<i>In vitro</i>antioxidant activity of<i>Retama monosperma</i>(L.) Boiss.

Belmokhtar, Zoubir; Harche, M.

doi:10.1080/14786419.2014.934237

Cited by 12 publications

(8 citation statements)

References 16 publications

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“…As the reduced form of DPPH is pale yellow, the greater the free radical scavenging capacity of an antioxidant compounds, is identified by the more reduction of DPPH and the less purple color there is in the sample 37 . Our result clearly indicated that the ethyl acetate fraction presented an inhibition percentage of quite higher than other fractions (92,436 %) which was in agreement with the results reported in the literature 7,22,38,39 , indicating better DPPH free radical scavenging activity in the ethyl acetate solvent. This good antioxidant activity might be attributed to the presence of high amounts of phenolic compounds in this fraction.…”

Section: Total Antioxidant Capacitysupporting

confidence: 92%

Assessment of Antioxidant Potential and phytochemical screening of Phenolic Compounds of Gleditsia triacanthos L Pods

Saidi¹,

Hassiba

Abbassia³

2018

ijmsci

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Objective: The present study aims at the phytochemical screening, the quantification of phenolic compounds of Gleditsia triacanthos L pods (Family Leguminosae) and the assessment of their antioxidant potential by in vitro assays. Subjects and Methods: The pods were extracted with 70% methanol and further partitioned with petroleum ether, chloroform, ethyl acetate, and n-butanol. The residual aqueous fraction has been also recovered. Colorimetric methods using Folin-Ciocalteu reagent, aluminum chloride and Folin-Denis were carried out to estimate total polyphenols, flavonoids and condensed tannins content of extracts. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant capacity (TAC) were used to determine in vitro antioxidant activity. Results: In vitro phytochemical screening for all the extracts was tested and shown positive result for flavonoids, tannins, cardiac glycosides, sterols and terpenes, saponins and alkaloids. However, all the extracts were free of anthraquinones. The strongest activity against radical scavenging of DPPH was found in the ethyl acetate fraction (IC 50 = 16,288 ± 0,299 µg/ml) which contains highest amounts of flavonoids (25.160 ± 0.016 mg CE/g), whereas the chloroform fraction showed an important total antioxidant capacity (750,584 ± 129,793 mg AAE/g) with the highest amount of total polyphenols (131.667 ± 2.055 mg GAE/g). When compared to the other fractions, the aqueous fraction presented the lowest antioxidant activity for the two methods. Conclusion: These data suggest that the pods of Gleditsia triacanthos L can be a good natural source of antioxidants that can be beneficial for food and human health.

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Section: Total Antioxidant Capacitysupporting

confidence: 92%

Assessment of Antioxidant Potential and phytochemical screening of Phenolic Compounds of Gleditsia triacanthos L Pods

Saidi¹,

Hassiba

Abbassia³

2018

ijmsci

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“…Polyphenols, flavonoids, and tannins, which are organic compounds found abundantly in plants, have become an emerging field of interest in nutrition in recent decades. Retama monosperma can be considered one of the plants rich in those compounds, as proven by several studies [ 3 , 13 , 14 , 15 , 16 ]. Previous studies have aimed to identify specific compounds present in R. monosperma through various identification methods [ 16 , 29 ].…”

Section: Chemical Compositionmentioning

confidence: 99%

“…Previous studies have aimed to identify specific compounds present in R. monosperma through various identification methods [ 16 , 29 ]. Conversely, other authors have chosen to qualitatively quantify the content of certain groups of molecules, such as polyphenols, flavonoids, and condensed tannins, without identifying each individual compound [ 13 , 14 ]. Belmokhtar et al, 2014 reported that ethyl acetate extract has the highest polyphenol content in any of the stems, flowers, and seeds [ 13 ], while Hamdani et al recorded in 2018 that the highest total polyphenols contents were reported in the methanol extract of the stems and the methanolic extract of the flowers showed a higher tannins content than the other extracts [ 14 ].…”

Section: Chemical Compositionmentioning

confidence: 99%

“…R. monosperma is considered one of the medicinal plants rich in bioactive compounds, including alkaloids, polyphenols, flavonoids, fatty acids, and condensed tannins, as proved by several studies [ 3 , 13 , 14 , 15 , 16 ]. It has been extensively used in folk medicine in a wide range of countries; for example, used as an effective antihelmintic, disinfectant, and abortifacient and also to treat skin damages and cicatrization [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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A Comprehensive Review of the Pharmacological Properties and Bioactive Components of Retama monosperma

et al. 2023

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Retama monosperma L. (Boiss.) or Genista monosperma L. (Lam.), known locally as “R’tam”, is a spontaneous and annual herb that belongs to the Fabaceae family. It is native to the Mediterranean regions, specifically in the desert areas and across the Middle Atlas in Morocco. This plant has been extensively used in folk medicine and it is rich in bioactive compounds, including polyphenols, flavonoids, and alkaloids. Current research efforts are focusing on the development of novel natural drugs as alternatives to various organic and non-organic chemical products from Retama monosperma. In addition, extract, and isolated compounds obtained from different parts of the chosen plant have been described to exhibit multiple biological and pharmacological properties such as antioxidant, anti-aging, anti-inflammatory, antihypertensive, anti-helminthic, disinfectant, diuretic, and hypoglycemic effects. The plant-derived extract also acts as an antimicrobial agent, which is highly efficient in the treatment of bacterial, viral, and fungal infections. Its antiproliferative effects are associated with some mechanisms, such as the inhibition of cell cycle arrest and apoptosis. In light of these assessments, we critically highlight the beneficial effects of the flowers, stems, seeds extracts, and isolated compounds from R. monosperma (L.) Boiss in human health care, industrial, and other applications, as well as the possible ways to be employed as a potential natural source for future drug discovery.

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“…The relationship between the antioxidant activity and the phenolic contents, flavonoids and condensed tannin of Retama monosperma was evaluated by Belmokhtar et al (2014). It was found that the antioxidant activities of the different fractions (chloroform, ethyl acetate, butanol and methanol) of the hydromethanolic extract of the different parts of this plant using DPPH method represented a significant high correlation between flavonoids content and antioxidant activities (r=0.91).…”

Section: Flavonoids Content Of Leaves and Flowers Ethyl Acetate And Nmentioning

confidence: 99%

Phytochemical analysis, antioxidant and antimicrobial activities of leaves and flowers ethyl acetate and n-butanol fractions from an Algerian endemic plant Calycotome spinosa (L.) Link

Cherfia¹,

Ali²,

Talhi³

et al. 2017

J. Pharmacognosy Phytother.

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Calycotome spinosa (L.) Link is one of the important plants in traditional medicine especially in Algeria. The present work deals with the phytochemical screening, the flavonoids extraction from leaves and flowers of this plant, the quantification of total polyphenols and flavonoids, as well as the evaluation of antioxidant and antimicrobial properties of ethyl acetate and n-butanol fractions. While, the rest, interacted with its phenolics identification. The results of preliminary phytochemical screening showed that C. spinosa contained various metabolites: Polyphenols, flavonoids, alkaloids, saponins etc. In addition, the findings of polyphenols determination using Folin Ciocalteu was high in all the fractions: 107.75±0.41 and 64.24±1.81 mg gallic acid equivalents/g extract for leaves ethyl acetate and n-butanol respectively and 81.45±0.6 and 96.06±2.72 mg gallic acid equivalents/g extract for flowers ethyl acetate and n-butanol successively. Conversely, the flavonoids content in the four fractions by AlCl 3 was less important than polyphenols. Moreover, the obtained fractions were evaluated for their antioxidant capacity using DPPH assay. Besides, the obtained result revealed that Ethyl acetate and n-butanol fractions of leaves exerted very high antioxidant activities with IC 50 equal to 45.25±1.8 and 52.80±2.05 µg/mL, respectively compared with flowers. Furthermore, the antimicrobial activity of those fractions against many microbial strains have also been investigated using the disc diffusion method; the leaves ethyl acetate fraction showed the highest activity against Acinetobacter baumannii with a zone inhibition diameter equal to 22±0.06 mm. Additionally, high-performance liquid chromatography/diodearray detector (HPLC/DAD) analysis performed with C. spinosa fractions revealed the richness of plant in many phenolic compounds.

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In vitroantioxidant activity ofRetama monosperma(L.) Boiss.

Cited by 12 publications

References 16 publications

Assessment of Antioxidant Potential and phytochemical screening of Phenolic Compounds of Gleditsia triacanthos L Pods

Assessment of Antioxidant Potential and phytochemical screening of Phenolic Compounds of Gleditsia triacanthos L Pods

A Comprehensive Review of the Pharmacological Properties and Bioactive Components of Retama monosperma

Phytochemical analysis, antioxidant and antimicrobial activities of leaves and flowers ethyl acetate and n-butanol fractions from an Algerian endemic plant Calycotome spinosa (L.) Link

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