In vitro and in vivo evaluation of small interference RNA‐mediated gynaecophoral canal protein silencing in Schistosoma japonicum

Cheng, Guofeng; Fu, Zhiqiang; Lin, Jiaojiao; Shi, Yi; Zhou, Yanyan; Jin, Youxin; Cai, You-Min

doi:10.1002/jgm.1314

Cited by 45 publications

(43 citation statements)

References 37 publications

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“…Therefore, our results implied that the SjIAP could be functionally involved in apoptosis regulation in S. japonicum. Although direct evidences were not presented in the study to document the SjIAP partner and/or their interactions, future studies will be warranted to elucidate their roles by using RNA interference in combination with GST pull-down technique or yeast two-hybrid system (Cheng et al 2009). …”

Section: Discussionmentioning

confidence: 98%

Molecular characterizations of an inhibitor of apoptosis from Schistosoma japonicum

et al. 2010

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Apoptosis is a normal process for regulating cellular death of many organisms. Here, we molecularly characterized an inhibitor of apoptosis from Schistosoma japonicum (SjIAP). The transcription of the SjIAP predominantly occurred at the developmental stages in a final host. Functional assay indicated that the SjIAP could inhibit caspase activity either in 293T cell or in schistosome lysates. Additionally, there were differently expressed profiles of the SjIAP in S. japonicum living in different hosts. Our preliminary results suggest that the SjIAP may play important roles in parasitic living and development as well as in the host-parasite interactions, and drug target of SjIAP might be a potential for controlling schistosomiasis.

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Section: Discussionmentioning

confidence: 98%

Molecular characterizations of an inhibitor of apoptosis from Schistosoma japonicum

et al. 2010

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“…The landmark availability of the complete sequences of the S. japonicum (171) and S. mansoni (18) genomes will provide the necessary ancillary information. As well, new approaches in antigen discovery through the generation of a large schistosome transcriptome database, gene finding, and the explosion in postgenome technologies, including DNA microarray profiling, proteomics, glycomics, immunomics, and the application of RNA interference (RNAi) and novel imaging techniques (2,24,25,41,53,72,73,75,76,85,95,109,126,127,179,189,197,205,219,224,230), provide an unprecedented opportunity to identify a new generation of vaccine target molecules that may induce greater potency than the current candidate schistosome antigens (138).…”

Section: Vaccine Developmentmentioning

confidence: 99%

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

et al. 2010

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SUMMARY The potential impact of the Three Gorges Dam (TGD) on schistosomiasis transmission in China has invoked considerable global concern. The TGD will result in changes in the water level and silt deposition downstream, favoring the reproduction of Oncomelania snails. Combined with blockages of the Yangtze River's tributaries, these changes will increase the schistosomiasis transmission season within the marshlands along the middle and lower reaches of the Yangtze River. The changing schistosome transmission dynamics necessitate a comprehensive strategy to control schistosomiasis. This review discusses aspects of the epidemiology and transmission of Schistosoma japonicum in China and considers the pathology, clinical outcomes, diagnosis, treatment, immunobiology, and genetics of schistosomiasis japonica together with an overview of current progress in vaccine development, all of which will have an impact on future control efforts. The use of synchronous praziquantel (PZQ) chemotherapy for humans and domestic animals is only temporarily effective, as schistosome reinfection occurs rapidly. Drug delivery requires a substantial infrastructure to regularly cover all parts of an area of endemicity. This makes chemotherapy expensive and, as compliance is often low, a less than satisfactory control option. There is increasing disquiet about the possibility that PZQ-resistant schistosomes will develop. Consequently, as mathematical modeling predicts, vaccine strategies represent an essential component in the future control of schistosomiasis in China. With the inclusion of focal mollusciciding, improvements in sanitation, and health education into the control scenario, China's target of reducing the level of schistosome infection to less than 1% by 2015 may be achievable.

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“…Since discovery of the first miRNA (lin-4) in 1993 by Lee (Lee et al 1993) and RNA interference in 1998 by Andrew Fire (Fire et al 1998) in Caenorhabditis elegans, three different kinds of snRNAs, including small interfering RNAs (siRNAs), microRNAs (miRNAs), and piRNA (Piwi-interacting RNA), have been widely investigated in many organisms. In S. japonicum, it had been well established for a while that the usages of siRNAs were able to artificially induce specific endogenous mRNA degradation at transcript level (Cheng et al 2005b;Cheng et al 2009). Very recently, a considerable number of miRNAs have also been identified in different development stages of schistosomes and further studies indicated that the expression profiles of these miRNAs were associated with parasite development, suggesting that miRNAs could be involved in regulating schistosome development Hao et al 2010;Wang et al 2010).…”

Section: Introductionmentioning

confidence: 98%

Molecular cloning and expression profiles of Argonaute proteins in Schistosoma japonicum

et al. 2010

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Small non-coding RNAs including microRNAs and small interfering RNAs play important roles in many biological processes of many organisms. Argonaute proteins serve as a key component of the RNA-induced silencing complex for mediating miRNA/siRNA functions. In the present study, we systematically investigated Argonaute proteins in Schistosoma japonicum by using bioinformatics in combination with 5'- and 3'-Rapid Amplification of cDNA ends techniques and thus obtained three full-length cDNAs encoding Argonaute proteins, named as SjAgo1, SjAgo2, and SjAgo3, respectively. Additionally, SjAgo1/2/3 were differentially expressed in different developmental stages of schistosomes as determined by real-time RT-PCR and Western blot. Taken together, our preliminary results suggested that SjAgo1/2/3 may control gene expression during the life cycle of S. japonicum and therefore may regulate schistosome development and other biological processes.

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In vitro and in vivo evaluation of small interference RNA‐mediated gynaecophoral canal protein silencing in Schistosoma japonicum

Cited by 45 publications

References 37 publications

Molecular characterizations of an inhibitor of apoptosis from Schistosoma japonicum

Molecular characterizations of an inhibitor of apoptosis from Schistosoma japonicum

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

Molecular cloning and expression profiles of Argonaute proteins in Schistosoma japonicum

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