In vitroantioxidant and protective effects of corn peptides on ethanol-induced damage in HepG2 cells

Abstract

To assess the effect of corn peptides (CPs) as an antioxidant on ethanol (EtOH)induced oxidative stress in HepG2 cells, the cells were preincubated with CPs for 4 h before treated with EtOH. Methyl thiazolyl tetrazolium and flow cytometry assay were performed to detect the viability of CP-treated HepG2 cells and control cells. The activities of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined by corresponding kits, respectively. The results showed that treatment with 300 mmol/L EtOH for 24 h resulted in a death of around 50% of the HepG2 cells. Pretreatments of appropriate concentrations of CPs (more than 5 mg/mL, particularly at 20 mg/ mL) significantly prevented EtOH-induced cytotoxicity and reduced the increase of ALT, AST, LDH and MDA contents, as well as inhibited the reduction of SOD contents in the HepG2 cells, indicating that CPs protected the cells from oxidative damage induced by EtOH metabolism, and thus had a great application potential in hepatoprotective nutraceuticals.

A Comprehensive Review of Corn Protein‐derived Bioactive Peptides: Production, Characterization, Bioactivities, and Transport Pathways

“…For example, CPs with a MW less than 3 kDa not only exhibited the antihypertensive activity, but also had excellent antioxidant activity (Huang et al, 2011). Multiple bioactivities of identical CPs, including antioxidant and hepatoprotective as well as alcohol-metabolismfacilitating and hepatoprotective, have been corroborated in previous studies (She et al, 2016;Yu et al, 2012).…”

Hepatoprotective Effect of Albumin Peptides from Corn Germ Meal on Chronic Alcohol‐Induced Liver Injury in Mice

“…Bioactive substances with antioxidants activities that could prevent alcohol‐induced liver injury were found in both plants and animals, such as lucidone from the fruits of Lindera lucida (Senthil Kumar and others ), polysaccharides from the peduncles of Hovenia dulcis (Wang and others ), peptide S‐8300 from shark (Huang and others ), and corn peptides from corn gluten meal (She and others ; Ma and others ; Wu and others ; Zhang and others ). Corn gluten meal, a by‐product from the manufacture of corn starch, contains approximately 60% (W/W) protein (Zhuang and others ).…”

Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells

“…The comet assay is a rapid, sensitive, and relatively simple method for analyzing DNA damage at the level of individual cell (She et al, 2016). The amount of DNA that migrates away from the nuclei is used to assess the extent of DNA damage.…”

“…Various environmental stresses are known to enhance the ROS production, such as ultraviolet radiation, heavy metals and pesticide remnant, disturbing the cellular redox homeostasis (Ma et al, 2014;Shahid et al, 2014;Thévenod, Friedmann, Katsen, & Hauser, 2000). Increase of ROS elicits a wide variety of physiological and cellular events, including lipid peroxidation, antioxidants' depletion, and DNA damage (Chen et al, 2012;She et al, 2016;Wang, Martínez, et al, 2016). The cellular antioxidant enzymes mainly include superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferases (GSTs), which play a crucial role in scavenging ROS, but overexposure causes their depletion, leading to disruption of core metabolic and regulatory process (Marin & Taranu, 2012;Mejdoub, Fahde, Loutfi, & Kabine, 2017).…”