<i>In vitro</i> and Molecular Docking Studies of DPPH with <i>Phoenix dactylifera</i> L. (Deglet-Nour) Crude Fruits extracts and Evaluation of their Antioxidant Activity

Zeroual, Samir; Daoud, Ismail; Gaouaoui, Randa; Ghalem, Saïd

doi:10.5958/0974-4150.2020.00012.7

Cited by 7 publications

(1 citation statement)

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“…Benmeddour et al, (2013) reported higher values (272 to 1175 mg GAE 100 g -1 DW) for ten Algerian cultivars studied. Comparable results were reported by Zeroual et al (2020), who observed that the best reducer was obtained by ethyl acetate extract with 118 μg EAA mg -1 extract from the Deglet Nour variety. The reducing power of a sample is determined by the electron transfer capacities of the reducers (antioxidants) in that sample.…”

Section: Reducing Powersupporting

confidence: 67%

Polyphenolic Profile and in vitro Antioxidant Activity of Three Algerian Date (Phoenix dactylifera) Varieties

Zeroual,

Gaouaoui,

Deghima

2024

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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As known, dates are packed with antioxidants and bioactive compounds and provide various health benefits, as evidenced by their varying nitrite levels. Algerian dates, on the other hand, have not been thoroughly investigated for their bioactive compounds and overall antioxidant capacity. This research aims to tap into this potential by meticulously measuring total polyphenols, flavonoids, and condensed tannins in three popular Algerian varieties (Phoenix dactylifera L.) Ksiba, Hamraya, and Deglet Nour, and the determination of their antioxidant activity using (scavenging assays of 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, reducing power, and total antioxidant capacity. The analysis showed that the three varieties of date fruits are rich in total phenolics with an amount ranging between 71±51 and 7975±389 mg of gallic acid equivalent (GAE).100 g-1 of dry weight (DW), the flavonoid amount ranged from 31±3 to 767±4 mg of quercetin equivalent (QE) 100 g-1 DW and condensed tannins between 6± 2 and 653 ±64 mg of catechin equivalent (CE) 100 g-1 dry DW. The antiradical activity was quite promising and ranged between 0.5 and 24 µg AAE mg-1 extract for DPPH and between 2 and 113 µg AAE mg-1 extract for ABTS, while the reducing power and total antioxidant capacity values ranged from 16 to 154 μg ascorbic acid (AAE) mg-1 and 39 to 68 μg AAE mg-1 extract respectively. The results of this study show that Algerian date fruit can be regarded as a potential natural source of antioxidants, with ethyl acetate serving as the best extractant solvent, resulting in higher polyphenol content and antioxidant activities.

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Section: Reducing Powersupporting

confidence: 67%

Polyphenolic Profile and in vitro Antioxidant Activity of Three Algerian Date (Phoenix dactylifera) Varieties

Zeroual,

Gaouaoui,

Deghima

2024

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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In Silico Prediction and In Vitro Cytotoxic Activity of Arbuscular Mycorrhizal Fungi Induced Zingiber officinale Var. Rubrum

Suharti

Dachriyanus

Lucida

et al. 2022

RJPT

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Objective: Ginger (Zingiber officinale) has various medicinal properties, including anti-inflammatory, antioxidant, anti-nausea/antiemetic, antibacterial, cytotoxic, and antidiabetic activities. Methods: In silico analysis was performed to predict absorption, distribution, metabolism, and excretion (ADME). Prediction of bond and bond free energy using Autodock Vina, MGL Tools and Biovia Discovery Studio. Gingerol content of Ginger-Ethanolic Extract (MGE) was determined by thin-layer chromatography densitometry and cytotoxic activity by MTT assay. Results : The Gibbs free energy value for 6-Gingerol is -7.686 (kcal/mol), and 6-Shogaol is -7.279 (kcal/mol). Prediction of hydrogen bonding at 6-Gingerol in the amino acids Arg394, Glu353, Ala350. Hydrogen bonding in shogaol on the amino acid Arg394. Statistical analysis showed that the MGE could significantly inhibit breast cancer cells T47D growth (P< 0.05) with IC50 was 16.51 ± 3.67 μg/mL. Conclusion : Based on in silico and in vitro screening Zingiber officinale var. Rubrum has the potential to be developed for anticancer treatment.

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Total Flavonoid, Total Phenolic contents and Antioxidant activity of Methanol and n-hexane extract from purple passion fruit peel

Ngibad

Herawati

Aisyah

et al. 2023

RJPT

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The utilization of passion fruit (Passiflora edulis f. edulis Sims) is still focused on the fruit, while the passion fruit peel is still not widely applied. The passion fruit peel has the potential as an antioxidant. The polarity of solvents used in the extraction process can affect the antioxidant activity of a medicinal plant. This study aims to determine in vitro antioxidant activity using DPPH and total flavonoid and phenol content of methanol and n-hexane extract from purple passion fruit peel. The powder of purple passion fruit peel was extracted using the maceration method with methanol and n-hexane solvent and a ratio of 1:12. The total flavonoids and phenols from each extract were determined using UV-Vis spectrophotometry. The in vitro antioxidant activity was performed using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) method by determining inhibitory concentration (IC50) values. The results showed that methanol and n-hexane extract yields were 4.53% and 0.76%, respectively. Furthermore, the total phenolic content of 35.95mg GA E/g extract in purple passion fruit peel was higher than the total flavonoid content of 3.25mg QE/g extract. There was an absence of total flavonoids and total phenols in n-hexane extracts. The antioxidant activity assay using the DPPH method based on IC50 values showed that methanol extract (14.63mg/L) had better antioxidant activity than n-hexane extract (37.39mg/L). In conclusion, the results suggest that methanol and n-hexane extract from Passiflora edulis f. edulis Sims can decrease free radicals and could be used as a natural antioxidant.

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In vitro and Molecular Docking Studies of DPPH with Phoenix dactylifera L. (Deglet-Nour) Crude Fruits extracts and Evaluation of their Antioxidant Activity

Cited by 7 publications

References 0 publications

Polyphenolic Profile and in vitro Antioxidant Activity of Three Algerian Date (Phoenix dactylifera) Varieties

Polyphenolic Profile and in vitro Antioxidant Activity of Three Algerian Date (Phoenix dactylifera) Varieties

In Silico Prediction and In Vitro Cytotoxic Activity of Arbuscular Mycorrhizal Fungi Induced Zingiber officinale Var. Rubrum

Total Flavonoid, Total Phenolic contents and Antioxidant activity of Methanol and n-hexane extract from purple passion fruit peel

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