In vitro and in vivo evaluation of electrospun cellulose acetate/gelatin/hydroxyapatite nanocomposite mats for wound dressing applications

Abstract

The present study aimed to evaluate the efficacy of cellulose acetate/gelatin/nanohydroxyapatite (CA/Gel/nHA) nanocomposite mats as the wound dressing. The dressings were prepared with electrospinning of CA/Gel solutions containing 12.5, 25 and 50 mg nHA. The dressings were evaluated regarding their water uptake capacity, morphology, tensile strength, water vapour transmission rate, wettability and cellular response with L929 cell line. The results showed that the concentration of nHA had a direct correlation with porosity, water contact angle, water uptake, water vapor transmission rate and proliferation. In vivo studies showed that all dressings had higher wound closure percent than the sterile gauze, as the control. The highest wound closure value was achieved in the CA/Gel +25 mg nHA group, which showed 93.5 ± 1.6%. The histological and the histomorphometric examinations of the wounds revealed that the CA/Gel +25 mg nHA dressing had the greatest collagen synthesis, re-epithelialization, neovascularization and also the best cosmetic appearance. Based on our finding, it could be concluded the applicability of electrospun nanofibrous CA/Gel/nHA dressings for successful wound treatment.

Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin

“…Electrospun scaffolds are constructed by various biodegradable materials including naturally‐derived polymers such as alginate, chitosan, hyaluronic acid, collagen, and synthetic‐based polymers which are poly glycolic acid, poly 3‐caprolactone, and poly lactic acid, to name but three …”

Electrospun Nanocomposites Containing Cellulose and Its Derivatives Modified with Specialized Biomolecules for an Enhanced Wound Healing

“…This polymer is under great consideration in the biomedical industry due to its biodegradability, biocompatibility, mechanical performance, non-toxicity, high affinity, good hydrolytic stability, relative low cost, and excellent chemical resistance [99]. These exceptional properties have driven the processing of CA-containing polymeric blends in the form of electrospun nanofibrous composites, in this way generating a new smart option for biotechnology and tissue engineering, drug delivery systems and wound dressing applications [25][26][27]. Electrospun CA has also been used to immobilize bioactive substances as vitamins and enzymes, biosensors, bio-separation, and affinity purification membranes, while non-porous CA have been used for stent coatings or skin protection after burns or wounds.…”

“…The introduction of chemical groups within the structure of cellulose has facilitated processing and contributed for the emergence of cellulose derivatives, like cellulose acetate (CA), which is the most common derivative that is considered by the European Committee for Standardization (CEN) as a bio-based polymer [23]. CA is a polymer that is easily soluble in common organic solvents, such as acetone, acetic acid, N,N-dimethylacetamide, and their mixtures, low-cost derivative of cellulose with excellent biocompatibility, high water adsorption capacities, good mechanical stability, non-toxicity, and can be efficiently processed into membranes, films, and fibers from either solutions or melts [21,[24][25][26][27][28]. Electrospinning allows for the production of CA-based nanomeshes with an intricate and complex architecture that can be functionalized with active biomolecules to address the specific demands of acute and chronic wounds via simple, reproducible and cost effective approaches [29][30][31].…”

Development of polypyrrole/collagen/nano-strontium substituted bioactive glass composite for boost sciatic nerve rejuvenation in vivo

“…For viability tests, cells were seeded onto nanomats covered coverslips put in 96-culture plates and permitted to develop for one day before investigation as depicted before [25]. The suitability of the dermal fibroblasts on the nanomats was resolved utilizing the colourimetric Solution Cell Proliferation Assay (MTT examine) as per maker's directions.…”