<i>In Vitro</i>Anti-cholinesterase Activity and Mass spectrometric Analysis of<i>Curculigo orchioides</i>Gaertn., Rhizome Extract

Pratap, G.; Rather, Suhail Ahmad; Shantaram, Manjula

doi:10.1080/22297928.2020.1837669

Cited by 5 publications

(8 citation statements)

References 24 publications

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“…Electron impact ionisation was employed (70 eV), and the MS (mass spectrometry) ion source was maintained at 240°C. Compounds were identified by their gas chromatography retention times and mass spectra using standard compounds for comparison with methanolic fraction’s GC-MS graph ( Pratap et al, 2020a ; Pratap et al, 2020b ).…”

Section: Methodsmentioning

confidence: 99%

“…Using quercetin (1 mg/g), a positive control, the total flavonoid content was determined. The equation employed was y = 0.0255x, R2 = 0.9822, where x is the absorbance and y is the positive control of quercetin equivalent (mg/g), based on the calibration curve ( Pratap et al, 2020a ).…”

Section: Methodsmentioning

confidence: 99%

“…According to the bioautographic approach ( Devika & Koilpillai, 2014 ; Pratap et al, 2020a ), the acetylcholinesterase inhibitory activity of the methanolic fractions was discovered using TLC. The methanolic fraction was spotted (10 g/mL) and developed with a standardising mobile-phase (CHCl3:MeOH, 9.5:0.5) before being dried at room temperature on a silica gel-coated TLC plate, 2.5 mm F-254, 10 10 cm (Merck, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…In order to establish the presence of putative AChE inhibition zones, the plates were later dried at room temperature for 1 min, resulting in colourless or white specks on a yellow background. After measuring the Rf value of the plant bioactive components that were calculated for the separation of the molecule in preparative scale, the spots on the plates were confirmed for being possible AChE inhibitors and recognised as clear zones against a yellow background ( Marston et al, 2002 ; Pratap et al, 2020a ; Pratap et al, 2020b ).…”

Section: Methodsmentioning

confidence: 99%

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In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Kenchappa,

Karthik,

Vijendra

et al. 2023

Front. Pharmacol.

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Alzheimer’s disease (AD) is a type of neurodegenerative disease, associated with the hastening of ROS, acetylcholinesterase (AChE) activity, and amyloid β peptides plaques in the brain. The limitations and side effects of existing synthetic drugs incline toward natural sources. In the present communication active principles of methanolic extract of Olea dioica Roxb, leaves are explored as an antioxidant, AChE inhibitor, and anti-amyloidogenic. Furthermore, neuroprotection against the amyloid beta-peptide has been studied. The bioactive principles were identified by GC-MS and LC-MS and further subjected to antioxidant (DPPH and FRAP) and neuroprotection (AChE inhibition, ThT binding, and MTT assay, DCFH-DA and lipid peroxidation (LPO) assay using neuroblastoma (SHSY-5Y) cell lines) assays. Methanolic extract of O. dioica Roxb, leaves was found to contain polyphenols and flavonoids. In vitro assays exhibited potential antioxidant and anti-AChE (˃50%) activities. ThT binding assay indicated protection against amyloid-beta aggregation. MTT assay, Aβ1-40 (10 µM) with extract increase the cell viability (˃50%) and showed significant cytotoxicity to SHSY-5Y cells. ROS level (˃25%) significantly decreased in the Aβ1-40 (10 µM) + extract (15 and 20 μM/mL) and LPO assay (˃50%) suggesting prevention of cell damage. Results advocate that O. dioica leaves are a good source of antioxidants, anti-AChE, and anti-amyloidogenic compounds which may be further evaluated as a natural medicine for the treatment of AD.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Kenchappa,

Karthik,

Vijendra

et al. 2023

Front. Pharmacol.

Self Cite

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“…The experiment showed that the ethanol extract had a good inhibitory effect on the AChE enzyme. This may help to explain the role of methanol extract from rhizome as an anti-acetylcholinesterase drug in the treatment of Alzheimer’s disease [ 73 ]. The curculigoside ( 32 ) in C. orchioides can effectively improve the behavior of Alzheimer’s rats, reduce the damage of hippocampal neurons, and inhibit the apoptosis of hippocampal neurons [ 74 ].…”

Section: Pharmacological Propertiesmentioning

confidence: 99%

Phytochemistry and Pharmacological Activity of Plants of Genus Curculigo: An Updated Review Since 2013

Wang

2021

Molecules

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The genus Curculigo, as a folk herbal medicine, has been used for many years in China, treating impotence, limb limpness, and arthritis of the lumbar and knee joints. The last systematic review of the genus Curculigo was written in 2013, scientifically categorizing the phytochemistry and biological activities. Hitherto, the original compounds and their pharmacological activities were presented as the development of this genus, but there is not an updated review. To conclude the progression of the genus Curculigo, we collected the new literature published from 2013 to 2021 in PubMed, Web of Science, Google Scholar databases, and the Chinese National Knowledge Infrastructure. The novel chlorophenolic glucosides, curculigine, phenolic glycosides, orcinosides and polysaccharides were isolated from Curculigo. The new analyzing methods were established to control the quality of Curculigo as a herbal medicine. In addition, the pharmacological effects of Curculigo focused on anti-diabetes, antibacterial, anti-inflammatory, osteoporosis, antioxidation, etc. The antitumor and neuroprotective activities were newly explored in recent years. The application of herbal medicine was gradually developed in scientific methods. The medicinal value of the genus Curculigo needs to further investigate its pharmacological mechanisms. This new review offers more insights into the exploitation of the pharmacological value of the genus Curculigo.

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Norlignans and phenolics from genus Curculigo protect corticosterone-injured neuroblastoma cells SH-SY5Y by inhibiting endoplasmic reticulum stress-mitochondria pathway

Wang

Liu²,

Wei³

et al. 2022

Journal of Ethnopharmacology

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In VitroAnti-cholinesterase Activity and Mass spectrometric Analysis ofCurculigo orchioidesGaertn., Rhizome Extract

Cited by 5 publications

References 24 publications

In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Phytochemistry and Pharmacological Activity of Plants of Genus Curculigo: An Updated Review Since 2013

Norlignans and phenolics from genus Curculigo protect corticosterone-injured neuroblastoma cells SH-SY5Y by inhibiting endoplasmic reticulum stress-mitochondria pathway

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