Molecular cloning and expression profiles of Argonaute proteins in Schistosoma japonicum

Chen, Jing; Yang, Yanping; Guo, Suxia; Peng, Jinbiao; Liu, Zongping; Li, Jiakui; Lin, Jiaojiao; Cheng, Guofeng

doi:10.1007/s00436-010-1946-3

Cited by 21 publications

(27 citation statements)

References 28 publications

(35 reference statements)

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“…The expression of SjAgo2 was up-regulated in schistosomula and female parasites, whereas SjAgo3 was highly expressed in schistosomula and male worms. Previously, Chen et al reported that the expression of SjAgo1 was significantly higher in eggs than that in adult worms and the expression of SjAgo2 and SjAgo3 was not significantly different between male and female parasites [27]. This inconsistency is likely due to different experimental conditions, especially the endogenous controls applied in the two studies.…”

Section: Resultsmentioning

confidence: 96%

“…In S. mansoni , four Argonaute proteins were identified by two groups mainly based on bioinformatic analysis, but SmAgo3 and SmAgo4 seemed to be generated from an alternatively spliced mRNA [26]. Argonaute orthologs in S. japonicum (SjAgos) have been also reported by two groups [27], [28]. Both of them tried to determine the full-length sequences of the three Argonaute proteins and described the molecular characteristics of SjAgos.…”

Section: Introductionmentioning

confidence: 99%

“…Chen et al . also reported the differential expression of SjAgos during the parasite development and suggested that SjAgos coordinated in different SRRPs may be involved in regulating schistosome development [27]. In addition, no PIWI homologue was identified in S. japonicum , though it was found in its closely related genus Schmidtea mediterranea [29], [30].…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of Argonaute Protein, Ago2 and Its Associated Small RNAs in Schistosoma japonicum

et al. 2012

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BackgroundThe complex life cycle of the genus Schistosoma drives the parasites to employ subtle developmentally dependent gene regulatory machineries. Small non-coding RNAs (sncRNAs) are essential gene regulatory factors that, through their impact on mRNA and genome stability, control stage-specific gene expression. Abundant sncRNAs have been identified in this genus. However, their functionally associated partners, Argonaute family proteins, which are the key components of the RNA-induced silencing complex (RISC), have not yet been fully explored.Methodology/Principal FindingsTwo monoclonal antibodies (mAbs) specific to Schistosoma japonicum Argonaute protein Ago2 (SjAgo2), but not SjAgo1 and SjAgo3, were generated. Soluble adult worm antigen preparation (SWAP) was subjected to immunoprecipitation with the mAbs and the captured SjAgo2 protein was subsequently confirmed by Western blot and mass spectrometry (MS) analysis. The small RNA population associated with native SjAgo2 in adult parasites was extracted from the immunoprecipitated complex and subjected to library construction. High-through-put sequencing of these libraries yielded a total of ≈50 million high-quality reads. Classification of these small RNAs showed that endogenous siRNAs (endo-siRNAs) generated from transposable elements (TEs), especially from the subclasses of LINE and LTR, were prominent. Further bioinformatics analysis revealed that siRNAs derived from ten types of well-defined retrotransposons were dramatically enriched in the SjAgo2-specific libraries compared to small RNA libraries constructed with total small RNAs from separated adult worms. These results suggest that a key function of SjAgo2 is to maintain genome stability through suppressing the activities of retrotransposons.Conclusions/SignificanceIn this study, we identified and characterized one of the three S. japonicum Argonautes, SjAgo2, and its associated small RNAs were found to be predominantly derived from particular classes of retrotransposons. Thus, a major function of SjAgo2 appears to associate with the maintenance of genome stability via suppression of retroelements. The data advance our understanding of the gene regulatory mechanisms in the blood fluke.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of Argonaute Protein, Ago2 and Its Associated Small RNAs in Schistosoma japonicum

et al. 2012

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“…Different stages of schistosome development are associated with different categories of miRNAs, suggesting that miRNAs may be involved in the regulation of schistosome development (Krautz-Peterson and Skelly, 2008; Xue et al, 2008; Chen et al, 2010; Hao et al, 2010; Luo et al, 2010; Cai et al, 2011; Simoes et al, 2011). Several lines of evidence also indirectly imply that miRNAs may regulate the pathogenesis of schistosomiasis (Cheng et al, 2013; Hoy et al, 2014).…”

Section: Potential Role Of Schistosoma Mirnas In Worm Development Andmentioning

confidence: 99%

“…First, the key components of miRNA biogenesis, including the Ago proteins (Chen et al, 2010) and Dicer (Krautz-Peterson and Skelly, 2008; Luo et al, 2010), have been shown to be differentially expressed during different stages of schistosome development. Second, some of miRNAs such as miR-36, miR-71, bantam, miR-7, and others, have been shown to specifically express in a specific stage of S. japonicum (Xue et al, 2008; Hao et al, 2010; Cai et al, 2011).…”

Section: Potential Role Of Schistosoma Mirnas In Worm Development Andmentioning

confidence: 99%

Role of microRNAs in schistosomes and schistosomiasis

Zhu

Liu

Cheng

2014

Front. Cell. Infect. Microbiol.

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Schistosomes, a class of parasitic trematode worms, cause schistosomiasis. Accumulating evidence suggests that microRNAs (miRNAs)—small, non-coding RNAs that are known to play critical regulatory roles in many organisms—may be involved in schistosome development and sexual maturation, as well as the pathogenesis of schistosomiasis. Schistosoma miRNAs, such as Bantam and miR-10, may be involved in the pathological processes of schistosomiasis, and recent studies suggest that schistosome-specific miRNAs (e.g., Bantam, miR-3479-3p) in the bloodstream of a final host could be used as biomarkers for schistosomiasis diagnosis. Furthermore, aberrant miRNAs, such as miR-223 and miR-454, can be produced by a host in response to schistosome infection, and these miRNAs may contribute to the pathogenesis of schistosomiasis-associated liver injury. Here, we summarize recent progress evaluating the relationship between schistosome miRNAs and schistosomiasis and discuss how these miRNAs can mediate the pathogenesis of schistosomiasis and be used as biomarkers for schistosomiasis diagnosis.

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ARGONAUTE SUBFAMILY GENES IN THE SMALL BROWN PLANTHOPPER, Laodelphax striatellus (HEMIPTERA: DELPHACIDAE)

Zhou

Li³

et al. 2015

Arch Insect Biochem Physiol

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Argonaute (AGO) proteins are essential catalytic components of the RNA-induced silencing complex and play central roles in RNA interference. Using a combination of bioinformatics and rapid amplification of cDNA ends (RACE) methods, putative AGO subfamily members, ls-AGO1 and ls-AGO2, were cloned and characterized from the small brown planthopper, Laodelphax striatellus. The open reading frame (ORF) of ls-AGO1 is 2,820 bp long, encoding a putative protein of 939 amino acid residues, and ls-AGO2 contains an ORF of 2,490 bp, encoding 829 amino acid residues. The expected conserved PAZ and PIWI domains, and the conserved Asp-Asp-His (DDH) catalytic triad motif in the PIWI domain were observed in both ls-AGO1 and ls-AGO2. Reverse transcription-qPCR (RT-qPCR) results showed that both ls-AGO1 and ls-AGO2 were expressed in all developmental stages of L. striatellus with highest mRNA abundance in eggs. Expression of ls-AGO1 and ls-AGO2 was significantly decreased in adult insects in response to acquisition of rice black-streaked dwarf virus by second instar nymphs. mRNA expression of ls-AGO1 was significantly downregulated in response to low and high temperatures, but expression of ls-AGO2 was only affected by low temperature. ls-AGO1 and ls-AGO2 were initially downregulated when insects were transferred from rice to maize and to the wild grass Brachypodium distachyon, but expression showed partial or complete recovery 7 days after transfer. These results document that AGO subfamily members of L. striatellus are ubiquitously expressed at different developmental stages and respond to various stresses. Thus, AGO subfamily may act in regulating the stress-response of L. striatellus by controlling related gene expression.

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Molecular cloning and expression profiles of Argonaute proteins in Schistosoma japonicum

Cited by 21 publications

References 28 publications

Identification and Characterization of Argonaute Protein, Ago2 and Its Associated Small RNAs in Schistosoma japonicum

Identification and Characterization of Argonaute Protein, Ago2 and Its Associated Small RNAs in Schistosoma japonicum

Role of microRNAs in schistosomes and schistosomiasis

ARGONAUTE SUBFAMILY GENES IN THE SMALL BROWN PLANTHOPPER, Laodelphax striatellus (HEMIPTERA: DELPHACIDAE)

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