<i>In vitro</i> and <i>in vivo</i> studies with tetra‐hydro‐jasmonic acid (LR2412) reveal its potential to correct signs of skin ageing

Tran, Christian; Michelet, Jean‐François; Simonetti, Lucie; Fiat, F.; Garrigues, Alexia; Potter, Anne; Segot, E.; Watson, Rachel; Griffiths, C.E.M.; Lacharriére, Olivier de

doi:10.1111/jdv.12113

Cited by 22 publications

(24 citation statements)

References 26 publications

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“…Subjects' perception confirmed the good safety profile of Retinol/LR2412: subjects considered that the daily use of Retinol/LR2412 was safer than that of tretinoin 0.025%. These results are not unexpected as a reason for the good safety profile Retinol/LR2412 may reside in the anti‐inflammatory characteristics of LR2412 according to the LR2412 ability of inhibiting the synthesis of mediators such as IL8 and PGE2 involved in inflammation and irritation.…”

Section: Discussionmentioning

confidence: 83%

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A double‐blind randomized study comparing the association of Retinol and LR2412 with tretinoin 0.025% in photoaged skin

Bouloc

Vergnanini²,

Issa

2015

J of Cosmetic Dermatology

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Introduction Topical tretinoin is considered the gold standard to treat photoaged skin, but it is associated with side effects and only available upon prescription. Aim of the study To compare the efficacy, tolerance, and perception of a fixed proprietary combination (Retinol 0.2%/LR2412 2%) vs. tretinoin 0.025% cream in women with photoaged skin. Material/Methods In this randomized, parallel, double-blind, controlled clinical study, women applied to the entire face for 3 months in the morning a SPF 50 sunscreen and in the evening either the association of Retinol 0.2%/LR2412 2% or tretinoin 0.025%. Clinical and instrumental parameters were assessed at days 0, 28, 56, and 84. Subject perception of the efficacy, tolerance and cosmeticity of the tested products were assessed at days 28, 56, and 84. Results A total of 120 women (60 to Retinol 0.2%/LR2412 2% cream and 60 to tretinoin 0.025% cream) were included in the study. Both products improved considerably wrinkles, mottled pigmentation, pores, and global photodamage. No statistically significant differences were noted between Retinol 0.2%/LR2412 2% cream and tretinoin 0.025% cream. Adverse effects were mostly graded mild. Overall, Retinol 0.2%/LR2412 2% cream was better tolerated than tretinoin 0.025% cream. At all visits, subject perception of the association of Retinol 0.2%/LR2412 2% was either comparable to or better than tretinoin 0.025% cream. Conclusion The treatment outcome of Retinol 0.2%/LR2412 2% cream does not differ from the one of tretinoin 0.025% cream. Clinical results were not statistically different. Furthermore, Retinol 0.2%/LR2412 2% cream is better tolerated and better perceived by women used to rejuvenation procedures.

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Section: Discussionmentioning

confidence: 83%

“…LR2412 has shown in vitro and in vivo its efficacy in counteracting signs of skin aging. 13,14 Indeed, LR2412 enhances in vitro the deposit of hyaluronic acid (HA) in the basal and suprabasal layers of the epidermis. LR2412 stimulates the hyalurosome and increases HA Synthases 2 and 3 and CD44 (HA receptor) expression.…”

Section: Introductionmentioning

confidence: 99%

A double‐blind randomized study comparing the association of Retinol and LR2412 with tretinoin 0.025% in photoaged skin

Bouloc

Vergnanini²,

Issa

2015

J of Cosmetic Dermatology

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“…3D models offer an attractive alternative to current 2D in vitro and in vivo assays as they provide a more in vivo -like context than 2D assays and eliminate species difference by enabling drug testing directly in a human model system. For example, in a 3D LSE, treatment with a jasmonic acid derivative, LR2412, rescued the loss of ECM proteins observed with ageing, including collagen IV, indicating that the compound possesses ‘anti-ageing’ properties ( Tran et al, 2014 ). Plant extract 1201, which has senolytic properties, blocked the detrimental effects of the SASP from stress-induced premature senescence (SIPS) HDFs in a human LSE causing rescue of the senescent cell dependent reduction in epidermal thickness and impaired differentiation of keratinocytes ( Lammermann et al, 2018 ).…”

Section: Applications For Organotypics In Senescence Researchmentioning

confidence: 99%

Tissue engineering to better understand senescence: Organotypics come of age

Milligan

Tyler

Bishop

2020

Mechanisms of Ageing and Development

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The recent advent of ‘organs in a dish’ has revolutionised the research landscape. These 3D culture systems have paved the way for translational, post genomics research by enabling scientists to model diseases in the laboratory, grow patient-derived organoids, and unite this technology with other cutting-edge methodologies such as drug discovery. Fields such as dermatology and neuroscience have revolutionised the development of robust 3D models, which faithfully recapitulate native physiology in vivo to provide important functional and mechanistic insights. These models have underpinned a rapid growth in the number of organs and myriad of human diseases that can be modelled in 3D, which currently includes breast, cerebral cortex, heart, intestine, kidney, liver, lung, neural tube, pancreas, prostate, skin and stomach, as well as patient derived tumours. However, so far, they have not yet been employed extensively in the study of fundamental cellular programmes such as senescence. Thus, tissue engineering and 3D culture offer an exciting opportunity to further understand the bright and dark sides of senescence in a more complex and physiologically relevant environment. Below, we will discuss previous approaches to investigating senescence and ageing using organotypic models, and some potential opportunities for future research.

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“…in vitro model in a keratinocyte migration situation called "migration model" (partially mimicking the suction blister in vivo clinical model) and compared it to the existing 3D RealSkin ® regeneration standard model [6] referred here as "standard model." The standard model allows vertical regeneration (homoeostasis maintenance) to being studied while migration model introduces the additional step of re-epithelialization as observed in the phase II of wound-healing process.…”

Section: Questions Addressedmentioning

confidence: 99%

“…Adapted from the 3D RealSkin ® standard model , [6] different from the 3D organotypic reconstructed skin , [7] the living dermal equivalents …”

Section: Experimental Designmentioning

confidence: 99%

3D In vitro model of the re‐epithelialization phase in the wound‐healing process

Deshayes

Bloas

Boissout

et al. 2017

Experimental Dermatology

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Keratinocytes of the basal layer function are to maintain tissue homoeostasis and to fulfil skin repair in response to an external aggression. In wound-healing, during re-epithelialization phase, epithelial precursor cells gradually migrate from the edges of the wound. The epidermal reconstruction model called standard model allows the vertical skin regeneration process (proliferation/differentiation) to being investigated, and keratinocyte function in preserving skin homoeostasis to being assessed. Here, we developed and characterized a 3D migration model, which introduces a step of keratinocytes migration such as the one observed in the phase of re-epithelialization in woundhealing process. We validated the added value and the discriminative potential of this model by demonstrating pro-epithelializing effects of compounds. This new model allows the role of keratinocytes in different biomechanical and environmental requests to being better understood, and brings a new tool for compound screening and the study of mechanisms involved in skin regeneration. | BACKGROUNDWound-healing is a process of tissue regeneration, which involves three phases that partially overlap: (i) a vascular or inflammatory phase, (ii) a tissue repair phase and (iii) a maturation phase .[1] During phase II, keratinocytes gradually migrate from the wound edges, a period during which basal keratinocytes may differ from precursors essentially observed in the basal layer. Live imaging of stem cells recently allowed in rodent to better understand how stem cells behaviour changes in response to wounding [2] ; however, no model has succeeded in reflecting the whole wound-healing process. The current approaches consist in sequencing the process, using models that focus on one phase. In spite of its limits, this strategy, associated with clinical observations, allows the mechanisms involved to being partially described | QUESTIONS ADDRESSEDWe developed and characterized a new epidermal regeneration 3Din vitro model in a keratinocyte migration situation called "migration model" (partially mimicking the suction blister in vivo clinical model) and compared it to the existing 3D RealSkin ® regeneration standard model [6] referred here as "standard model." The standard model allows vertical regeneration (homoeostasis maintenance) to being studied while migration model introduces the additional step of re-epithelialization as observed in the phase II of wound-healing process. We studied the effect of pro-regenerating/pro-wound-healing compounds to illustrate the added value of this model. are seeded around the ring, the centre remaining empty ( Figure 1A). | EXPERIMENTAL DESIGNRing is removed 10 days after seeding the keratinocytes. Compounds to be evaluated for their capacity to impact the epidermal regeneration and epithelialization speed process are added to the culture medium. Analysis of tissue morphology, quality and thickness of the reconstructed epidermis and keratinocyte migration kinetics were performed. Experimental protocol and metho...

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In vitro and in vivo studies with tetra‐hydro‐jasmonic acid (LR2412) reveal its potential to correct signs of skin ageing

Abstract: The in vitro and in vivo data demonstrate that based on its multiple interactions within human skin, LR2412 has potential to partially correct the signs of ageing in intrinsically and photoaged skin.

Cited by 22 publications

References 26 publications

A double‐blind randomized study comparing the association of Retinol and LR2412 with tretinoin 0.025% in photoaged skin

A double‐blind randomized study comparing the association of Retinol and LR2412 with tretinoin 0.025% in photoaged skin

Tissue engineering to better understand senescence: Organotypics come of age

3D In vitro model of the re‐epithelialization phase in the wound‐healing process

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