<i>In Vitro</i>
            and
            <i>in Vivo</i>
            Evaluation of the Inflammatory Potential of Various Nanoporous Hydroxyapatite Biomaterials

Velard, Frédéric; Schlaubitz, Silke; Fricain, Jean-Christophe; Guillaume, Christine; Laurent-Maquin, Dominique; Möller-Siegert, Janina; Vidal, Loı̈c; Jallot, Édouard; Sayen, Stéphanie; Raissle, Olivier; Nédélec, Jean-Marie; Vix‐Guterl, Cathie; Anselme, Karine; Amédée, Joëlle; Laquerrière, Patrice

doi:10.2217/nnm.15.12

Cited by 14 publications

(12 citation statements)

References 43 publications

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“…Bellone et al [33] performed an in vitro study with human bone marrow cells seeded on implants coated with equine-derived bone granules and identified that the release of cytokines from the TGF-β1 family increased 1.5-fold compared to noncoated implants. In our study, the bovine derived bone in granules also promoted a slight increase in TGF-β1 release, which can be related to the hypothesis that the particle size and the shape of biomaterials are determinant for cell adherence and cytokine release [34][35][36][37]. In terms of the results obtained, an important limitation of our study is the use of primary human osteoblasts to evaluate immunologic response; hence, further studies must be conducted using monocyte or macrophage cell cultures to better compare the antigenic properties of different bone substitute presentations.…”

Section: Discussionsupporting

confidence: 69%

Cellular Response of Human Osteoblasts to Different Presentations of Deproteinized Bovine Bone

et al. 2022

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Objectives: This study evaluated the cellular response of primary osteoblasts exposed to two different presentations of a low-temperature non-sintered deproteinized bovine bone matrix (DBBM). Materials and methods: Six different baths of a commercially available DBBM block (Bonefill® Porous Block) and one of DBBM granule (Bonefill® Porous) were evaluated to identify the mineral structure and organic or cellular remnants. Samples of the same baths were processed in TRIZOL for RNA extraction and quantification. For the immunologic cell reaction assay, primary human osteoblasts (pOB) were exposed to DBMM block (pOB + B) or granules (pOB + G), or none (control) for 1, 3, or 7 days of cell cultivation. Expression of proinflammatory cytokines by pOB was evaluated by crosslinked ELISA assay. In addition, total DNA amount, as well as cell viability via LDH evaluation, was assessed. Results: Organic remnants were present in DBBM blocks; 45.55% (±7.12) of osteocytes lacunae presented cellular remnants in blocks compared to 17.31% (±1.31) in granules. In three of five batches of blocks, it was possible to isolate bovine RNA. The highest concentration of TGF-β1 was found in supernatants of pOB + G on day 7 (218.85 ± 234.62 pg/mL) (p < 0.05), whereas pOB + B presented the lowest amount of TGF-β1 secretion at the end of evaluation (30.22 ± 14.94 pg/mL, p < 0.05). For IL-6 and OPG, there was no statistical difference between groups, while pOB + G induced more IL-8 secretion than the control (3.03 ± 3.38 ng/mL, p < 0.05). Considering the kinetics of cytokine release during the study period, all groups presented a similar pattern of cytokines, estimated as an increasing concentration for IL-6, IL-8, and OPG during cultivation. Adherent cells were observed on both material surfaces on day 7, according to H&E and OPN staining. Conclusion: Neither tested material induced a pronounced inflammatory response upon osteoblast cultivation. However, further studies are needed to elucidate the potential influence of organic remnants in bone substitute materials on the regeneration process.

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Section: Discussionsupporting

confidence: 69%

Cellular Response of Human Osteoblasts to Different Presentations of Deproteinized Bovine Bone

et al. 2022

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“…Ceramics, inorganic materials formed through treatment with heat, possess crystalline structures, meaning they are often porous and brittle [269]. Commonly used ceramics include bioactive glass, which consists of a mixture of silicon dioxide, sodium oxide, calcium oxide, and phosphate oxide, [270] and hydroxyapatite, a naturally occurring material found in bone [271]. Other ceramic-based materials exhibit similar properties and these materials, along with hydroxyapatite, have been investigated extensively in combination with stem cells for tissue engineering of bone [206,[272][273][274][275][276][277][278][279][280][281][282][283][284][285][286][287][288][289].…”

Section: Ceramic-based Biomaterialsmentioning

confidence: 99%

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Willerth

Sakiyama‐Elbert

2019

STJ

112

113

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Combining stem cells with biomaterial scaffolds serves as a promising strategy for engineering tissues for both in vitro and in vivo applications. This updated review details commonly used biomaterial scaffolds for engineering tissues from stem cells. We first define the different types of stem cells and their relevant properties and commonly used scaffold formulations. Next, we discuss natural and synthetic scaffold materials typically used when engineering tissues, along with their associated advantages and drawbacks and gives examples of target applications. New approaches to engineering tissues, such as 3D bioprinting, are described as they provide exciting opportunities for future work along with current challenges that must be addressed. Thus, this review provides an overview of the available biomaterials for directing stem cell differentiation as a means of producing replacements for diseased or damaged tissues.

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“…CaP substrates were soaked in 1 mL of Dulbecco's Modified Eagle Medium (DMEM, Gibco, 10566016) at 37°C. After 15 min, 30 min, 1 h, 2 h, 4 h, 24 h and 48 h of incubation, the substrates were retrieved and the serum free DMEM was collected to assess the calcium and phosphorus ions using Induced Coupled Plasma -Optical Emission Spectroscopy (ICP-OES, iCAP 6300 duo plasma emission spectrometer) [13]. Each point of time was analysed in triplicate.…”

Section: Bioactivity Experimentsmentioning

confidence: 99%

Harnessing Wharton’s jelly stem cell differentiation into bone-like nodule on calcium phosphate substrate without osteoinductive factors

et al. 2017

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Designing materials to direct stem cell fate has a relevant impact on stem cell biology and provides insights facilitating their clinical application in regenerative medicine. Inspired by natural bone compositions, a friendly automated spray-assisted system was used to build calcium phosphate substrate (CaP). Sprayed biomimetic solutions using mild conditions led to the formation of CaP with controlled physical properties, good bioactivity and biocompatibility. Herein, we show that via optimization of physical properties, CaP substrate induce osteogenic differentiation of Wharton's jelly stem cells (WJ-SCs) without adding osteogenic supplement factors. These results suggest a great potential of sprayed CaP and WJ-SCs in bone tissue engineering and may facilitate the transition from bench to beside and the development of clinically successful engineered bone.

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In Vitro and in Vivo Evaluation of the Inflammatory Potential of Various Nanoporous Hydroxyapatite Biomaterials

Abstract: These findings highlight that the most critical physicochemical parameters for these nanoporous hydroxyapatite are, the crystallinity that controls dissolution potential, the specific surface area and the size and shape of crystallites.

Cited by 14 publications

References 43 publications

Cellular Response of Human Osteoblasts to Different Presentations of Deproteinized Bovine Bone

Cellular Response of Human Osteoblasts to Different Presentations of Deproteinized Bovine Bone

Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

Harnessing Wharton’s jelly stem cell differentiation into bone-like nodule on calcium phosphate substrate without osteoinductive factors

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