In Vitro and In Vivo Antimicrobial Activity of Cymbopogon citratus (DC.) Stapf. Against Staphylococcus spp. Isolated from Newborn Babies in an Intensive Care Unit

Oliveira, João Batista da Silva; Teixeira, Manoel Araújo; Paiva, Luiz Francisley de; Oliveira, Rafaela Faria de; Mendonça, Adriana Rodrigues dos Anjos; Brito, Maria José Azevedo de

doi:10.1089/mdr.2018.0047

Cited by 11 publications

(15 citation statements)

References 21 publications

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“…Other research has described the activity of C. citratus oil toward S. aureus. Oliveira JB et al [42] showed susceptibility of S. aureus strains isolated from newborns to this oil. The minimum inhibitory and bactericidal concentrations for EO were 0.625 mg/mL in all strains tested.…”

Section: Discussionmentioning

confidence: 95%

Composition, Anti-MRSA Activity and Toxicity of Essential Oils from Cymbopogon Species

et al. 2021

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Many of the essential oils obtained from medicinal plants possess proven antimicrobial activity and are suitable for medicinal purposes and applications in the food industry. The aim of the present work was the chemical analysis of 19 essential oils (EOs) from seven different Cymbopogon species (C. nardus, C. citratus, C winterianus, C. flexuosus, C. schoenanthus, C. martinii, C. giganteus). Five different chemotypes were established by GC/MS and TLC assay. The EOs, as well as some reference compounds, i.e., citronellol, geraniol and citral (neral + geranial), were also tested for their antimicrobial and antibiofilm activity against methicillin-resistant Staphylococcus aureus (MRSA) by the microdilution method and direct bioautography. The toxicity of EOs was evaluated by Danio rerio ‘Zebrafish’ model assay. All examined EOs showed moderate to high activity against MRSA, with the highest activity noted for C. flexuosus—lemongrass essential oil, both in microdilution and direct autobiography method. Significant difference in the toxicity of the examined EOs was also detected.

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Section: Discussionmentioning

confidence: 95%

Composition, Anti-MRSA Activity and Toxicity of Essential Oils from Cymbopogon Species

et al. 2021

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“…However, different concentration ranges aiming at an optimal dose could be assayed to kill S. aureus since its lipophilic activity is increased over higher concentrations (Córdoba et al, 2019). Other studies have demonstrated that the EO of C. citratus has a good antimicrobial effect against bacteria isolated from turtle and human newborns (Silva et al, 2018;Oliveira et al, 2019). Sahal et al (2020) as a potential antifungal and antibiofilm agent that can be used to prevent contamination in silicone rubber prostheses and medical devices.…”

Section: Resultsmentioning

confidence: 99%

Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis

Costa

Prado

Carvalho-Castro

et al. 2021

Semina: Ciênc. Agrár.

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Canine otitis externa is a disease that affects the external acoustic meatus of about 20% of dogs at some point in life, without predilection for race, age, or sex. It is a multifactorial disease whose etiology involves several microorganisms, detaching Staphylococcus aureus. Antimicrobials are the basis for treating this illness; however, due to the increase in antimicrobial resistance, conventional drugs have become ineffective, requiring the search for alternative therapies. In this context, essential oils (EOs) have great therapeutic potential due to their broad antimicrobial action. This study aimed to evaluate the minimum inhibitory concentration (MIC) in order to measure the MIC50 and MIC90 of gentamicin and EOs of Eugenia caryophyllata, Thymus vulgaris, Cymbopogon winterianus, Cymbopogon citratus, and Cinnamomum cassia against 62 Staphylococcus aureus strains isolated from the external acoustic meatus of dogs. All EOs showed antibacterial action against the studied microorganisms, and their MIC50 and MIC90 were as follows: Eugenia caryophyllata, 2.42 mg mL-1 and 7.25 mg mL-1; Thymus vulgaris, 9.51 mg mL-1 and 22.94 mg mL-1; Cymbopogon winterianus, 26.78 mg mL-1 and 157.79 mg mL-1; Cinnamomum cassia and Cymbopogon citratus, lower than 16.48 and 27.81 mg mL-1, with the same MIC for all isolates. The MIC50 and MIC90 found for gentamicin were 1μg mL-1 and 8 μg mL-1. The MIC range found to antibiotic in this assay was 0.5 μg mL-1 to 128 μg mL-1, and the isolates were classified as susceptible [48 strains (77.41%) - MIC range of 0.5-4.0 μg mL-1], intermediate [eight strains (12.90%) - (MIC = 8.0 μg mL-1], or resistant [six strains (9.68%) - MIC ≥ 16 μg mL-1]. The results, according to the in vitro assays, showed that resistance to gentamicin, one of the antimicrobials most commonly used to treat canine otitis, is present in the Staphylococcus aureus population evaluated. Additionally, the tested EOs have great potential for therapeutic use, however future studies should be carried out to evaluate their in vivo efficacy.

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“…Ylang-ylang products have a wide variety of bioactivities including antimicrobial, antibiofilm, anti-inflammatory, anti-vector, insect-repellent and so on, demonstrating it to be a useful plant to agriculture and medicine [45]. Citronella was reported to have antifungal and antibiofilm activity as well as antimicrobial activity against Staphylococcus [46,47]. Extracts from Canarium were proved to be active against MRSA and P. aeruginosa [48].…”

Section: Discussionmentioning

confidence: 99%

Essential Oils with High Activity against Stationary Phase Bartonella henselae

Shi

Zhang

2019

Antibiotics

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Bartonella henselae is a fastidious Gram-negative intracellular bacterium that can cause cat scratch disease, endocarditis in humans and animals, as well as other complications, leading to acute or chronic infections. The current treatment for Bartonella infections is not very effective presumably due to bacterial persistence. To develop better therapies for persistent and chronic Bartonella infections, in this study, with the help of SYBR Green I/PI viability assay, we performed a high-throughput screening of an essential oil library against the stationary phase B. henselae. We successfully identified 32 essential oils that had high activity, including four essential oils extracted from Citrus plants, three from Origanum, three from Cinnamomum, two from Pelargonium, and two from Melaleuca, as well as frankincense, ylang-ylang, fir needle, mountain savory (winter), citronella, spearmint, elemi, vetiver, clove bud, allspice, and cedarwood essential oils. The minimal inhibitory concentration (MIC) determination of these 32 top hits indicated they were not only active against stationary phase non-growing B. henselae but also had good activity against log-phase growing B. henselae. The time-kill assay showed 13 active hits, including essential oils of oregano, cinnamon bark, mountain savory (winter), cinnamon leaf, geranium, clove bud, allspice, geranium bourbon, ylang-ylang, citronella, elemi, and vetiver, could eradicate all stationary phase B. henselae cells within seven days at the concentration of 0.032% (v/v). Two active ingredients, carvacrol and cinnamaldehyde, of oregano and cinnamon bark essential oils, respectively, were shown to be very active against the stationary phase B. henselae such that they were able to eradicate all the bacterial cells even at the concentration ≤ 0.01% (v/v). More studies are needed to identify the active components of some potent essential oils, decode their antimicrobial mechanisms, and evaluate their activity against Bartonella infections in animal models.

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In Vitro and In Vivo Antimicrobial Activity of Cymbopogon citratus (DC.) Stapf. Against Staphylococcus spp. Isolated from Newborn Babies in an Intensive Care Unit

Cited by 11 publications

References 21 publications

Composition, Anti-MRSA Activity and Toxicity of Essential Oils from Cymbopogon Species

Composition, Anti-MRSA Activity and Toxicity of Essential Oils from Cymbopogon Species

Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis

Essential Oils with High Activity against Stationary Phase Bartonella henselae

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