intentional modulation of epigenetic dynamics in spermatogenesis [ 248 ]. Indeed, such molecules are under investigation in the field, showing promising beneficial effects in ameliorating spermatogenesis in obese conditions both in animal models and in humans [ 275 , 276 , 277 , 278 ]. Concentration of factors as zinc in seminal fluid modulates protamine formation and dynamically chromatin structure, as a consequence [ 279 ].…”

Prospects for the Use of Indole-3-carbinol in the Treatment of Benign Prostatic Hyperplasia

“…В исследованиях іn vitro было установлено антиоксидантное действие куркумина на качество спермы человека при лейкоцитоспермии. Проведение инкубации спермы с признаками лейкоспермии с куркумином с последующим компьютерным анализом спермы установило снижение в ней уровня гидроперекисей и повышение подвижности сперматозоидов [48].…”